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Citations for Leica :
151 - 200 of 944 citations for 7 Bromo 2 Methyl 1 Indanone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Biochemistry 2021Quote: ... Ultrathin (70 nm) and semi-thin (300 nm) sections were generated using a Leica EM Ultracut 7 ultramicrotome (Leica, Heerbrugg, Switzerland) and sections were post-stained using 2% uranyl acetate and lead citrate respectively ...
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bioRxiv - Immunology 2022Quote: ... they were paraffin-embedded and sectioned (7 µm thickness) on slides using a Leica RM2125 RTS microtome (Leica Biosystems, Concord, Canada). Slides were deparaffinized and washed using two rounds of toluene (T324-1 ...
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bioRxiv - Immunology 2023Quote: ... cut into 4 groups of 8 sequential 7-μm-thick sections separated by a depth of 150-μm using a Leica CM1860 UV cryostat (Leica Microsystems), and mounted as duplicates on Fisherbrand ProbeOn Plus slides glass slides (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II ...
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bioRxiv - Physiology 2019Quote: ... serial cross-sections (sections cut after on another) (7 μm) were obtained from frozen samples using a microtome (Leica Microsystems, Wetzlar, Germany). The sections were thaw-mounted on Polysine glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... For immunohistochemical staining paraffin-embedded brains were cut in 7 μm thick sagittal sections with the Leica RM2155 microtome (Leica, Wetzlar, Germany)and were rehydrated in xylene and a graded alcohol series using the Leica autostainer XL (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Pathology 2021Quote: ... and then paraffin-embedded as block tissues which were subsequently sectioned with 7-μm thickness by a microtome (RM2235, Leica Microsystems Inc.). After that ...
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bioRxiv - Neuroscience 2023Quote: ... Lung samples were embedded in paraffin wax prior to sectioning at 7 µm using a Hyrax M25 Rotary Microtome (Leica Biosystem, Germany). Sections were stained with hematoxylin and ethanol-based eosin and assessed by a veterinary pathologist who was blinded to the study designs ...
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bioRxiv - Biophysics 2023Quote: ... and/or H2O2 (10 mM) were prepared immediately before measurements and condensates formation was monitored by DIC microscopy in situ (Leica LMD 7). Each sample was filled into 1.5 mm diameter quartz capillaries ...
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bioRxiv - Neuroscience 2023Quote: ... After washing in PBS (Phosphate Buffered Saline) specimens have been embedded in 7% agarose and cut into 70-100 μm sections using a vibratome (Leica VT1000S; 81). Sections have been washed three times 10 min in 0.3% PBT (PBS with 0.3% TritonX-100) ...
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bioRxiv - Plant Biology 2023Quote: ... Disease phenotypes on the coleoptiles were assessed at 7 days post inoculation by imaging lesions on a Leica M205 FA Stereomicroscope (Leica Microsystems, UK). Each experimental replicate contained 5 biological samples for each treatment (3 mock-inoculated ...
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Neuroscience 2021Quote: ... were made from 5–7-week-old wild-type animals (C57Bl/6j, Janvier) using a vibrating blade microtome (VT1200, Leica Microsystems, Wetzlar, Germany) in NMDG based cutting solution (in mm) ...
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bioRxiv - Plant Biology 2020Quote: ... The hairy roots were embedded in 7% agarose before being transversely sectioned at a thickness of 50 μm using a vibratome (Leica VT1000S, Leica, Germany). The safranin O-green staining method was used to detect the deposition and composition of lignin(Bouvier et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
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bioRxiv - Bioengineering 2022Quote: ... Dehydrated samples were embedded in paraffin and then cut in 7 μm thick sections using a rotary microtome (HistoCore AUTOCUT, Leica Biosystems, Nussloch, Germany). Sections were deparaffinised ...
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bioRxiv - Neuroscience 2020Quote: ... and the brain was cut into 400-um thick coronal sections using a vibratome (Leica VT1000S; Leica, speed 5, vibration frequency 7-8), while in bubbling ice cold ACSF (recipe as above ...
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bioRxiv - Neuroscience 2022Quote: ... in Epitope Retrieval Solution 2 (Leica, AR9640) for 20 minutes at 100°C ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2) ...
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bioRxiv - Neuroscience 2020Quote: ... Cryosections were prepared from tissue embedded in a 2:1 mixture of 30% sucrose: OCT (Sakura Finetek, catalog #4583) onto X-tra slides (Leica Microsystems, catalog #3800200). Tissue sections were dried ...
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... Approximately 2 μl of each sample was processed by HPF using a Leica EM PACT 2 (Leica, Milton Keynes, UK). After HPF ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
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bioRxiv - Neuroscience 2023Quote: ... DCX+/CLR- and DCX-/CLR+ cells) were counted using Image J (Fiji 1.46) on 7 physical disectors obtained with confocal microscopy (Leica TCS SP5, oil immersion 63× objective) in each animal ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
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bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...