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Citations for Leica :
601 - 650 of 1098 citations for 6 Diazo 5 6 dihydro 5 oxo 1 naphthalenesulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000; Leica Microsystems, Heidelberg, Germany) at high magnification (x200 or x400 depending on the immunolabeled density of cells) ...
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bioRxiv - Zoology 2022Quote: ... and sectioned serially into 5 μm transverse or coronal tissue sections using the Leica X Multicut semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). Additional samples from juvenile T ...
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bioRxiv - Plant Biology 2022Quote: Sections of 5 or 10 μm were prepared on a Leica JUNG RM2055 microtome using a microtome knife (Leica Disposable blades TC-65). The sections were imaged without staining or after staining with Safranin O (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Pathology 2023Quote: ... The tissue samples were decalcified and embedded in paraffin before the preparation of coronal sections of 5-μm thickness around the ligation site using a microtome (RM2245, Leica Microsystems, Wetzlar, Germany). Tissue sections were then subjected to TRAP staining using an acid phosphatase leukocyte kit (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Cell Biology 2023Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence (TIRF) microscope (Leica AM TIRF inverted microscope; Leica Microsystems, Wetzlar, Germany). Image sequences of individual particles (500 frames ...
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bioRxiv - Physiology 2024Quote: ... H-1000) and visualized using a Leica Stellaris 5 Confocal Microscope equipped with a 40x oil-immersion objective (Leica Microsystems Inc, Illinois, USA). Fluorescence intensity profiles across sarcomeres were plotted offline using the FIJI software.
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bioRxiv - Evolutionary Biology 2023Quote: Rehydrated brains were embedded in agarose gel (5% UltraPure agarose power in deionised water) and sectioned using a vibrating blade microtome with an 80 µm interval (Leica VT1200, Wetzlar, Germany). Sections were then washed six times in PBS-TX (0.5% Triton X-100 detergent in phosphate-buffered saline) ...
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bioRxiv - Developmental Biology 2021Quote: ... Heart tissues fixed in 10% formalin and paraffin-embedded were sliced into serial 5-µm sections using a Leica RM2125 RTS microtome (Leica Camera AG, Wetzlar, Germany). Sections on slides were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2022Quote: ... The plates containing the pseudostratified epithelia were incubated at 37 °C with 5% CO2 in the 3i Marianis/Yokogawa Spinning-Disk Confocal microscope (Leica Microsystems, Sugar land, USA) as reported in our previous publications (53 ...
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bioRxiv - Cell Biology 2022Quote: ... Paraffin-embedded WAT was sliced into 5 μm sections using a Thermo Scientific™ HM 355S Automatic Microtome and mounted on Surgipath® X-tra® Microscope Slides (Leica Biosystems). To represent the whole tissue ...
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bioRxiv - Microbiology 2022Quote: ... The target probe was validated on reproductive tracts from mice experimentally inoculated with Chlamydia muridarum strain Nigg.5 Slides were stained on an automated stainer (Leica Bond RX, Leica Biosystems) with RNAscope 2.5 LS Assay Reagent Kit-Red (322150 ...
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bioRxiv - Neuroscience 2020Quote: ... equilibrated with 95 % O2-5% CO2) and parasagittal spinal cord slices (280 μm) were prepared with a vibratome (VT1200S, Leica Microsystems AG, Wetzlar, Germany) in the same solution ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in ice-cold 95% O2/5% CO2-equilibrated sACSF and sectioned at 250 µm in the sagittal plane with a vibratome (VT1200S; Leica Microsystems Inc., IL, USA). Slices were then transferred to a holding chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC staining for CAV1 was performed on formalin-fixed paraffin-embedded HER2-expressing gastric tumor sections (5 μm thickness) on a Leica Bond RX automated stainer (Leica Biosystems, Buffalo Grove, IL). Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0 ...
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bioRxiv - Plant Biology 2022Quote: To generate the training set we used two different stereo microscopes equipped with an integrated camera for image capture (ZEISS Stemi 305 with a 1.2 Megapixel integrated colour camera; Leica MZ16 coupled with a Leica CLS100x white light source and a Leica DFC420 5 Megapixel colour camera). For the downstream experiments using the adapted CNNs we only used the ZEISS Stemi 305 since it is easier to operate and to transport to the field ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor, Leica Biosystems, Richmond, IL, USA) with a 3mm round tip at a speed of 3m/s ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... The images were captured by the Leica STELLARIS 5 confocal microscope with 63X oil immersion objective ((Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X.
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bioRxiv - Evolutionary Biology 2023Quote: ... The number of fluorescent and non-fluorescent worms was counted in triplicates in droplets of 5-20 µl depending on worm density using a fluorescent dissecting scope (Leica Microsystems GmbH, Wetzlar, Germany). Competitive fitness was determined as relative proportion of non-fluorescent mutant worms compared to the fluorescent reference strain CL2122.
