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Citations for Leica :
101 - 150 of 249 citations for 6 Amino m ethylphenol hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 300 µm thick coronal slices from the prefrontal and somatosensory cortices were obtained with a vibrating slicer (Microm VT1200 S, Leica) and incubated for at least 20 min at 38 °C in holding artificial cerebro-spicnal fluid (ACSF ...
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bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
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bioRxiv - Neuroscience 2023Quote: ... acute V1 slices (400-μm thick) from P28–32 (bath-application) and P27–42 (uncaging) mice were cut with a vibratome (VT 1200, Leica) in ice-cold slicing solution containing (in mM ...
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bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Images were taken with a digital camera (Canon EOS Rebel SL3, Canon USA) attached to a stereomicroscope (Leica M-Series). The total length of each copepod (from anterior of eye spot to fork in caudal furca ...
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bioRxiv - Neuroscience 2023Quote: ... fitted with a CMOS camera with 3.45 μm/pixel resolution (Basler ace acA2440-75 m) and a 0.63x objective lens (Leica Planapo 0.63x). Alternating dual light illumination was generated using an OptoLED system (Cairn Research ...
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bioRxiv - Neuroscience 2024Quote: ... were imaged at 2 µm depth intervals with a 0.75 NA 20X objective on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). To quantify the percentage of co-labeling between cells expressing tdTomato ...
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bioRxiv - Cell Biology 2020Quote: ... Serial thin sections (100 nm) were cut using a Leica UC 6 ultramicrotome (Leica, Wetzlar, Germany) from the surface of the block until approximately 4-5 microns in to ensure complete capture of the cell volumes ...
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bioRxiv - Developmental Biology 2020Quote: ... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
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bioRxiv - Developmental Biology 2021Quote: ... The dynamics of embryo spreading were examined for 6 hours under a Leica M165C (Leica, German) microscope coupled to a Leica DFC420 (5.0 MP ...
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bioRxiv - Biochemistry 2023Quote: ... Samples embedded in Epon EmBed812 resin were ultrasectioned (80nm) with an ultramicrotome (Leica EM UC 6) and placed on copper mesh 300 previously coated with uranyl acetate and lead citrate ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut into 300μm thick (for whole-cell experiments) or 500μm thick (for field recordings) coronal sections using a vibratome (VT1000S, Leica, Nussloch, Germany). Slices were transferred to an incubation chamber filled with warmed ACSF containing (in mM) ...
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bioRxiv - Cell Biology 2021Quote: ... Yeast slurry was transferred to the 200 µm recess of an aluminum platelet (Engineering Office M. Wohlwend) and placed in an HPM100 (Leica Microsystems) for high pressure freezing ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 80 µl of the M solution and 5 µl were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany). The data was analyzed using the Leica Application Suite X imaging and analysis software (Leica ...
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bioRxiv - Systems Biology 2022Quote: High-resolution confocal images with 0.333 µm z-step were acquired using a Leica DMi8 microscope with a 93X glycerol objective and LAS X software (Leica Microsystems). The region of interest was fixed on the hippocampal subregion CA1.
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bioRxiv - Bioengineering 2019Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm and 100 ms impact duration (Impact ONE; Leica Microsystems). The skin was sutured and the animals were placed in a 37°C incubator until consciousness was regained ...
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bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
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bioRxiv - Neuroscience 2021Quote: ... and 60 µm thick coronal slices containing the CA1 region of hippocampus were cut on an oscillating blade vibratome (Leica VT1000, Leica, Germany) and transferred to PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 200 μm coronal brain slices containing nuclei of the SOC including the MNTB and VNTB were cut with a vibratome (Leica VT1200S) in cold aCSF+KA ...
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bioRxiv - Neuroscience 2023Quote: Twenty-μm-thick coronal sections containing the MS and the CPU were prepared with a Leica SM 2000R freezing microtome (Leica Microsystems) and stored at -20 °C in 24-well tissue culture plates containing antifreeze solution [30% ethylene glycol ...
