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Citations for Leica :
551 - 600 of 1370 citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Sections were cut to a thickness of 7 μm with a Leica HistoCore AUTOCUT Rotary Microtome (Leica Biosystems, Wetzlar, Germany) and Leica RM2245 ...
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bioRxiv - Bioengineering 2023Quote: ... Images were taken with either a fluorescent light microscope (Axio Observer 7, Zeiss, Oberkochen, Germany) (Figure 3B and 5A and B) or a confocal microscope (TCS SP5X, Leica Microsystems CMS GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated with a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems, 3-7 min). The neurons were considered completely photoinactivated when the membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then embedded in 7% agarose and cut into 0.3 mm sagittal sections using a vibratome (Leica VT1000s). The slices were incubated overnight at 37° C in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... One mm2 of each cortical layer was dissected out by the Leica laser capture microdissection apparatus (Leica Microsystems) and collected in a RLT lysis buffer containing 1% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: ... A single cortical contusion was made to the exposed dura mater using the Impact One impactor (Leica Biosystems) with the following injury parameters ...
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bioRxiv - Neuroscience 2023Quote: ... An electromagnetically controlled cortical impact (CCI) injury tool (Impact One stereotaxic impactor, Leica Microsystems, Buffalo Grove, IL, US) was used to perform a left sided moderate TBI at the primary motor and somatosensory cortices as we previously described 38 ...
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bioRxiv - Cancer Biology 2023Quote: The datasets were manually annotated at slide level by one of three board-certified pathologists (E.B., K.G. and G.J.) using either Aperio ImageScope (Leica Biosystems) or OMERO Plus (Glencoe Software) ...
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bioRxiv - Molecular Biology 2023Quote: Plunge freezer - one that can be used to blot grids from the back only (here: Leica EM GP)
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bioRxiv - Neuroscience 2024Quote: Closed-skull mild traumatic brain injury was administered using the Leica Impact One (Leica Biosystems, Cat. No. 39463920) controlled cortical impact (CCI ...
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bioRxiv - Molecular Biology 2019Quote: ... sectioned into 4 μm sections (Leica, Wetzlar, Germany), and stained with HE for microscopic assessment (Olympus ...
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bioRxiv - Developmental Biology 2019Quote: ... fixed in 4% PFA and cryo-sectioned (Leica) with a thickness of 12 μm ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... the paraffin-embedded block tissues were sectioned at the surface with ∼7-µm thickness by a microtome (RM2235, Leica Microsystems Inc.). The thin tissue slices were stained by H&E ...
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bioRxiv - Plant Biology 2020Quote: ... The hairy roots were embedded in 7% agarose before being transversely sectioned at a thickness of 50 μm using a vibratome (Leica VT1000S, Leica, Germany). The safranin O-green staining method was used to detect the deposition and composition of lignin(Bouvier et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Ultrathin (70 nm) and semi-thin (300 nm) sections were generated using a Leica EM Ultracut 7 ultramicrotome (Leica, Heerbrugg, Switzerland) and sections were post-stained using 2% uranyl acetate and lead citrate respectively ...
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bioRxiv - Immunology 2022Quote: ... they were paraffin-embedded and sectioned (7 µm thickness) on slides using a Leica RM2125 RTS microtome (Leica Biosystems, Concord, Canada). Slides were deparaffinized and washed using two rounds of toluene (T324-1 ...
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bioRxiv - Immunology 2023Quote: ... cut into 4 groups of 8 sequential 7-μm-thick sections separated by a depth of 150-μm using a Leica CM1860 UV cryostat (Leica Microsystems), and mounted as duplicates on Fisherbrand ProbeOn Plus slides glass slides (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Neuroscience 2021Quote: Mild TBI was induced by a standard protocol for CCI using the electromagnetic Impact One device (Leica Biosystems, IL)19 ...
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bioRxiv - Cancer Biology 2021Quote: ... the presence of one cell per well was confirmed by image acquisition using the LEICA DMI4000B microscope (Leica Microsystems) and Lumina Vision software (Mitani Corporation) ...
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bioRxiv - Neuroscience 2023Quote: ... Z-stacks were collected with a step size of 0.2 µm and deconvolved using the LAS AF One Software Blind algorithm (10 iterations) from Leica Software v3.1.0 ...
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bioRxiv - Neuroscience 2023Quote: ... medial-lateral (ML) coordinate 1.5 mm) using an electromagnetic Leica Impact One Stereotaxic impactor (Leica Biosystems, Richmond, IL, USA) with following parameters ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 4% PFA for 24 hours at 4°C and sectioned (20-60 μm) using a vibratome (Leica VT 1000s). If necessary ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S, Leica, Wetzlar, Germany) in 50 µm slices ...
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bioRxiv - Cancer Biology 2019Quote: ... sputtered coated with platinum (4 nm) (Leica EM SCD500). Tissues were imaged on a FEG-SEM Zeiss Sigma VP ...
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bioRxiv - Cell Biology 2023Quote: ... sectioned (4 um) using a cryostat CM1850 (Leica, CA), mounted onto microscope slides ...
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bioRxiv - Cell Biology 2024Quote: ... sectioned to 3-4 μm thickness (Leica RM2255, Germany), and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Physiology 2019Quote: ... serial cross-sections (sections cut after on another) (7 μm) were obtained from frozen samples using a microtome (Leica Microsystems, Wetzlar, Germany). The sections were thaw-mounted on Polysine glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... For immunohistochemical staining paraffin-embedded brains were cut in 7 μm thick sagittal sections with the Leica RM2155 microtome (Leica, Wetzlar, Germany)and were rehydrated in xylene and a graded alcohol series using the Leica autostainer XL (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Pathology 2021Quote: ... and then paraffin-embedded as block tissues which were subsequently sectioned with 7-μm thickness by a microtome (RM2235, Leica Microsystems Inc.). After that ...
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bioRxiv - Neuroscience 2023Quote: ... Lung samples were embedded in paraffin wax prior to sectioning at 7 µm using a Hyrax M25 Rotary Microtome (Leica Biosystem, Germany). Sections were stained with hematoxylin and ethanol-based eosin and assessed by a veterinary pathologist who was blinded to the study designs ...
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bioRxiv - Biophysics 2023Quote: ... and/or H2O2 (10 mM) were prepared immediately before measurements and condensates formation was monitored by DIC microscopy in situ (Leica LMD 7). Each sample was filled into 1.5 mm diameter quartz capillaries ...
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bioRxiv - Neuroscience 2023Quote: ... After washing in PBS (Phosphate Buffered Saline) specimens have been embedded in 7% agarose and cut into 70-100 μm sections using a vibratome (Leica VT1000S; 81). Sections have been washed three times 10 min in 0.3% PBT (PBS with 0.3% TritonX-100) ...
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bioRxiv - Plant Biology 2023Quote: ... Disease phenotypes on the coleoptiles were assessed at 7 days post inoculation by imaging lesions on a Leica M205 FA Stereomicroscope (Leica Microsystems, UK). Each experimental replicate contained 5 biological samples for each treatment (3 mock-inoculated ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
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bioRxiv - Neuroscience 2019Quote: ... They were then rinsed with PBS and one hemisphere for each mouse was sliced coronally at 100 μm with a vibratome (Leica) for imaging of dTomato-labeled abGC spines (Figure #) ...
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bioRxiv - Neuroscience 2020Quote: ... with one to three processes and a smooth cell membrane (Fig 2A) as visualized using an inverted microscope (DMIL; Leica) equipped with a fluorescent LED light source (X-Cite 110LED ...