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed and incubated in periodic acid (Leica 38016SS4A) followed by the Schiff Reagent (Fisher SS32-500).
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20×, NA 0.30, Leica; and 10×, NA 0.25, Nikon DiaPhot 300). Images were collected by CCD cameras (Sentech or Hamamatsu C8484-05G) ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: ... The z-stack images of the whole tissue were acquired with a 5 mm step size in a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Images were segmented and volumetrically reconstructed with Imaris software (Bitplane ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... or LNPK KO hSO assembled with unlabeled hCO) were imaged under environmentally controlled conditions (37°C, 5% CO2) on a confocal microscope (Leica Stellaris, 20x objective, 2x zoom). The samples were placed in the controlled environment for 1 hour before imaging ...
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bioRxiv - Microbiology 2023Quote: FRAP experiments were performed using Stellaris 5 confocal microscope equipped with 63× oil immersion objective (Leica HC PL APO 63×/1.40 Oil CS2, NA 1.4). A 488 nm argon laser at 100% power was used to bleach a disk-shaped area of 1 μm in AMP-polyA condensates ...
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bioRxiv - Microbiology 2023Quote: ... or Leica Stellaris 5 confocal microscope (confocal fluorescence imaging) equipped with 63× oil immersion objective (Leica HC PL APO 63×/1.40 Oil CS2, NA 1.4).
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bioRxiv - Cell Biology 2023Quote: ... Acquisition of microscopy images was performed with the Stellaris 5 confocal laser scanning microscope using the 40X oil immersion objective lens (Leica HC PLA APO, 1.3 NA).
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bioRxiv - Cell Biology 2023Quote: ... Acquisition of microscopy images was performed with the Stellaris 5 confocal laser scanning microscope using the 40X oil immersion objective lens (Leica HC PLA APO, 1.3 NA).
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bioRxiv - Biophysics 2020Quote: ... they were transferred to a lab-built heated stage perfused with 5% CO2 and mounted on a confocal microscope (Leica TCS SP8 with a 10x objective). With this setup ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... The immunofluorescence images were captured on the Olympus BX60 wide-field microscope with Olympus DP70 camera or Leica STELLARIS 5 confocal microscope with a 63X oil immersion objective (Leica; HC PL APO 63x/1.40 OIL CS2) using Leica Application Suite X software (Leica ...
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bioRxiv - Biophysics 2020Quote: ... and the plates were transferred to a lab-built heated stage perfused with 5% CO2 and mounted on a confocal microscope (Leica TCS SP8 with a 10x 0.4 NA objective). With this setup ...
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bioRxiv - Biophysics 2024Quote: ... The imaging was performed with a Leica Stellaris 5 confocal microscope equipped with a 63× oil immersion objective (Leica HC PL APO 63×/1.40 Oil CS2, NA 1.4). To perform fluorescence recovery after photobleaching experiments ...
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bioRxiv - Neuroscience 2023Quote: ... titrated to pH 7.4 with concentrated hydrochloric acid) using a VT1200s vibrating microtome (Leica). The transcardial perfusion procedure with 30 ml of chilled NMDG-HEPES aCSF was performed on P60 animals prior brain dissection and slicing ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor ...
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bioRxiv - Microbiology 2022Quote: ... femur and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a tissue processor (Leica ASP6025 ...
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bioRxiv - Bioengineering 2021Quote: ... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit ...
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Retrieval was accomplished using an ethylenediaminetetraacetic acid (EDTA) buffer (Bond epitope retrieval solution 2, Leica, Concord, ON, Canada), pH9 for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... frozen sapphire sandwiches were incubated acetone containing 0.1% tannic acid at –90 °C for 20 – 24 h inside an AFS machine (Leica) under constant agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then incubated with DAPI for nucleic acid staining and imaged using a SP8 fluorescence microscope (Leica).
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bioRxiv - Microbiology 2022Quote: ... The apple tissue containing acervuli were stained with cotton blue in lactic acid and examined with a Leica DM2000 LED microscope (Leica Microsystems Switzerland ...
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bioRxiv - Plant Biology 2019Quote: ... 20% glycerol and 20% ethylic acid) to wash off green color and then observed and imaged under a DVM6a 3D microscope (Leica, Germany).
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bioRxiv - Bioengineering 2021Quote: ... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit, Leica Microsystems) for 60 min ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Genetics 2019Quote: ... Periodic acid Schiff (PAS) staining was performed by the MSKCC Molecular Cytology Core Facility using the Autostainer XL (Leica Microsystems, Wetzlar, Germany) automated stainer for PAS with hematoxylin counterstain and coverslips were mounted with Permount (Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...