Labshake search
Citations for Leica :
1 - 50 of 996 citations for 6 Hydroxy 5 2 phenyldiazenyl 2 naphthalenesulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
-
bioRxiv - Neuroscience 2019Quote: ... and kynurenic acid 1 for 2-3 min and then glued on a vibratome VT1000S (Leica). The slicing chamber was filled with the same ice-cold aCSF ...
-
bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
-
Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Retrieval was accomplished using an ethylenediaminetetraacetic acid (EDTA) buffer (Bond epitope retrieval solution 2, Leica, Concord, ON, Canada), pH9 for 20 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
-
bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
-
bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
-
bioRxiv - Cell Biology 2022Quote: ... fluorescence images were captured every 2 s for 6 min using fluorescence microscopy (#AF6000-DMI6B; Leica Microsystems, Wetzlar, Germany) (objective lens ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
-
bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
-
bioRxiv - Plant Biology 2021Quote: ... the embedded samples were sectioned at 2–5 μm thickness using a rotary microtome (Leica, Wetzlar, Germany) and stained with basic fuchsin ...
-
bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
-
bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
-
bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Immunology 2020Quote: ... 2-photon laser scanning microscopy was performed with a LEICA SP5 inverted 5-channel confocal microscope (Leica Microsystems) equipped with a 25X water-immersion objective ...
-
bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
-
bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
-
bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
-
bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
-
bioRxiv - Plant Biology 2021Quote: 5 to 6-day-old seedlings were imaged using an SP8 (Leica) inverted confocal microscope ...
-
bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
-
bioRxiv - Neuroscience 2022Quote: ... in Epitope Retrieval Solution 2 (Leica, AR9640) for 20 minutes at 100°C ...
-
bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
-
bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2) ...
-
bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
-
bioRxiv - Cell Biology 2019Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
-
bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
-
bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
-
bioRxiv - Immunology 2022Quote: ... Approximately 2 μl of each sample was processed by HPF using a Leica EM PACT 2 (Leica, Milton Keynes, UK). After HPF ...
-
bioRxiv - Biochemistry 2023Quote: ... Grids were glow discharged in air for 2 min and sample (2 μL) applied in an EM GP2 plunge freezer (Leica) at 4°C and 90% humidity ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
-
bioRxiv - Cancer Biology 2020Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
-
bioRxiv - Cancer Biology 2023Quote: ... using BOND Epitope Retrieval Solution 2 (Leica Biosystems). Detection was achieved using a Bond Polymer Refine Detection® DAB chromogen kit and counterstained with hematoxylin ...
-
bioRxiv - Immunology 2023Quote: ... and BOND Epitope Retrieval Solution 2 (Leica AR9640) for MPO antibody ...
-
bioRxiv - Pathology 2023Quote: ... speed 2 (Oxford) or 0.5 mm/s (Leica), and blade angle slightly below 0° (Oxford ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
-
bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
-
bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
-
bioRxiv - Microbiology 2023Quote: ... Samples for milling were frozen on 2/2 200 gold mesh grids by plunge freezing in Leica EM GP2 or GP1 plunge freezer (Leica Microsystems, Germany) at 10°C and 90% relative humidity ...
-
bioRxiv - Developmental Biology 2022Quote: ... using a 2-photon Leica SP8 confocal microscope (Leica).
-
bioRxiv - Cancer Biology 2021Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
-
bioRxiv - Neuroscience 2021Quote: ... and 2 CaCl2) using a vibratome (VT 1200, LEICA) and afterwards incubated in ACSF at 36 °C for 45 min ...