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Citations for Leica :
601 - 650 of 853 citations for 5 Nitrobenzothiazol 2 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: we measured the teleochrysalid abdomen surface area (Troscianko, 2014) from high-resolution photos (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD).
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Cancer Biology 2019Quote: ... a minimum of 5 images were taken of cells per experiment using a 20x water objective on an inverted confocal microscope (Leica TCS SP8). Using phalloidin staining and Image J software ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were allowed to rest in cold oxygenated (95% O2/5% CO2) aCSF and then sliced coronally using a vibratome (Leica VT1000 S). Brain slices (400-μm thick sections ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Genetics 2022Quote: ... H&E staining was performed on 5 µm sections from paraffin-embedded retinas according to standard protocols for light microscopy and photomicrography (Leica, Wetzlar, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then imaged at 37°C in a chamber supplemented with humidified 5% CO2 through white light laser confocal microscope TCS SP8 (Leica, Wetzlar, Germany). Movie S1 was processed by Bitplane-Imaris 9.5 (Andor Technology ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Bioengineering 2021Quote: ... The samples washed in PBS (3x 5 min) and embedded in Mowiol prior to confocal laser scanning microscope analysis (Leica TCS SP8X). Collagen fiber organization was determined as described in the supplementary information (supplementary Fig.S2-4 and supplementary Eq.S1.).
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bioRxiv - Immunology 2021Quote: ... Lung tissue was embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and 5 μm sections were cut on CM1950 Cyrostat (Leica, Weltzlar, Germany). Sections were blocked with 1% Rat serum and 1% FcBlock in PBS for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Microbiology 2022Quote: ... cells were imaged using a Stedycon optical setup (Abberior Instruments GmbH) integrated with Leica SP 5 microscope (Leica Microsystems GmbH, Manheim, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Fixed brains were washed three times for 5 min with PB and 150 µm-thick coronal sections were cut with a vibratome (Leica Microsystems, VT1000S). Tissue sections were labeled with DiIC18 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal and para-sagittal slices (300 μm) were prepared in ice-cold oxygenated (95% O2 / 5% CO2) ACSF with a vibratome (LeicaVT1000S, Leica Biosystems, Germany), then warmed to 36°C for 45 mins ...
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bioRxiv - Neuroscience 2022Quote: ... Delineation of CA1.SR and MDG subregions were performed using light microscope under 5 × objective lens, the newCAST software (Visiopharm, Hørsholm, Denmark) modified for stereology with a digital camera (Leica DFC 295, Germany) and a motorized stage (Ludl Mac 5000 ...
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bioRxiv - Plant Biology 2022Quote: ... The abaxial side of the leaf sample was imaged using a 63× objective (Leica, HC PL APO CS2 5 63×/1.20 Water). GFP was excited with a 488 nm WLL laser and collected at 503-530 nm ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were collected onto cover glasses and coated with gold with sputter current 30 mA for 5 seconds (Leica EM ACE200). Cover glasses were sealed on aluminum stabs using carbon tape ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm each of carbon and iridium (Leica ACE 600). Prepared samples were imaged on a Zeiss Merlin FE-SEM using an InLens detector at 1.2 – 1.5 kV and 400 – 500 pA depending on the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems, Nussloch, Germany). Brain sections were labelled with Anti-FLAG M2 F1804 ...
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bioRxiv - Plant Biology 2023Quote: ... Stems were rehydrated first in ethanol 50% and later in water and then embedded in agarose 5% before being transversally cut using a vibratome (Leica vt 1000s) to obtain 120 micron thickness sections stored in water ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the nuclei were stained with DAPI (1:2000) and the images were captured with 63X oil immersion objective by the confocal microscope (Leica STELLARIS 5). The measurements related to cell shape analysis of the nuclei in apical ...
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bioRxiv - Developmental Biology 2023Quote: The images were initially processed in Fiji with a customed macro to batch open the lif files (Image taken on Leica Stellaris 5) and split the images into individual channels ...
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bioRxiv - Biophysics 2020Quote: ... S cerevisiae (Sc) cells strain #2 after ~5h of starvation were high pressure frozen (HPM010, AbraFluid) and freeze substituted (EM-AFS2, Leica) with 0.1% uranyl acetate in acetone for 55h at −90°C ...
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bioRxiv - Biochemistry 2022Quote: ... Neutrophils were then counted at the site of transection at 2 hours post injury (hpi) using a fluorescence dissecting stereomicroscope (Leica).
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bioRxiv - Plant Biology 2019Quote: ... Abnormal colony morphology was screened by scanning plate cultures on TAP medium solidified with 2% Bacto agar under a dissecting microscope (S8APO, Leica) in a mutant pool generated by insertional mutagenesis ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 simultaneous cameras (TuCam Flash4.0, Hamamatsu) and a water immersion objective (Leica 20X, N.A. 0.75 or Leica 40X, N.A. 1.1). 1ms exposure was used for red blood cells imaging and 20ms exposure for valve structure and reporter activity experiments ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were stained using a Leica BOND-MAX-TM autostainer with BondTM epitope retrieval 1 or 2 and the Bond Refine Detection Kit (Leica). Primary antibodies are listed in Supplementary Table S1.
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bioRxiv - Physiology 2019Quote: ... Slides were deparaffinized and rehydrated on board before pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 15 minutes ...
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Plant Biology 2021Quote: ... The cross section of 2-4μm was cut with a glass knife using the ultramicrotome Leica EM UC7 (Leica Microsystems). The semi-thin sections were stained for 30mins in 0.1% aniline blue in 0.1M K3PO4 at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The stained sections were observed within 1hr to detect callose deposition using confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Neuroscience 2020Quote: ... Spinal cords were removed from the spinal column by hydraulic extrusion and embedded in low-melting point agarose (2-3%) before sectioning using a vibratome (Leica) into 300 µm thick coronal slices in ice-cold oxygenated ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Immunology 2022Quote: Staining for SARS-CoV-2 antigen was achieved on the Bond RX automated system with the Polymer Define Detection System (Leica) used per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
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bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Thin sections from the area of 2-photon imaging were cut with a diamond knife on an EM UC6 ultramicrotome (Leica), collected on pioloform-coated copper Synaptek slot grids (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2 µL of the spore suspension per sample was then processed with Leica EM ICE HPF apparatus (Leica Microsystems), and then freeze substituted and embedded in Lowicryl HM20 (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm) with glass knives on an ultramicrotome Leica Ultracut UCT (Leica Microsystems) stained with a solution containing 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured using Metamorph software at every 15 minutes in 37C (PeCon Tempcontrol 37-2) using confocal spinning disk system (Leica DMI 6000 B equipped with YOKOGAWA CSU-W1 Confocal Scanner Unit ...
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bioRxiv - Neuroscience 2019Quote: ... Then a metal head plate was mounted (Narishige CP-2, Japan) to allow stereotaxic fixation under the two-photon microscope (Leica SP8 MP ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in dichloromethane and then transferred to a clearing solution (benzyl alcohol: benzyl benzoate (1:2)) and imaged with a confocal laser scanning microscope (SP8, Leica).