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bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating brain sections were then prepared by sectioning these fixed brains into 40-μm-thick sections in the coronal plane with a Leica VT-1000 Vibrating microtome or Sliding Microtome SM-2000R (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
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bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
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bioRxiv - Neuroscience 2024Quote: Mice were sacrificed by cervical dislocation and sagittal slices (250-400 μm) were prepared with an angle of 10-15° to maintain rostrocaudal striatonigral projections 82 using a vibrating microtome (Leica VT1200). Slice preparation was performed in ice-cold sucrose-based artificial cerebrospinal fluid (ASCF ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted by centrifugation at ∼800 RCF for five minutes and high-pressure frozen in the 200-µm recess of an aluminum platelet (Engineering Office M. Wohlwend 241) using an HPM100 (Leica Microsystems). The samples were then freeze substituted in 0.1% uranyl acetate ...
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bioRxiv - Biophysics 2024Quote: ... Dissected egg chambers were high-pressure frozen in gold-coated copper Type A carriers (Engineering office M. Wohlwend) using the HPM010 (Wohlwend) or the EM Ice high-pressure freezing systems (Leica Microsystems). The egg chambers were added into the 100 µm or 200 µm cavity of the planchette and 20% Ficoll (70 kDa ...
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bioRxiv - Cell Biology 2020Quote: ... Semi-thick (300-400 nm) serial sections were cut with a UC-6 ultramicrotome (Leica Microsystems, Vienna) using a diamond knife (Diatome ...
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bioRxiv - Microbiology 2019Quote: ... Ultrathin sections (approximately 50-60 nm) were cut by a Leica EM UC 6 (Leica, Wien, Austria). Sections were stained with uranyl acetate followed by lead citrate and imaged in a Tecnai 12 Spirit Bio TWIN transmission electron microscope (FEI Company ...
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bioRxiv - Cancer Biology 2021Quote: ... Embedded tissues were sectioned into 6-µm sections and loaded into BOND RXm (Leica Biosystems, Wetzlar, Germany) for a series of staining including F4/80 ...
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bioRxiv - Neuroscience 2019Quote: ... unfixed C57BL/6 mouse brains (N = 3) were embedded in tissue freezing medium (Leica Biosystems, Richmond, UK), frozen on a dry ice ethanol bath ...
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bioRxiv - Neuroscience 2021Quote: Coronal sections of the rat brains were cut at 6 μm using a microtome (Leica RM2245, Germany). Dewaxed and rehydrated hippocampal sections were blocked in TBST (TBS with Tween20 ...
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bioRxiv - Cell Biology 2019Quote: ... embedded in 6% agarose and sectioned into 100 µm thin slices using a vibratome (VT1000 S, Leica). Tissue sections were further immunostained as described above.
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bioRxiv - Cell Biology 2020Quote: ... the wafers were coated with 6 nm of carbon via a high vacuum film deposition instrument (Leica) to avoid charging ...
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bioRxiv - Bioengineering 2022Quote: ... embedded in paraffin wax and sliced at a 6 μm thickness using a microtome (RM-2125RT, Leica). Slices were mounted on slides and stained with Picrosirius Red (PSR) ...
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bioRxiv - Neuroscience 2022Quote: ... embedded in 6% agarose cubes and cut into 60 µm coronal sections using a vibratome (Leica VT1200). Sections of Vglut2-ires2-FlpO/Chx10-Cre animals were immunhistochemically stained (primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumor tissue was sectioned at 6 μm in a Leica CM 1850 Cryostat (Leica Microsystems GmbH). The sections were mounted on uncharged glass slides without the use of embedding media and placed immediately in 70% ethanol for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... Thin sections (70-90nm) were cut on a Leica Ultracut UC-6 ultramicrotome (Leica Microsystems, Wetzlar, Germany). Sections were collected on 3mm copper grids and subsequently stained for 30min with 2% aqueous uranyl acetate and for 5min with Reynold’s lead citrate ...
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bioRxiv - Physiology 2023Quote: ... Slides were sectioned at 6 µM on a Leica CM3050 S cryostat (Leica Biosystems; Deer Park, IL) and stored at −80°C until use ...
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bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections (roughly 70 nm thick) were prepared using a Leica EM UC 6 ultramicrotome (Leica, Wetzlar, Germany) and observed using a JEM-1400 Flash transmission electron microscope (JEOL ...
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bioRxiv - Immunology 2024Quote: ... RV tissue was embedded in paraffin and 6 μm sections were cut using a microtome (Leica, Germany) and slides dried in a heated chamber ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal 20μm-think brain sections that contained the barrel cortex were cut and collected on-slide (wS1) using a cryostat (Leica, CM3050 S). The slides were then stored at −80°C until further processing ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.