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Citations for Leica :
101 - 150 of 511 citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
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bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
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bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...
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bioRxiv - Plant Biology 2020Quote: ... The division and elongation of AR founder cells were observed with a stereoscopic microscope (Leica MZ 6; Wetzlar, Germany) and ImageJ (v ...
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bioRxiv - Cell Biology 2020Quote: ... Resin was polymerised at 60°C overnight and 60 nm ultrathin sections were cut on an Ultracut 6 (Leica) ultramicrotome and imaged as described above.
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bioRxiv - Neuroscience 2019Quote: ... brains from 6-month-old 5xFAD and WT animals were coronally sectioned through the hippocampus on a vibratome (Leica; settings ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was embedded in 6% agarose and coronal sections (40 μm) were cut using a vibratome (Leica VT1200). A total of 30 sections starting from Bregma −1.22 mm (Paxinos and Franklin(2004) ...
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 6% agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000s; Wetzlar, Germany). Slices were stored in PBS and 0.05% sodium azide at 4 °C before staining.
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bioRxiv - Plant Biology 2022Quote: ... images were taken using 6-day old young seedling using Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems).
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired as z sections at 0.27 μm (using Slidebook Software 6 from Intelligent Imaging Innovations or using LCS 3D software from Leica). Images were converted into maximal intensity projections ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Developmental Biology 2019Quote: ... Serial sections of 4–6 µm thickness were cut using a Leica RM2255 rotary microtome (Leica Microsystems GmbH, Germany) with disposable blades ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were sectioned at 50µm (1 in 6 series) in the coronal plane with a vibratome (model VT1000, Leica). Sections were stored at -20°C in a cryoprotectant solution comprised of 60% glycerol and 0.01% sodium azide in 0.1M PBS.
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bioRxiv - Microbiology 2023Quote: ... Samples for milling were frozen on 2/2 200 gold mesh grids by plunge freezing in Leica EM GP2 or GP1 plunge freezer (Leica Microsystems, Germany) at 10°C and 90% relative humidity ...
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bioRxiv - Developmental Biology 2022Quote: ... using a 2-photon Leica SP8 confocal microscope (Leica).
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 CaCl2) using a vibratome (VT 1200, LEICA) and afterwards incubated in ACSF at 36 °C for 45 min ...
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bioRxiv - Plant Biology 2021Quote: ... A confocal laser scanning microscope (TCS SP5 2; Leica) and the water immersion objective lens Leica HCX IRAPO L25×/0.95W were used to visualise YFP fluorescence (excitation ...
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bioRxiv - Cell Biology 2020Quote: ... 2) Leica SP8 FALCON inverted confocal system (Leica Microsystems) equipped with a HC PL APO 63x/1.40 oil immersion lens and a temperature-controlled hood maintained at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... with Epitope Retrieval (ER) Solution 2 (AR9640, Leica Biosystems) was performed before each 1ry antibody was applied ...
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bioRxiv - Cancer Biology 2022Quote: ... with Epitope Retrieval (ER) Solution 2 (AR9640, Leica Biosystems) was performed before each primary antibody was applied ...
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bioRxiv - Systems Biology 2023Quote: ... 2) Opal 520/CD61 (Ready-to-use, 2F2, Leica), 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µm sections were obtained using microtome (Leica RM2245) picked randomly to represent different parts of the tumor.
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bioRxiv - Plant Biology 2020Quote: Plasmolysis was induced by mounting 6-day-old seedlings in 0.8 M mannitol on microscope slides and directly observed using confocal microscopy (Leica SP8). The proportion of the cell wall length in direct contact with the plasma membrane marker SYP122-mCitrine after plasmolysis was measured using Fiji after plasmolysis ...
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bioRxiv - Neuroscience 2020Quote: 200 μm-thick hippocampal sections were isolated from 16 C57BL/6 mice of P22-28 using a vibratome (Leica, VT1200S), in the presence of ice-cold oxygenated a-CSF (125mM NaCl ...
