Labshake search
Citations for Leica :
251 - 300 of 1121 citations for 6 BROMOPYRIDO 2 3 D PYRIMIDINE 2 4 1H 3H DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S ...
-
bioRxiv - Microbiology 2020Quote: ... After immunolabelling the grids were embedded with a mixture of 50% methylcellulose 2% and 50% sucrose 2.3M and then vitrified by plunge freezing with EMGP plunge freezer (Leica) at 30 °C and 90% humidity.
-
bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S ...
-
bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and photographed with an Aperio AT 2 Leica Scanner and an Aperio Scan Scope Console program (Leica, Wetzlar, Germany) available at the Northern Finland Biobank Borealis ...
-
bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
-
bioRxiv - Pathology 2022Quote: ... Super-resolution images were recorded on the confocal laser scanning microscopy LSM 980 equipped with Airyscan 2 (Leica, Germany).
-
bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
-
bioRxiv - Developmental Biology 2020Quote: Embryos or neural tube explants are fixed in 3.7% formaldehyde for 2 hours and sliced using a vibratome (Leica). Proteins were detected on 50 μm vibratome sections ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
-
CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Collected aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system with Cy5 channel (Leica). CXCR4 co-staining was done after fixation using whole mount staining protocol described above.
-
bioRxiv - Cell Biology 2019Quote: ... Cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI) and imaged with a Leica TCS SP5 Confocal Laser Scanning Microscope (Leica) using a 40X water immersion objective.
-
bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
-
bioRxiv - Cell Biology 2020Quote: ... then transferred to brass specimen carriers and high pressure frozen with an EMPACT 2 (Leica Biosystems, Buffalo Grove, IL).
-
bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut ...
-
bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Slides were imaged on an inverted Leica TCS SP5 confocal microscope using a 63x/1.40 oil objective with 2-3x digital zoom at calibrated magnifications and recorded with LAS AF software (Leica). Quantitation of dextran and LAMP-1 colocalization with bacteria was analyzed with CellProfiler 3.1.9 (27 ...
-
bioRxiv - Biochemistry 2022Quote: ... Plunging was performed at 90-100% humidity via automatic backside blotting (via Leica Microsystems Cryo GP 2 plunge freezer) or dual-side blotting (Mark IV FEI/Thermo Fisher Scientific Vitrobot) ...
-
bioRxiv - Developmental Biology 2023Quote: ... outside of the necrotic region) (Figure 8-figure supplement 1E-F) from 2 sections with the Application Suite Core v2.4 software (Leica).
-
bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... eMyHC (Leica NCL-MHC-d;1:200), laminin (Sigma-Aldrich L9393 ...
-
bioRxiv - Microbiology 2023Quote: ... under a stereoscope (EZ4 D, Leica, Germany). Using sterilised dissection needles ...
-
bioRxiv - Cell Biology 2024Quote: ... eMyHC (Leica NCL-MHC-d; 1:200) for staining of muscle cryosections ...
-
bioRxiv - Microbiology 2019Quote: ... 6 and 24hpi by fluorescent stereomicroscopy (Leica M205FA). 15 z slices were taken at 130x magnification ...
-
bioRxiv - Biophysics 2021Quote: ... 6-10 μm cryosections were obtained (Leica CM1520). For immunofluorescence labelling ...
-
bioRxiv - Biophysics 2020Quote: ... S cerevisiae (Sc) cells strain #2 after ~5h of starvation were high pressure frozen (HPM010, AbraFluid) and freeze substituted (EM-AFS2, Leica) with 0.1% uranyl acetate in acetone for 55h at −90°C ...
-
bioRxiv - Biochemistry 2022Quote: ... Neutrophils were then counted at the site of transection at 2 hours post injury (hpi) using a fluorescence dissecting stereomicroscope (Leica).
-
bioRxiv - Plant Biology 2019Quote: ... Abnormal colony morphology was screened by scanning plate cultures on TAP medium solidified with 2% Bacto agar under a dissecting microscope (S8APO, Leica) in a mutant pool generated by insertional mutagenesis ...
-
bioRxiv - Developmental Biology 2019Quote: ... 2 simultaneous cameras (TuCam Flash4.0, Hamamatsu) and a water immersion objective (Leica 20X, N.A. 0.75 or Leica 40X, N.A. 1.1). 1ms exposure was used for red blood cells imaging and 20ms exposure for valve structure and reporter activity experiments ...
-
bioRxiv - Cancer Biology 2019Quote: ... Sections were stained using a Leica BOND-MAX-TM autostainer with BondTM epitope retrieval 1 or 2 and the Bond Refine Detection Kit (Leica). Primary antibodies are listed in Supplementary Table S1.
-
bioRxiv - Physiology 2019Quote: ... Slides were deparaffinized and rehydrated on board before pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 15 minutes ...
-
bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
-
bioRxiv - Developmental Biology 2021Quote: ... Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
-
bioRxiv - Plant Biology 2021Quote: ... The cross section of 2-4μm was cut with a glass knife using the ultramicrotome Leica EM UC7 (Leica Microsystems). The semi-thin sections were stained for 30mins in 0.1% aniline blue in 0.1M K3PO4 at RT ...
-
bioRxiv - Plant Biology 2021Quote: ... The stained sections were observed within 1hr to detect callose deposition using confocal laser scanning microscopy (TCS SP5 2; Leica).
-
bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
-
bioRxiv - Immunology 2022Quote: Staining for SARS-CoV-2 antigen was achieved on the Bond RX automated system with the Polymer Define Detection System (Leica) used per manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
-
bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: ... Thin sections from the area of 2-photon imaging were cut with a diamond knife on an EM UC6 ultramicrotome (Leica), collected on pioloform-coated copper Synaptek slot grids (Electron Microscopy Sciences) ...
-
bioRxiv - Microbiology 2022Quote: ... Approximately 2 µL of the spore suspension per sample was then processed with Leica EM ICE HPF apparatus (Leica Microsystems), and then freeze substituted and embedded in Lowicryl HM20 (Electron Microscopy Sciences ...
-
bioRxiv - Plant Biology 2022Quote: ... We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm) with glass knives on an ultramicrotome Leica Ultracut UCT (Leica Microsystems) stained with a solution containing 1% (w/v ...
-
bioRxiv - Cell Biology 2020Quote: ... Images were captured using Metamorph software at every 15 minutes in 37C (PeCon Tempcontrol 37-2) using confocal spinning disk system (Leica DMI 6000 B equipped with YOKOGAWA CSU-W1 Confocal Scanner Unit ...
-
bioRxiv - Neuroscience 2019Quote: ... Then a metal head plate was mounted (Narishige CP-2, Japan) to allow stereotaxic fixation under the two-photon microscope (Leica SP8 MP ...
-
bioRxiv - Neuroscience 2019Quote: ... incubated in dichloromethane and then transferred to a clearing solution (benzyl alcohol: benzyl benzoate (1:2)) and imaged with a confocal laser scanning microscope (SP8, Leica).
-
bioRxiv - Neuroscience 2021Quote: ... embedded in 2% LTE agarose/ Tris-buffered saline (TBS) and cut coronally in 50 µm sections on a vibratome (VT 1000S, Leica). Sections underwent antigen retrieval in a solution of 0.1 M sodium citrate (pH 6) ...
-
bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
-
bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...