Labshake search
Citations for Leica :
651 - 700 of 1523 citations for 7 AMINO 2 TERT BUTOXYCARBONYL 1 2 3 4 TETRAHYDROISOQUINOLINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+ fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software (US ...
-
bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Histological analysis of the 2 µm paraffin-embedded tissue sections was performed with the Bond Polymer Refine Detection Kit (Leica, Wetzlar, Germany) on a BondMax system (Leica) ...
-
bioRxiv - Neuroscience 2021Quote: ... the cochlea was glued on a metal block with Loctite 401 and cut to 2 halves by a vibratome (VT1200S, Leica, Wetzlar, Germany) with FREQ index at 7 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
-
bioRxiv - Bioengineering 2022Quote: Following embedding within 2% agarose and fixation overnight samples were dehydrated and embedded in paraffin using an automatic tissue processor (ASP300; Leica, London, UK). All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica) ...
-
bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
-
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
-
bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
-
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
-
bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
-
bioRxiv - Cancer Biology 2023Quote: ... the brains were removed and sectioned into 150 µm transverse slices after embedding in 2% low-melting agarose using a vibratome VT1000 (Leica, Wetzlar, Germany). The OTCs were cultured on Millicell cell cultures inserts in 6-well plates using 1 mL of FCS-free medium consisting of DMEM/F12 supplied with 1 x B27 ...
-
bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut, Leica Microsystems, Bensheim, Germany). The ultra-thin sections were post-contrasted in a LEICA EM STAIN (Leica Microsystems ...
-
bioRxiv - Neuroscience 2023Quote: ... Confocal z-stacks covering the whole depth of the neuron somata were taken with 0.38 μm step size using a 63× oil objective with 2× digital zoom magnification (Leica TCS SPE, Germany). Stacks were then processed using FIJI/ImageJ software ...
-
bioRxiv - Neuroscience 2023Quote: ... Images from the same area of the CA1 region of each hippocampus were acquired using a Leica DMLB 2 microscope (Leica, Wetzlar, Germany) connected to a Leica DFC320 FireWire digital microscope camera (Leica ...
-
bioRxiv - Neuroscience 2024Quote: ... Coronal brain sections (20 μm) were cut around the bregma 0 to -2 mm using a Leica CM1950 cryostat (Leica Biosystems, Germany). For P2X7R immunofluorescence labelling ...
-
bioRxiv - Physiology 2024Quote: ... the animals of different genotypes and stages were randomly chosen and treated with 10 mM sodium azide in M9 solution and aligned on an 2% agarose pad on slides and images were acquired using a confocal microscope (Leica TCS SPE) with a 20× ...
-
bioRxiv - Physiology 2023Quote: ... the worms were randomly chosen and treated with 10 mM sodium azide in M9 solution and aligned on an 2% agarose pad on slides and images acquired using a confocal microscope (Leica TCS SPE) with a 63× objective lens ...
-
bioRxiv - Cell Biology 2020Quote: ... was used to section the blocks on a Leica Ultracut UCT (7) ultramicrotome (Leica Microsystems), which were collected onto copper grids ...
-
bioRxiv - Plant Biology 2021Quote: ... Cross sections 7-10 μm thick were obtained using a microtome (Leica RM 2245, Leica Biosystems Nussloch GmbH ...
-
bioRxiv - Neuroscience 2020Quote: ... 7-10 μm sections from the nerves were cut using a cryostat (Leica Microsystems, Germany) and collected directly onto glass slides ...
-
bioRxiv - Cancer Biology 2020Quote: ... Colony forming efficiency was determined after 7 days using Leica DMI8 inverted microscope (Leica Microsystems) and quantified using Image J software.
-
bioRxiv - Cancer Biology 2022Quote: ... Cryosections of 7 µm were generated with a Leica cryostat (DB80 LX microtome blades, Leica) using Kawamoto tape62 (Section-lab ...
-
bioRxiv - Neuroscience 2020Quote: ... Ultrathin sections (50 nm) were cut using a UC-7 ultramicrotome (Leica Microsystems, Vienna, Austria) and contrasted with UranyLess™ (Science Services ...
-
bioRxiv - Pathology 2020Quote: ... the specimens were sectioned (7 μm) with a freezing microtome (Leica CM3050S, Leica, Wetzlar, Germany) and carefully trimmed until we found middle side of tendon ...
-
bioRxiv - Pathology 2020Quote: ... the specimens were sectioned (7 μm) with a freezing microtome (Leica CM3050S, Leica, Wetzlar, Germany) and carefully trimmed until we found middle side of tendon ...
-
bioRxiv - Biochemistry 2019Quote: ... Cryosections were performed at 7 µm sections using Cryostat LeicaCM1850 UV (Leica Biosystems, Wetzlar, Germany). For staining ...
-
bioRxiv - Immunology 2021Quote: ... Blocks were cut at 5–7 µm thickness using an RM2245 microtome (Leica, Wetzlar, Germany) and sections were mounted onto glass slides using albumin-glycerol ...
-
bioRxiv - Neuroscience 2022Quote: ... 7-µm thin transversal slices were cut on a cryostat (Reicher-Jung, Cryocut 1800; Leica) and deposited on positively charged BK-7 glass slides (Menzel-Gläser Superfrost Plus ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissue sections (7 μm) were cut using the Leica CM1850 cryosectioning device (Leica Biosystems, Germany).
-
bioRxiv - Neuroscience 2023Quote: ... Brains were sliced at 7-10 µm on a CM1950 or CM3050 S cryostat (Leica) at −20°C and serial sections were placed into each Superfrost slide and stored at −20C for downstream immunostaining.
-
bioRxiv - Biophysics 2023Quote: ... Leaf transverse sections (7–10 μm) thickness cut using a rotary microtome (Leica CM3050S, Germany) and stained with toluidine blue ...
-
bioRxiv - Cell Biology 2023Quote: ... Frozen tissue blocks were sectioned (5-7 μm) with a CM3050S Leica cryostat (Leica Mycrosystems). Tissues were washed three times in PBS for 5 min and incubated in PB buffer for 1 h at RT ...
-
bioRxiv - Cell Biology 2022Quote: ... Frozen muscle cross sections (7-10 μm) were made using a cryostat (Leica CM 1950) and collected on Superfrost Ultra Plus slides (Thermo Fischer Scientific) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Kidneys blocks were sectioned at 5-7 µm thickness using a microtome (Leica; RM2125 RTS), placed onto microscope slides (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2023Quote: ... Sections (7 µm) were made by using a RJ2035 microtome (Leica Microsystems, Rijswijk, The Netherland), stained 5 min in 0.1% Ruthenium Red (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... was used to section the blocks on a Leica Ultracut UCT (7) ultramicrotome (Leica Microsystems), which were collected onto copper grids ...
-
bioRxiv - Neuroscience 2024Quote: ... 7-10 μm sections from the nerves were cut using a cryostat (Leica Microsystems, Germany) and collected directly onto glass slides ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
-
bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
-
bioRxiv - Neuroscience 2021Quote: ... Videorecordings were acquired at 2 frames per second (fps) using a Grasshopper camera (Point Grey) mounted on a stereomicroscope (Leica MZ6 and MZ7.5). Videos were analysed and animal speed calculated using a custom-written MATLAB software (Zentracker ...
-
bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
-
bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
-
bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
-
bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
-
bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
-
bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
-
bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...