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bioRxiv - Immunology 2022Quote: Tissue samples were fixed for a minimum of 7 days in 10% neutralized buffered formalin before being removed from BSL-4 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Microbiology 2021Quote: ... The coverslips were fitted onto sample stubs using carbon adhesive tabs and sputter coated with 6 nm Iridium (Leica SCD500). Samples were imaged at 2 KV ...
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bioRxiv - Microbiology 2021Quote: Tissue samples were fixed for a minimum of 24 h in 4% PFA before being removed from BSL-3 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Immunology 2020Quote: ... Paraffin blocks were sectioned from posterior to anterior at 6-μm thickness using a rotary microtome (Leica RM2255, Wetzlar, Germany). Sections were stained using Safranin-O/Fast Green at 90-μm intervals to assess cartilage morphology in the medial tibial plateau ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Cell Biology 2023Quote: ... Transplanted embryos were mounted in 0.2% agarose in embryo medium and imaged between shield stage and 85% epiboly (6 - 9 hpf) under an inverted TCS SP8 confocal microscope (Leica) equipped with environmental chamber (Life Imaging Services ...
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bioRxiv - Cell Biology 2023Quote: ... and an Andor DU-888 X-11056 camera (1,024 x 1,024 pixels). Other live-cell images (Fig. 6) were acquired on confocal DIVE-FALCON laser scanning confocal microscope (Leica Microsystems) controlled by the Leica LAS X Life Science software using the HCX PL APO CS 63.0×/1.30 NA glycerol immersion objective and 488 nm and 633 nm lasers ...
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bioRxiv - Cell Biology 2024Quote: ... The embedded tissues were flash-frozen in liquid nitrogen for 1 minute and then sectioned into 6 μm-thick slices using a frozen microtome (Leica). The sections were stained with hematoxylin and eosin (Servicebio ...
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 CaCl2) using a vibratome (Vibracut, Leica Biosystems, Germany) followed by incubation in carbogenized aCSF for 30 min at 36°C and then kept at room temperature (~21° C ...
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bioRxiv - Plant Biology 2021Quote: ... An upright confocal laser scanning microscope (TCS SP5 2; Leica) using a 40x apochromatic water immersion lens was used to visualized YFP fluorescence (excitation ...
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bioRxiv - Microbiology 2021Quote: ... and 2% water with an AFS (Leica microsystems, Vienna, Austria). After bringing the cells to RT they were washed in acetone and gradually infiltrated with epoxy resin (Agar Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mm sections were obtained using a vibratome (Leica, VT1200S) and immobilized using glue in a polystyrene-bottom dish (TPP ...
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bioRxiv - Biophysics 2023Quote: ... 2 mm sections were obtained using a vibratome (Leica, VT1200S) and immobilized using tissue seal (histoacryl blue ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were sectioned into 6 µm thick cross sections with a Leica CM3050 S Research Cryostat (Leica Microsystems, Wetzlar, Germany), mounted on microscope slides and fixed with 1 % PFA in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).
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bioRxiv - Biochemistry 2019Quote: ... the brains of 5xFAD mice were removed and cut into 6-µm-thick slices using a CM 1950 cryostat (Leica, Germany). The brain tissues were subjected to immunostaining with anti-Iba1 antibody to visualize microglia and 3D6 to visualize Aβ by following routine immunostaining protocols ...
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bioRxiv - Bioengineering 2020Quote: ... the scaffolds were embedded in paraffin wax and 6 µm thick slices were cut using a microtome (RM2125, Leica Microsystems, UK) and mounted on glass slides (Superfrost plus ...
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bioRxiv - Plant Biology 2021Quote: ... The root sections were mounted on slides in 50% glycerol. Wild type (cv. AMIGA) cluster root thin sections of 6 μm were produced using a microtome (RM2165, Leica Microsystems). They were counterstained for 5 min either with 0.05% toluidine blue or with 0.1% ruthenium red in 1X phosphate buffer saline (1X PBS pH 7.4 ...