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Citations for Leica :
101 - 150 of 150 citations for 6 Fluoropurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and an Andor DU-888 X-11056 camera (1,024 x 1,024 pixels). Other live-cell images (Fig. 6) were acquired on confocal DIVE-FALCON laser scanning confocal microscope (Leica Microsystems) controlled by the Leica LAS X Life Science software using the HCX PL APO CS 63.0×/1.30 NA glycerol immersion objective and 488 nm and 633 nm lasers ...
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bioRxiv - Cell Biology 2024Quote: ... The embedded tissues were flash-frozen in liquid nitrogen for 1 minute and then sectioned into 6 μm-thick slices using a frozen microtome (Leica). The sections were stained with hematoxylin and eosin (Servicebio ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were sectioned into 6 µm thick cross sections with a Leica CM3050 S Research Cryostat (Leica Microsystems, Wetzlar, Germany), mounted on microscope slides and fixed with 1 % PFA in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).
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bioRxiv - Biochemistry 2019Quote: ... the brains of 5xFAD mice were removed and cut into 6-µm-thick slices using a CM 1950 cryostat (Leica, Germany). The brain tissues were subjected to immunostaining with anti-Iba1 antibody to visualize microglia and 3D6 to visualize Aβ by following routine immunostaining protocols ...
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bioRxiv - Bioengineering 2020Quote: ... the scaffolds were embedded in paraffin wax and 6 µm thick slices were cut using a microtome (RM2125, Leica Microsystems, UK) and mounted on glass slides (Superfrost plus ...
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bioRxiv - Plant Biology 2021Quote: ... The root sections were mounted on slides in 50% glycerol. Wild type (cv. AMIGA) cluster root thin sections of 6 μm were produced using a microtome (RM2165, Leica Microsystems). They were counterstained for 5 min either with 0.05% toluidine blue or with 0.1% ruthenium red in 1X phosphate buffer saline (1X PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Neuroscience 2020Quote: ... transverse hippocampal slices (400 μm) were prepared from male C57BL/6 mice (10-12 weeks of age) using a vibratome (Leica, VT1200S). The CA3 region was cut ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was imaged at 6 hours and 12 hours after seeding using a Leica DMI 4000 B Microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2022Quote: ... then the right OB from each mouse was embedded in 6 % agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000S).
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bioRxiv - Bioengineering 2023Quote: ... The histological sections were taken at a thickness of 6 μm with a microtome (Leica RM2255 Microtome, Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... staining process involved serial repetitions of the following for each biomarker: epitope retrieval/stripping with ER1 (citrate buffer pH 6, AR996, Leica Biosystems) or ER2 (Tris-EDTA buffer pH9 ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Immunology 2024Quote: ... tissues were subsequently embedded in OCT and stored at –80 °C before sectioning (6 μm) on a Leica CM1850 Cryostat (Leica Biosystems). Sections were incubated for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Microphotographs (N>=6 fish per genotype) were acquired in a Leica DM4000 B microscope coupled to a Leica DFC425 C camera (Leica Microsystems).
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bioRxiv - Biophysics 2024Quote: Figure 6(a) depicts the schematic diagram of the proposed PCT that was built on a Leica microscope body (DMi8, Leica, Germany). 25 identical LEDs (470 ± 20 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% low melting agarose and cut into slices of 100 μm with a vibratome Leica VT 1000S (Leica, Bensheim, Germany). The slices were washed twice in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The resin blocks were cut longitudinally into 6 μm slices of the femur distal metaphysis using a Leica SP1600 microtome (Leica Microsystems, Germany). Fluorescence signals of calcein and AR from the ROIs were captured using a fluorescence microscope (Q500MC ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were embedded in 6% agarose and sectioned coronally at 30-micron thickness using a Leica VT1000S vibratome (Leica Camera, Wetzlar, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Bioengineering 2022Quote: ... The distribution of cells in the microgel matrix was assessed after 6 h using a Leica SP8 DIVE multiphoton microscope (Leica Microsystems, Germany) with 16x water immersion lens ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Plant Biology 2022Quote: ... Nodule primordia and nodules were embedded in 6% low-melting agarose and sliced into 40 - 50 µm thick sections using a vibrating-blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2022Quote: ... the fresh retinal wholemount was embedded in 6% agar and cut into 250-μm slices with a vibrating blade microtome (Leica VT100 S). For nocodazole treatment ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... The resin blocks were cut longitudinally into 6-μm slices of the tibia using a Leica RM2165 rotary microtome equipped with a tungsten blade (Leica Microsystems, Germany). Fluorescence signals emitted by calcein green and alizarin red were recorded using a fluorescence microscope (Leica Microsystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
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bioRxiv - Pathology 2022Quote: ... Pictures of 4-6 different fields per sample were taken under a Leica DM5000 B microscope equipped with a digital CCD (Leica Camera, Wetzlar, Germany).
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bioRxiv - Cell Biology 2021Quote: ... resin blocks were mounted onto SEM pins with silver epoxy and sputter coated with 6 nm of iridium (Leica ACE 600, Vienna, Austria) at a 45° angle with rotation on a planetary stage to ensure the entire block was coated.
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bioRxiv - Neuroscience 2023Quote: ... Brain regions containing the possible contact sites were re-embedded into resin blocks and 70 nm thick sections were cut with an ultra-microtome (Leica EM UC 6). Electron microscopic images were taken either with a Hitachi H-7100 transmission electron microscope or a FEI series electron microscope to reveal synaptic contacts between BFPVNs and target cell types in the MS ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cell Biology 2021Quote: ... was deposited on an EM copper grid (400-mesh, Plano G2400C, coated with 6 nm thick carbon produced with a Leica EM ACE600 sputter coater), incubated for 30-60 sec and manually blotted with filter paper ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on an FE-SEM (Zeiss Merlin ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Immunology 2023Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...