Labshake search
Citations for Leica :
1 - 50 of 819 citations for 3 1H Pyrrol 1 yl phenyl methanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
-
bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
-
bioRxiv - Microbiology 2020Quote: ... for 1h at RT before being imaged on an SP8 Lightning laser scanning microscope (Leica) with a HC PL APO CS2 40x/1.30 lens.
-
bioRxiv - Immunology 2024Quote: ... ýIII-Tubulin (1:250) and human HLA-DR (1:250, LN-3, Leica/Novocastra) followed by staining with a cocktail of DAPI and fluorochrome-conjugated secondary antibodies and mounting on histological slides.
-
bioRxiv - Neuroscience 2021Quote: ... snap frozen in a dry-ice chilled methanol bath and sectioned in a cryostat (Leica) at 18-μm thickness (unless otherwise specified) ...
-
bioRxiv - Immunology 2024Quote: ... Samples were then snap frozen on a dry ice-methanol ice bath in OCT (Leica), with Swiss rolls being cryosectioned (Leica ...
-
bioRxiv - Immunology 2021Quote: ... through LEICA BOND RX 1h room- temperature (RT) and developed with Bond Polymer Refine Detection (Leica, DS9800). For hematoxylin and eosin (H&E ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were transferred into cold methanol (−90°C) in a freeze-substitution chamber (Leica EM AFS). Methanol was replaced three times before the specimens were immersed overnight in anhydrous methanol at −90°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were dehydrated in methanol at -80°C and embedded by freeze-substitution (Leica EM AFS2) in Lowicryl HM 20 (Electron Microscopy Science ...
-
bioRxiv - Immunology 2022Quote: ... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
-
bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
-
bioRxiv - Cancer Biology 2022Quote: ... tissues were washed and incubated for 1h RT with the primary antibody diluted in IHC Antibody Diluent (Novocastra, Leica RE7133). Subsequently ...
-
bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
-
bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
-
bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue blocks were warmed to −20°C for at least 1h prior to sectioning and sectioned at 18 µm using a cryostat (Leica CM3050S) at −20°C ...
-
bioRxiv - Bioengineering 2022Quote: ... 3′-diaminobenzidine (Leica).
-
bioRxiv - Developmental Biology 2024Quote: ... PFA-fixed and paraffin embedded tissue slides (5 µm) were incubated for 1h at 60°C with Bond Dewax solution (Leica Bond, AR9222) to deparaffinize tissue sections ...
-
bioRxiv - Neuroscience 2024Quote: ... Frozen sections were immersed in 0.5% uranyl acetate in methanol at –90°C in the AFS freeze-substitution unit (Leica Microsystems), infiltrated at –45°C with Lowicryl HM-20 resin (Chemische Werke Lowi ...
-
bioRxiv - Cell Biology 2024Quote: ... Frozen pellets were immersed in 0.5% uranyl acetate in methanol at −90°C in an AFS freeze-substitution unit (Leica Microsystems), infiltrated at −45°C with Lowicryl HM-20 resin (Electron Microscopy Sciences ...
-
bioRxiv - Microbiology 2021Quote: ... Cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was for 40 h at – 20°C followed by 40 h at +20°C Ultrathin-sections of Lowicryl-embedded material were collected on Formvar-carbon-coated gold grids (200 mesh ...
-
bioRxiv - Systems Biology 2022Quote: ... followed by a blocking step for endogenous peroxidase using 0.3% H2O2/methanol and fixation with 10% neutral buffered formalin (Leica Biosystems, Germany). Slides were washed in Milli-Q water and 0.05% Tween20 in 1x Tris-Buffered Saline (TBS-T) ...
-
bioRxiv - Cell Biology 2023Quote: ... cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was performed for 48 h at – 20°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... or 1:300 (rabbit anti-cleaved caspase 3, Cell Signaling Technology, 9665S; rabbit anti-p53, Leica Microsystems ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
-
bioRxiv - Physiology 2023Quote: ... and incubated for 3 x 1 h in Xylene in an automatized system (TP1020 Tissue Processor, Leica Biosystems). Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems) ...
-
bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
-
bioRxiv - Cancer Biology 2024Quote: ... 3 spectral detectors (HyD-RLD, Leica) and a 37°C preheated 40X objective (NA 1.10 ...
-
bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
-
bioRxiv - Biochemistry 2022Quote: ... Pelco Easiglow) and vitrified (3 μL sample, 3 s blot time) using an automatic plunge freezer (Leica). EndoX-TSEN•CLP1 was frozen on R1.2/1.3 Cu 300 mesh grids (Quantifoil ...
-
bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
-
bioRxiv - Cell Biology 2021Quote: ... Micro 3 - a DMi8 microscope (Leica Microsystems) with a high-contrast Plan Apochromat 63×/1.30 Glyc CORR CS objective (Leica Microsystems) ...
-
bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
-
bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
-
bioRxiv - Developmental Biology 2021Quote: ... GV-stage oocytes were injected with 10 pl of a solution containing 0.1 mM Emi2-3’UTR-MO or Emi2-3’UTR 5mm-MO using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium ...
-
bioRxiv - Microbiology 2020Quote: ... coated with 3 nm gold/palladium (Leica CPD300) and examined with a JEOL JSM-7400F scanning electron microscope (3 kV-LEI detector).
-
bioRxiv - Neuroscience 2023Quote: ... stained with 3% lead citrate (Leica UltroStain II) for 3 m ...
-
bioRxiv - Immunology 2024Quote: ... the tip of the 3-mm impactor (Leica) piston was angled and kept perpendicular to the exposed cortical surface ...
-
bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
-
bioRxiv - Bioengineering 2024Quote: ... was added to the confocal dish with a 3 mm x 3 mm hole and observed under a confocal microscope (STEDYCON, Leica, Germany).
-
bioRxiv - Neuroscience 2024Quote: ... were prepared from Sprague Dawley rats (n=3; 3-4 weeks old; Janvier Laboratories) and cut using a vibratome (Leica VT1200S) in an ice-cold oxygenated ...
-
bioRxiv - Plant Biology 2020Quote: ... Sections (3-4μm) cut by rotary microtome (Leica RM2265) were stained with 0.01 % aqueous toluidine blue ...
-
bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
-
bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
-
bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3 µm sections were cut on a microtome (Leica), rehydrated through an alcohol series according to standard laboratory procedures and then stained with a PAS staining kit (TCS Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3 µm sections were obtained with a microtome (Leica RM2255 Fully Automated Rotary Microtome) ...
-
bioRxiv - Cell Biology 2024Quote: ... and Ultrostain-2 3% Lead citrate (Leica Microsystems#16707235). Images acquisition was done with JEOL JEM-1230 transmission electron microscope ...
-
bioRxiv - Microbiology 2024Quote: ... and IBA1 using the Bond- RX/3 autostainer (Leica) with the BPRD kit (DS9800 ...