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Citations for Leica :
701 - 750 of 1310 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Brains were sliced at 7-10 µm on a CM1950 or CM3050 S cryostat (Leica) at −20°C and serial sections were placed into each Superfrost slide and stored at −20C for downstream immunostaining.
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bioRxiv - Cell Biology 2023Quote: ... Frozen tissue blocks were sectioned (5-7 μm) with a CM3050S Leica cryostat (Leica Mycrosystems). Tissues were washed three times in PBS for 5 min and incubated in PB buffer for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Frozen muscle cross sections (7-10 μm) were made using a cryostat (Leica CM 1950) and collected on Superfrost Ultra Plus slides (Thermo Fischer Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Kidneys blocks were sectioned at 5-7 µm thickness using a microtome (Leica; RM2125 RTS), placed onto microscope slides (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... Leaf transverse sections (7–10 μm) thickness cut using a rotary microtome (Leica CM3050S, Germany) and stained with toluidine blue ...
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bioRxiv - Plant Biology 2023Quote: ... Sections (7 µm) were made by using a RJ2035 microtome (Leica Microsystems, Rijswijk, The Netherland), stained 5 min in 0.1% Ruthenium Red (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... was used to section the blocks on a Leica Ultracut UCT (7) ultramicrotome (Leica Microsystems), which were collected onto copper grids ...
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bioRxiv - Neuroscience 2024Quote: ... 7-10 μm sections from the nerves were cut using a cryostat (Leica Microsystems, Germany) and collected directly onto glass slides ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were cut to 6 µm on a microtome (Leica) and mounted on SuperFrost Plus slides (Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... 6) Opal 780/anti-Ki67 (Ready-to-use, MM1, Leica). Slides were cover-slipped using ProLong™ Diamond Antifade Mountant (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... Sections ≤ 6 µm were cut using a microtome (LEICA RM2255) and stained with Hematoxylin Erythrosine Saffron staining (HES) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Neuroscience 2020Quote: ... Cryosections were prepared from tissue embedded in a 2:1 mixture of 30% sucrose: OCT (Sakura Finetek, catalog #4583) onto X-tra slides (Leica Microsystems, catalog #3800200). Tissue sections were dried ...
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bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Videorecordings were acquired at 2 frames per second (fps) using a Grasshopper camera (Point Grey) mounted on a stereomicroscope (Leica MZ6 and MZ7.5). Videos were analysed and animal speed calculated using a custom-written MATLAB software (Zentracker ...
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bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Neuroscience 2024Quote: ... fluorescence in nIII/nIV at 2 dpf, visualized using a SugarCube LED Illuminator (Ushio America, Cypress CA) on a stereomicroscope (Leica Microsystems, Wetzlar, Germany) and absence of a swim bladder at 5 dpf ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Neuroscience 2021Quote: ... adult mice (P50-60) were placed on a stereotaxic frame (Angle One; Leica, Germany). Bilateral craniotomy (1 mm in diameter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... during which 7-10 crickets were sampled each day and imaged under a stereomicroscope (Leica EZ4W) when being anesthetized with carbon dioxide ...
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bioRxiv - Cell Biology 2021Quote: ... Thin 7 μm section were obtained upon cryosection using the CryoJane tape transfer system (Leica, 39475205) and a tungsten blade ...
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bioRxiv - Immunology 2020Quote: ... The 70 nm sections obtained by thin sectioning using a Leica UC 7 microtome (Leica microsystems), were collected on formvar coated slot grids (EMS 215-412-8400 ...
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bioRxiv - Bioengineering 2019Quote: ... the slide was sputter-coated with 7 nm Pt/Pd prior to imaging (Leica ACE600 sputter). High-resolution images were obtained utilizing a LEO (Zeiss ...
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bioRxiv - Microbiology 2022Quote: ... Sections of 60 nm thickness were then cut with an Ultracut 7 ultramicrotome (Leica, Vienna, Austria), collected on copper grids with Formvar supporting membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue blocks were finally sectioned at 7 µm using a Leica microtome (Leica Microsystems, Wetzlar, Germany) and mounted on glass slides (VWR ...
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL). Slices were mounted on gelatin covered microscope slides and allowed to dry ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Microbiology 2019Quote: ... each for 30 min at room temperature and 100% for 2 × 1 h and 1 × 30 min) and dried in a Leica EM CPD030 (Leica Microsystems Belgium, Diegem, Belgium). The membranes were mounted on a stub and coated with 5 nm of carbon (Leica Microsystems Belgium ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Cancer Biology 2021Quote: ... 6-10 μm-thick sections were made on a cryostat (Leica) and deposited on gelatinized slides and immediately fixed and permeabilized in 95% EtOH ...
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bioRxiv - Neuroscience 2022Quote: ... 6-µm sections were prepared using a microtome (Leica, Wetzlar, Germany). The sections were rehydrated before immunofluorescence staining ...
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bioRxiv - Microbiology 2022Quote: ... and sliced (6 µm) using a Cryostat CM3050 S (Leica, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... antigen retrieval was performed using antigen retrieval buffer pH=6 (Leica) for 20min ...
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bioRxiv - Bioengineering 2023Quote: ... and sputter coated with 6 nm of platinum (Leica EM ACE600). The coated samples were then imaged with Hitachi S-4700 FE-SEM.
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bioRxiv - Immunology 2023Quote: ... Tissue sectioning (6 µm) was performed on a cryostat (Leica CM1860), and sections were stained with three different panels of anti-human secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... CCI injuries were produced using an electromagnetic impactor (Impact One, Leica Biosystems, Buffalo Grove, IL). The contact velocity was set at 3.5 m/s with a cortical impact depth of 1.0 mm ...
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bioRxiv - Neuroscience 2021Quote: The CCI procedure was carried out using a Leica Impact One device (Leica Microsystems Inc). Anesthetic levels were induced with 4% isoflurane gas mixed in 100% oxygen and the maintained under 2% isoflurane for the rest of the procedure ...
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bioRxiv - Neuroscience 2020Quote: ... TBI was performed with a CCI device (Impact One Stereotaxic Impactor for CCI, Leica Microsystems) equipped with a metal piston using the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... We used an electromagnetically controlled cortical impact (CCI) injury device (Impact One impactor CCI, Leica) to induce TBI in the cerebral cortex of C57BL/6 mice ...
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bioRxiv - Neuroscience 2019Quote: ... One hemisphere was cut into 60 μm coronal sections using a vibratome (Leica VT1000S, Germany) for fluorescence immunohistochemistry ...