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Citations for Leica :
151 - 200 of 1936 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... blocks were cut at 7 μm using a cryostat (CM 3050S, Leica). The cryosections were placed on glass slides (SuperFrost Plus Adhesion ...
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bioRxiv - Neuroscience 2020Quote: ... 7 mM MgCl2 and 0.5 mM CaCl2) using a VT1200S vibratome (Leica). The amygdala was micro-dissected from the brain slices and sonicated in ice-cold homogenization buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... 7 mM MgCl2 and 0.5 mM CaCl2) using a VT1200S vibratome (Leica). Slices were recovered in oxygenated ACSF solution at 32°C for 2h before 1mM AHA was added to the ACSF solution for additional 2.5 h ...
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bioRxiv - Neuroscience 2023Quote: ... The frozen blocks were cryostat-sectioned into 7 μm sagittal sections (Leica). Sections were mounted on poly-L-lysine coated glass slides ...
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bioRxiv - Developmental Biology 2024Quote: ... and then serially cut into 7-µm sections on a microtome (Leica). Slides were immersed in 2N HCl for 15 min for DNA denaturation ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Plant Biology 2024Quote: ... and mounted on CryoJane CFSA 1/2 adhesive-coated glass slides (Leica) at the laser microdissection platform from Bordeaux Neurocentre Magendie (https://neurocentre-magendie.fr/) ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Developmental Biology 2021Quote: ... or 1:300 (rabbit anti-cleaved caspase 3, Cell Signaling Technology, 9665S; rabbit anti-p53, Leica Microsystems ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Cell Biology 2019Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections of 7 μm in thickness were cut by freezing microtome (Leica, Germany). The sections were used for immunofluorescence according to a previously described method (Wang ...
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bioRxiv - Neuroscience 2021Quote: ... blocks were sectioned at 90 nm thickness on an ultramicrotome (Ultracut 7, Leica), mounted on copper grids ...
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bioRxiv - Immunology 2021Quote: ... 7-10 μm thick sections were prepared with a cryostat (Leica CM 1950). For the H&E staining ...
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bioRxiv - Cell Biology 2023Quote: ... Cryosections (typically 7-10 µm thickness) were created on a Cryostat (3050S, Leica) and the sliced tissues were collected on adhesive microscope slides (16005-110 ...
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bioRxiv - Plant Biology 2023Quote: ... Resin blocks were sectioned at 7 μm with a rotary microtome (Leica Biosystems), stained on glass slides with Toluidine Blue solution for 30 seconds ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen kidney sections were cut at 7 µm thickness in a cryostat (Leica) and mounted on MAS-coated glass slides (Matsunami) ...
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bioRxiv - Pathology 2023Quote: ... Cryosections 7 μm thick were made using a Leica CM1850 cryotome (Leica, Germany), placed on Superfrost Plus glass slides (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... 7 µm thick sections were prepared using a cryotome (Leica Biosystems, Wetzlar, Germany). To detect the transplanted mFB and to analyse Cx43 expression in the scar area ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Cancer Biology 2023Quote: ... for GRPR or Bond Epitope Retrieval Solution 2 (1/100, Cat# AR9640, Leica) for Ecad ...
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bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Microbiology 2020Quote: The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections at 7 µm thickness were cut on a cryostat (Leica Biosystems, Wetzlar, Germany), mounted on positively charged slides ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... Polymerised resin blocks were sectioned using an ultramicrotome (Ultracut 7, Leica Microsystems, Wetzlar, Germany) to generate 60 nm ultra-thin sections that were collected on 200 mesh copper grids ...
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bioRxiv - Neuroscience 2022Quote: ... Embedded samples were sectioned to 7 μm sections using a microtome (Leica RM2235, UK), and mounted on HistoBond slides (StatLab ...
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bioRxiv - Cancer Biology 2020Quote: ... Biomarker conditions were as follows: Ki67 (clone MM1 DO-7 mouse monoclonal antibody, Leica) was used at a 1:200 dilution with epitope retrieval solution 2 pre-treatment for 30 min ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... Whole plate acquisition was performed using a dissecting microscope and Cell Discoverer 7 (Leica). Images were captured with ZEN ...
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bioRxiv - Developmental Biology 2020Quote: 7 μm sections were cut on a Leica CM3050 S Research Cryostat (Leica Biosystems). Tissue sections were further fixed for 5 minutes in 3.2% PFA ...
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bioRxiv - Immunology 2023Quote: ... Ultrathin sections (approximately 80-100 nm) were prepared using an EM UC 7 (Leica) and contrasted with uranyl acetate followed by lead citrate ...