Labshake search
Citations for Leica :
201 - 250 of 439 citations for 6 ethylpyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The root sections were mounted on slides in 50% glycerol. Wild type (cv. AMIGA) cluster root thin sections of 6 μm were produced using a microtome (RM2165, Leica Microsystems). They were counterstained for 5 min either with 0.05% toluidine blue or with 0.1% ruthenium red in 1X phosphate buffer saline (1X PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Neuroscience 2020Quote: ... transverse hippocampal slices (400 μm) were prepared from male C57BL/6 mice (10-12 weeks of age) using a vibratome (Leica, VT1200S). The CA3 region was cut ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was imaged at 6 hours and 12 hours after seeding using a Leica DMI 4000 B Microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2022Quote: ... then the right OB from each mouse was embedded in 6 % agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000S).
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bioRxiv - Bioengineering 2023Quote: ... The histological sections were taken at a thickness of 6 μm with a microtome (Leica RM2255 Microtome, Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... staining process involved serial repetitions of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems) or ER2 (Tris-EDTA buffer pH9 ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Immunology 2024Quote: ... tissues were subsequently embedded in OCT and stored at –80 °C before sectioning (6 μm) on a Leica CM1850 Cryostat (Leica Biosystems). Sections were incubated for 1 h ...
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bioRxiv - Biophysics 2024Quote: Figure 6(a) depicts the schematic diagram of the proposed PCT that was built on a Leica microscope body (DMi8, Leica, Germany). 25 identical LEDs (470 ± 20 nm ...
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bioRxiv - Cell Biology 2024Quote: ... Microphotographs (N>=6 fish per genotype) were acquired in a Leica DM4000 B microscope coupled to a Leica DFC425 C camera (Leica Microsystems).
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bioRxiv - Immunology 2022Quote: ... Tissue sections were incubated in 3% hydrogen peroxide (Bond Polymer Refine Detection Kit DS9800, Leica Biosystems) for 15 mins to block endogenous peroxidases ...
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bioRxiv - Developmental Biology 2021Quote: ... or 1:300 (rabbit anti-cleaved caspase 3, Cell Signaling Technology, 9665S; rabbit anti-p53, Leica Microsystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... embedded in 3% LM-agarose (Roth) and cut 250 µm-thick with a vibratome (Leica VT1000S). Slices were then transferred on Millicell inserts (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: Broad-band autofluorescence was acquired from sections cut at 3 μm using X20 objective (Leica DM550B). Sections were excited at 458 nm and fluorescence emission captured above 475 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... and glypican 3 (Cell Marque, #261M) was detected using BOND Polymer Refine Detection (Leica Biosystems, #DS9800), including a hematoxylin counterstain to visualize nuclei.
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bioRxiv - Microbiology 2019Quote: ... phase-3 position in an upright bright field-phase contrast microscope (DM-2500, Leica Microsystems, Germany). The viewer was unaware of the expected outcome of the respective stages of the samples to avoid any biasness ...
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bioRxiv - Cell Biology 2022Quote: ... and 3% water in acetone) and placed in an automated freeze substitution machine (AFS2, Leica Microsystems). A freeze substitution protocol was used as previously described16 ...
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bioRxiv - Neuroscience 2023Quote: High-pressure freezing: Reprogrammed cortical neurons were seeded and grown on 3 mm sapphire discs (Leica) until PID30 ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated with primary antibodies (Table 3) in Bond primary antibody diluent (AR9352, Leica) followed by secondary antibodies (Leica ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... followed by thin (∼3 nm) copper sputtering (Bal-tec MED 020, Leica Microsystemes SAS, Nanterre, France). Imaging was carried out using a Hitachi S800 FEG ...
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bioRxiv - Developmental Biology 2023Quote: Fixed brains were embedded in 3% noble agar gel and sectioned with the Vibratome VT1000S (Leica) at 50 μm thickness as free-floating sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% low melting agarose and cut into slices of 100 μm with a vibratome Leica VT 1000S (Leica, Bensheim, Germany). The slices were washed twice in 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The resin blocks were cut longitudinally into 6 μm slices of the femur distal metaphysis using a Leica SP1600 microtome (Leica Microsystems, Germany). Fluorescence signals of calcein and AR from the ROIs were captured using a fluorescence microscope (Q500MC ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were embedded in 6% agarose and sectioned coronally at 30-micron thickness using a Leica VT1000S vibratome (Leica Camera, Wetzlar, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Bioengineering 2022Quote: ... The distribution of cells in the microgel matrix was assessed after 6 h using a Leica SP8 DIVE multiphoton microscope (Leica Microsystems, Germany) with 16x water immersion lens ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Plant Biology 2022Quote: ... Nodule primordia and nodules were embedded in 6% low-melting agarose and sliced into 40 - 50 µm thick sections using a vibrating-blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2022Quote: ... the fresh retinal wholemount was embedded in 6% agar and cut into 250-μm slices with a vibrating blade microtome (Leica VT100 S). For nocodazole treatment ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... The resin blocks were cut longitudinally into 6-μm slices of the tibia using a Leica RM2165 rotary microtome equipped with a tungsten blade (Leica Microsystems, Germany). Fluorescence signals emitted by calcein green and alizarin red were recorded using a fluorescence microscope (Leica Microsystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
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bioRxiv - Plant Biology 2019Quote: ... Expression of the appropriate construct was analysed after 3 days by confocal laser-scanning microscopy (Leica SP8) with excitation lights of 405 nm for CFP and 488 nm for GFP.
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bioRxiv - Cell Biology 2020Quote: ... Images were prepared with the Leica Application Suite – Advanced Fluorescence (LAS-AF) Version 3 software (Leica Microsystems).
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bioRxiv - Biophysics 2019Quote: ... we imaged cilia dynamics with 3 different microscopes (two commercial spinning disk microscopes from 3i and Leica, and one 2-photon scanning microscope using the scanning artifact ...
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bioRxiv - Neuroscience 2020Quote: ... the Ca2+ signal within the SNs was captured during 3-min using Leica DM6000 microscope (Leica Microsystems) and processed with the software Fiji image J ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL) to a thickness of 30 µm ...
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bioRxiv - Cell Biology 2020Quote: ... The surface area of cardiomyocytes stained by MHC was determined with image analysis software (Leica Qwin 3) and calculated as the mean of 100 to 120 cells from randomly selected fields.
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bioRxiv - Cell Biology 2021Quote: ... were recorded and the maximal projection was deconvoluted with 3 iterations by the LAS AF 2.7.3.9 software (Leica).
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bioRxiv - Biochemistry 2021Quote: ... for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems). Datasets were collected at the NIH Multi-Institute Cryo-EM Facility (MICEF ...
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bioRxiv - Molecular Biology 2019Quote: ... A 30 second video recording was captured using a 3 MP DFC290 HD digital camera (Leica Microsystems) at a rate of 30 frames per second ...