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Citations for Leica :
1 - 50 of 1818 citations for 4 Fluoro 5 1 pyrazolyl 2 nitrobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Cell Biology 2020Quote: ... acid alcohol (Leica) for 5 s ...
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bioRxiv - Cell Biology 2024Quote: ... 4 to 5-week-old colonies were imaged using the stereo microscope (M205 FA, Leica), and the area was determined by applying thresholding to convert reflected light 8-bit images to 1-bit images and analyzed using Fiji software (ImageJ 1.54f ...
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bioRxiv - Plant Biology 2023Quote: Nodules were embedded in 4-5% agarose and sliced in 100um sections using a vibratome (Leica). Samples were immediately imaged on slides using a 20x air objective using the excitation and emission settings described above ...
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Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organizationbioRxiv - Genetics 2020Quote: ... Figure 4 and Figure S3): 5-10 adult worms were placed on an uncharged slide (Surgipath Leica) in 5µl M9 solution ...
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bioRxiv - Biophysics 2024Quote: ... After blotting for 4-5 s in a Leica EM GP2 plunge freezer (Leica Microsystems, Wetzlar, Germany) with the chamber set to 5°C and 95% humidity ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Physiology 2024Quote: ... apparatus The tissues were embedded in paraffin (Roth) sliced in 4-5 μm sections using a microtome (Leica). The sections underwent deparaffination and rehydration procedures ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were paraffin-embedded and cut into 4–5 μm sections using a microtome (RM2125, Leica; Germany). These sections were then stained with hematoxylin and eosin (H&E ...
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bioRxiv - Plant Biology 2024Quote: ... The equatorial sections of BY-2 cells estradiol induced 5-d-old BY-2 cells expressing XVE:CbPINa:GFP or XVE:CbPINc:GFP were acquired by Leica SP8 confocal microscope (HC PL APO CS2 63×/1.2 W objective ...
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bioRxiv - Plant Biology 2023Quote: ... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
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bioRxiv - Plant Biology 2022Quote: ... Stained seed coats were fixed with 4-5% agarose gel and dissected by microtome (Leica, VT1000 S, Wetzlar, Germany) and were visualized by Nikon 80i-epi-microscope ...
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bioRxiv - Plant Biology 2021Quote: ... the embedded samples were sectioned at 2–5 μm thickness using a rotary microtome (Leica, Wetzlar, Germany) and stained with basic fuchsin ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Physiology 2021Quote: ... tissue was fixed in 4% buffered paraformaldehyde and samples subsequently paraffin-embedded and 5 μm sections prepared on a microtome (Leica). Adipose sections were viewed at 20 x magnification ...
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bioRxiv - Plant Biology 2020Quote: Seventy µm-thick transversal sections of the midrib of leaflets 4 or 5 from L1 leaf were cut using a vibratome (Leica) and stained with periodic acid (1% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
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bioRxiv - Immunology 2020Quote: ... 2-photon laser scanning microscopy was performed with a LEICA SP5 inverted 5-channel confocal microscope (Leica Microsystems) equipped with a 25X water-immersion objective ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were fixed with 4% PFA for 1 h prior to vibratome (Leica) sectioning at 100 μm ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Novolink DAB Substrate Buffer (Leica Biosystems, 1:20, 5 min). Finally ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... Olfactory bulbs were dissected and post-fixed in 1% PFA for 2-7 days and embedded in 5% agarose and sectioned into 50µm slices using a vibratome (VT1000S, Leica). Free-floating slices were washed with PBS and incubated in 5% normal goat serum in PBS/Triton/azide (0.25% Triton ...
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bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...