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Citations for Leica :
401 - 450 of 769 citations for 6 FLUORO 2 METHYLQUINOLINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Briefly, mice were anesthetized with isoflurane (3% induction, 1.5% maintenance and secured on a stereotaxic frame (Leica Microsystems, Wetzlar, Germany). A 3 mm craniotomy (−1 AP mm bregma ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT ...
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bioRxiv - Cell Biology 2021Quote: ... 7F and S5D are average projections of time-lapse movies acquired a maximum speed during 3 minutes using a spinning-disk microscope composed of an inverted microscope (DMI4000B; Leica) equipped with an HCX Plan Apochromat 100 × /1.46 NA oil objective (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S ...
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bioRxiv - Cell Biology 2021Quote: ... Grids were blotted between 2.5 and 3 sec and plunged frozen in liquid ethane using a Leica vitrification plunger (EM GP2, Leica Microsystems). For RiboRad6mix ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were fixed with 3% formaldehyde and permeabilized with 0.1% Triton X-100 and imaged in Leica Fluorescence microscope (Leica Microsystems). Images were analyzed by ImageJ software.
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... After fixation brains were embedded in 3% agarose (Genaxxon Bioscience) and coronal slices of ~50 μm thickness were obtained using a vibratome (VT 1000S, Leica). Slices were stored in PBS at 4°C until they were stained and mounted on microscope slides.
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bioRxiv - Neuroscience 2021Quote: ... the pituitaries were embedded in 3% agarose (H2O) and para-sagittally sectioned with 60 μm thickness using a vibratome (Leica). From a single pituitary ...
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bioRxiv - Cell Biology 2021Quote: ... Dried samples were then coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The sample grids were imaged using a JEOL JEM2800 scanning/transmission electron microscope at 200 kV.
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bioRxiv - Biochemistry 2020Quote: ... Protein mixture (5 μL) were deposited onto the grids and blotted for 3 seconds using an Automatic Plunge Freezer (Leica).
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Immunology 2021Quote: ... The fixed specimens were embedded directly in paraffin blocks and cut into sections with 3–5 μm thickness using a rotary microtome (Leica RM 2125RTS ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was spread on the 200-μm side of a 3-mm type A gold plate (Leica Microsystems) covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems ...
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bioRxiv - Neuroscience 2019Quote: Mice were anesthetized with isoflurane inhalant gas (3%) in an induction chamber and positioned in a stereotaxic frame (myNeuroLab, Leica Microsystems Inc. ...
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bioRxiv - Physiology 2019Quote: ... IF was performed on free-floating sections obtained after the tissues were included in 3% agarose and parasagittaly sectioned (60 μm) with a vibratom (Leica). Because the fluorescence of the endogenous DsRed2 is quenched with the epitope retrieval treatments required for BrdU and PCNA staining ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in paraffin and sectioned into 3-μm thick slices using a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). The slices were floated in a 37 °C water bath and mounted either on frosted-edge slides for histological staining (Hurst Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired by confocal microscopy using a Leica SP5 system controlled by the LAS AF 3 software (Leica Microsystems). For semi-quantitative quantification of the fluorescence intensity ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... embedded into 3% low melting point agarose and then sectioned into 50-60 um using a vibratome (Leica, cat# VT1000S), mounted for direct visualization of EGFP or processed for immunofluorescence as it follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lamps were positioned above zebrafish embryos in 6-well plates such that the power of UV irradiation was ∼5 mW/ cm2 [as measured by a hand-held power sensor (Spectroline, XDS-1000] and ∼3 mW/cm2 for cells. For all imaging experiments, a Leica M205-FA microscope equipped with a stereomicroscope was used ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was embedded in 3% agarose (in H2O) and sectioned parasagitally with 70 μm thickness using a vibratome (Leica). The slices were mounted using Vectashield H-1000 Mounting Medium (Vector ...
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bioRxiv - Plant Biology 2022Quote: ... 5-day-old wild-type Arabidopsis roots or 3-week-old true leaves were frozen in a high-pressure freezer (EM ICE, Leica), followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2 ...
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bioRxiv - Plant Biology 2022Quote: ... The samples were then coated with 3 nm platinum and 4 nm carbon using the ACE600 coating device (Leica Microsystems).
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: ... Tissues from at least 3 independent mice were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH) or an LSM980 microscope and ZEN Desk 3.4 software (Carl Zeiss GmbH) ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks of the substantia nigra were then embedded in 3% agarose and sectioned using a vibratome (Leica, VT1000S, Germany).
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Cell Biology 2023Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Neuroscience 2023Quote: ... which were subsequently blotted for 3 seconds and vitrified by plunging into liquid ethane using an EM-GP2 plunge-freezer (Leica). Grids were then stored in liquid nitrogen until data collection.
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... fixed brains were embedded in 3% agarose and sectioned at 50 μm thickness with a vibrating microtome (Leica VT1000S Microtome). Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were either embedded in 3% agarose for coronal sectioning at 150 µm thickness on a vibrating microtome (VT1000S; Leica) or cryoprotected samples and sectioned coronally at 20 µm on a Leica CM3050 cryostat ...
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bioRxiv - Immunology 2023Quote: ... A total volume of 3 µl was used for plunge freezing into liquid ethane using an automatic plunge freezer EM GP2 (Leica). The ethane temperature was set to −183 °C and the chamber to 24 °C with 80% humidity ...
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bioRxiv - Developmental Biology 2024Quote: ... Live VL3/VL4 NMJs in abdominal hemi-segment 3 were imaged in ambient temperature using a Stellaris 5 laser-scanning confocal microscope (Leica) with a water-immersion objective (HC FLUOTAR L VISIR 25x/0.95 ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated with a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems, 3-7 min). The neurons were considered completely photoinactivated when the membrane potential reached 0 mV ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were embedded within a 3% agarose (BP160-100, Fisher Bioreagents) mixture and sectioned in a vibratome (VT 1200, Leica) into 300-µm thick sections ...
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bioRxiv - Bioengineering 2024Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Immunology 2022Quote: ... Mouse tissues (at least 2 mm2/organ) were imaged by confocal microscopy (SP8, Leica) using 40x oil objective ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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bioRxiv - Systems Biology 2021Quote: ... Mice were anesthetized with 2% isoflurane in oxygen using a precision vaporizer (Leica Biosystems). In each group ...
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bioRxiv - Plant Biology 2021Quote: ... and then transferred at −90 °C into an AFS 2 freeze-substitution device (Leica). Grafts were incubated in a cryosubstitution mix containing only uranyl acetate 0.1 % in pure acetone for 30 h (freeze substitution and embedding protocol adapted from Nicolas et al. ...
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bioRxiv - Genetics 2021Quote: ... Stained 2-day-old animals were analysed by a vertical fluorescent microscope (DM2500, Leica). We selected the area between two pharyngeal bulbs to evaluate the number of β-amyloid deposits ...
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bioRxiv - Developmental Biology 2022Quote: Widefield images were acquired every 2 min with an M205 fluorescent dissection microscope (Leica) equipped with a PLANAPO 2.0x objective ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% FBS) using a Leica EM PACT2 high-pressure freezing unit (Leica Microsystems) with rapid transfer system at high pressure (2100 bar) ...
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bioRxiv - Developmental Biology 2022Quote: ... They were fixed by high pressure freezing with the EMPACT-2 system (Leica Microsystems). Cryosubstitution (FS ...
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bioRxiv - Biophysics 2021Quote: ... These dauer samples were then transferred to carriers of 3 mm diameter and 0.1 mm depth and rapidly frozen in a high-pressure freezing machine (Leica, EM ICE). For automated freeze substitution ...