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Citations for Leica :
251 - 300 of 1663 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was spread on the 200-μm side of a 3-mm type A gold plate (Leica Microsystems) covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems ...
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bioRxiv - Neuroscience 2019Quote: Mice were anesthetized with isoflurane inhalant gas (3%) in an induction chamber and positioned in a stereotaxic frame (myNeuroLab, Leica Microsystems Inc. ...
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bioRxiv - Physiology 2019Quote: ... IF was performed on free-floating sections obtained after the tissues were included in 3% agarose and parasagittaly sectioned (60 μm) with a vibratom (Leica). Because the fluorescence of the endogenous DsRed2 is quenched with the epitope retrieval treatments required for BrdU and PCNA staining ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in paraffin and sectioned into 3-μm thick slices using a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). The slices were floated in a 37 °C water bath and mounted either on frosted-edge slides for histological staining (Hurst Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired by confocal microscopy using a Leica SP5 system controlled by the LAS AF 3 software (Leica Microsystems). For semi-quantitative quantification of the fluorescence intensity ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035 ...
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bioRxiv - Neuroscience 2022Quote: ... embedded into 3% low melting point agarose and then sectioned into 50-60 um using a vibratome (Leica, cat# VT1000S), mounted for direct visualization of EGFP or processed for immunofluorescence as it follows ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lamps were positioned above zebrafish embryos in 6-well plates such that the power of UV irradiation was ∼5 mW/ cm2 [as measured by a hand-held power sensor (Spectroline, XDS-1000] and ∼3 mW/cm2 for cells. For all imaging experiments, a Leica M205-FA microscope equipped with a stereomicroscope was used ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was embedded in 3% agarose (in H2O) and sectioned parasagitally with 70 μm thickness using a vibratome (Leica). The slices were mounted using Vectashield H-1000 Mounting Medium (Vector ...
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bioRxiv - Plant Biology 2022Quote: ... 5-day-old wild-type Arabidopsis roots or 3-week-old true leaves were frozen in a high-pressure freezer (EM ICE, Leica), followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2 ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues from at least 3 independent mice were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH) or an LSM980 microscope and ZEN Desk 3.4 software (Carl Zeiss GmbH) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks of the substantia nigra were then embedded in 3% agarose and sectioned using a vibratome (Leica, VT1000S, Germany).
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Cell Biology 2023Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Neuroscience 2023Quote: ... which were subsequently blotted for 3 seconds and vitrified by plunging into liquid ethane using an EM-GP2 plunge-freezer (Leica). Grids were then stored in liquid nitrogen until data collection.
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... fixed brains were embedded in 3% agarose and sectioned at 50 μm thickness with a vibrating microtome (Leica VT1000S Microtome). Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were either embedded in 3% agarose for coronal sectioning at 150 µm thickness on a vibrating microtome (VT1000S; Leica) or cryoprotected samples and sectioned coronally at 20 µm on a Leica CM3050 cryostat ...
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bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
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bioRxiv - Immunology 2023Quote: ... A total volume of 3 µl was used for plunge freezing into liquid ethane using an automatic plunge freezer EM GP2 (Leica). The ethane temperature was set to −183 °C and the chamber to 24 °C with 80% humidity ...
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bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were embedded within a 3% agarose (BP160-100, Fisher Bioreagents) mixture and sectioned in a vibratome (VT 1200, Leica) into 300-µm thick sections ...
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bioRxiv - Bioengineering 2024Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Developmental Biology 2021Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto copper grids ...
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bioRxiv - Neuroscience 2020Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto copper grids ...
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bioRxiv - Developmental Biology 2020Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto copper grids ...
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bioRxiv - Cell Biology 2019Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto copper grids ...
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bioRxiv - Developmental Biology 2023Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto slot grids and post stained with 2% aqueous uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2024Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto copper grids ...
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bioRxiv - Cell Biology 2024Quote: ... on a Leica Ultracut 7 ultramicrotome (Leica Microsystems) and collected onto copper grids ...
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bioRxiv - Microbiology 2019Quote: ... The plates were kept at 4°C until quantification of the cells using an inverted microscope (Leica DM IRB). One hundred cells were counted and the number of blue stained cells among them was recorded ...
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bioRxiv - Neuroscience 2020Quote: ... Mounted sections were stored at 4°C until they were imaged using a laser scanning confocal microscope (Leica SP8).
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M phosphate buffered saline (PBS, Fisher #BP399-20) at +4°C before slicing on a freezing microtome (Leica) in a 1:12 series of 40 µm thick coronal sections ...
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bioRxiv - Developmental Biology 2020Quote: ... embedded in Moviol and stored at 4 °C until analysis using a confocal laser scanning microscope (TCS SP1, Leica)
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bioRxiv - Neuroscience 2019Quote: ... Coronal slices of the cerebellum (250 μm thick) were prepared at 4 °C using a vibratome (VS100, Leica, Germany). They were then gently transferred and stored in aCSF at room temperature (20–22 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...