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Citations for Leica :
1 - 50 of 850 citations for 4 Nitro n propan 2 yl aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Plant Biology 2022Quote: ... leaves were harvested at 3 dpi for aniline blue/calcofluor staining and analysis by epi-fluorescence microscopy (Leica DM6B ...
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... were prepared from Sprague Dawley rats (n=3; 3-4 weeks old; Janvier Laboratories) and cut using a vibratome (Leica VT1200S) in an ice-cold oxygenated ...
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bioRxiv - Genetics 2024Quote: ... a 5x N Plan objective (Leica) was used in the gap junction knockdown experiments ...
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bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
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bioRxiv - Biophysics 2020Quote: ... 2.5x (Leica 2.5x N PLAN, NA 0.07); microscopes ...
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bioRxiv - Biophysics 2020Quote: ... 2.5x (Leica 2.5x N PLAN, NA 0.07) and 10x (Nikon 10x PLAN FLUOR PH2 DLL ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Plant Biology 2023Quote: ... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
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bioRxiv - Biochemistry 2021Quote: ... A 5X objective (NA = 0.12, Leica N Plan), a 10X objective (NA = 0.25 ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Artemia individuals (n = 47) were observed and photographed (Leica EZ4HD stereomicroscope with integrated HD camera ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Cancer Biology 2024Quote: ... immunostaining of formalin-fixed paraffin-embedded sections (2-4 μm) was performed with the Bond RX automated stainer (Leica Biosystems, Germany). Antigen retrieval was 20 mins at 100°C using the Bond Epitope Retrieval solution 2 (EDTA based buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2024Quote: ... and a 20x air objective (N Plan L 566035, NA = 0.40; Leica). Imaging was performed through the implanted GRIN lens (500-µm diameter ...
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bioRxiv - Bioengineering 2024Quote: ... then sectioned into 80 n slices using an ultramicrotome (Leica Microsystems UC7) and imaged on a FEI Tecnai T12 TEM ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
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bioRxiv - Physiology 2024Quote: ... directing it to the infinity-corrected objective (Leica 2.5 N Plan, NA=0.7). The stirring mirror and the telescope were adjusted to achieve homogeneous illumination at the objective’s working distance ...
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bioRxiv - Microbiology 2024Quote: ... It was outfitted with an oil immersion objective (Leica N PLAN 50x/0,90 OIL), a fiber-coupled mercury lamp ...
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bioRxiv - Biophysics 2021Quote: N&B imaging was performed on a TCS SP8 confocal microscope (Leica Biosystems, Wetzlar, Germany) equipped with a HyD hybrid detector in photon counting mode ...
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bioRxiv - Immunology 2024Quote: ... kidneys were fixed overnight at 4°C in paraformaldehyde 4% and embedded in paraffin (Leica, Wetzlar, Germany). Sections of 5 µm were dewaxed ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal ultrathin sections (70 nm thick) were prepared using an ultramicrotome (Ultracut N ultramicrotome, Leica Microsystems) and stained with uranyl acetate for 5 min and Reynold’s lead citrate solution for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections were prepared using a Porter Blum ultramicrotome (Reichert-Nissei ULTRACUT-N; Leica, Wetzlar, Germany) and mounted on a copper grid (200 mesh ...
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bioRxiv - Neuroscience 2021Quote: ... with immersion oil conforming to ISO 8036 with a diffraction index of n=1.5180 (Leica Microsystems, Germany). For gSTED imaging ...
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bioRxiv - Neuroscience 2021Quote: ... with immersion oil conforming to ISO 8036 with a diffraction index of n=1.5180 (Leica Microsystems, Germany). Emitted light was detected with two HyD detectors in photon counting mode (Leica Microsystems ...
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bioRxiv - Plant Biology 2023Quote: ... Leica DMI6000B equipped with a Leica N Plan L 20×/0.4 CORR PH1 objective (Leica, Wetzlar, Germany) and a Hamamatsu Orca camera (model C4742-80-12AG ...
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bioRxiv - Neuroscience 2022Quote: Images (n=5 per brain area per mouse) were acquired using a SP8 confocal microscope (LEICA, Germany) with a 63x oil immersion objective (PL APO CS2 ...
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bioRxiv - Developmental Biology 2023Quote: ... used for CBs and a x10 objective (N PLAN 10x/0.25 achromatic corrected objective, Leica Microsystems CMS) used for OZ and AM ...