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Citations for Leica :
251 - 300 of 533 citations for 14 3 3 Zeta YWHAZ Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Molecular Biology 2019Quote: ... Retinal sections of 14 µm were cut using a cryostat (Leica CryoCut 1800), mounted on Superfrost Plus slides (Fisher) ...
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bioRxiv - Developmental Biology 2019Quote: ... and sectioned at 14 μm on a cryostat unless otherwise stated (Leica, CM3050S).
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bioRxiv - Developmental Biology 2022Quote: ... Transverse cryosections (thickness: 14 mm) were cut using a Leica CM3050S cryostat (Leica Microsystems Limited ...
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bioRxiv - Neuroscience 2020Quote: ... at 14 or 20 μm on a semi-automated rotary microtome (Leica, RM2245) and mounted on Superfrost Plus Microscope Slides in distilled H2O heated to 45°C ...
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bioRxiv - Bioengineering 2020Quote: ... Slices measuring 14 μm in thickness were sectioned using a CM1900 microtome (Leica) and mounted to glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... and 14‐μm sections were cut at –20°C on a cryostat (Leica) and collected on slides ...
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bioRxiv - Cancer Biology 2023Quote: ... Frozen tissue was sectioned to 14 μm using Cryostat Leica CM3050 S (LEICA). Frozen sections were hydrated with PBS for 10min at RT and stained using Senescence β-Galactosidase staining kit according to manufacturer’s instructions (Cell signaling technology ...
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Cancer Biology 2019Quote: ... homogeneous and mixed spheroids were generated and imaged repeatedly over time (2-3 times per week) using brightfield microscopy (4x objective; 0.3 NA, Leica DM IRBE, Hamamatsu). Spheroid volumes were calculated using SpheroidSizer58.
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
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bioRxiv - Cancer Biology 2019Quote: ... Larvae were washed 3 times with PBS 1x and livers were carefully dissected with the use of forceps on a stereomicroscope (Leica MZ 9.5). After dissection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2024Quote: Paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 30 NCs) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany) (46 ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Microbiology 2020Quote: ... for cryosectioning 14-µm sections for brains and 20-µm sections for embryos (Leica) onto slides (SuperFrost Plus ...
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bioRxiv - Neuroscience 2020Quote: ... These sections were cut at 14-16um thickness on a microtome cryostat (Leica CM1850), thaw mounted onto charged borosilicate slides (Fisherbrand Superfrost #12-550-15 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections with a thickness of 14 μm were obtained with a cryostat (Leica CM1850). Immunofluorescence was performed as follows ...
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bioRxiv - Developmental Biology 2020Quote: ... Embedded samples were cryosectioned at 14–50μm using a cryostat (Leica CM1850, Wetzlar, Germany) and adhered to positively charged glass slides (Fisherbrand ColorFrost Plus) ...
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bioRxiv - Neuroscience 2020Quote: ... Cryosections of 14-16 µm were obtained with a CM3050S cryostat (Leica, Concord, ON) in duplicate ...
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bioRxiv - Physiology 2022Quote: ... The embedded frozen blocks were cut at 14 μm thick using cryostat CM1850 (Leica) and mounted on slides ...
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bioRxiv - Physiology 2023Quote: ... Transverse sections of 14 µm diameter were obtained with a cryostat (Leica CM-1900) and deposited on poly-L-lysine-coated slides ...
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bioRxiv - Microbiology 2019Quote: Purified RNA was stained with cyanine 3 or cyanine 5 using the ULS Fluorescent Labeling Kit for Agilent arrays from Kreatech (Leica Biosystems, Amsterdam, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Immunology 2019Quote: Pools of 20 larvae were collected at 3- and 4-day post infection (dpi) and imaged by using the Leica MZ16FA Fluorescence Stereo Microscope (Leica Microsystems, Wetzlar Germany) equipped with the DFC420C color camera (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequently the samples were immersed in 100% ethanol for 3×10 min and then critical point dried in an Automated Critical Point Dryer Leica EM CPD300 (Leica Microsystems, Vienna, Austria) with 18 steps of ethanol - CO2 exchange.
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...