Labshake search
Citations for Leica :
351 - 400 of 520 citations for 10 Methylundecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... Cryosections of 10 µm thickness were cut from snap-frozen samples using a cryomicrotome (CM1950; Leica Biosystems, Wetzlar, Germany). Cryosectioned samples were used for tissue staining and stored at -20°C ...
-
bioRxiv - Neuroscience 2020Quote: ... 10–30 serial ultrathin sections (55 ± 5nm) were cut using a Leica Ultracut S ultramicrotome (Leica Microsystems, Vienna, Austria) and collected on Formvar-coated slot copper grids ...
-
bioRxiv - Molecular Biology 2022Quote: ... Frozen muscles were transversely cryosectioned into 10 µM thick sections at the mid-belly on a cryostat (Leica Biosystems). Sections were stained with haematoxylin (H&E) ...
-
bioRxiv - Neuroscience 2022Quote: ... Frozen tissues were sliced coronally into 7 - 10 μm thick sections using a cryostat machine (Leica CM3050 S, Germany), mounted on glass slides and stored at −20 °C.
-
bioRxiv - Microbiology 2023Quote: ... 10 and 24 h) using a Lumix DC T290 camera (Panasonic) equipped with a DC Vario-ELMAR lens (Leica). The area covered by bacterial cells at a given timepoint as well as the area of the whole agar plate were measured using Fiji ImageJ 1.52p ...
-
bioRxiv - Neuroscience 2023Quote: ... Z-stacks were collected with a step size of 0.2 µm and deconvolved using the LAS AF One Software Blind algorithm (10 iterations) from Leica Software v3.1.0 ...
-
bioRxiv - Plant Biology 2023Quote: ... Grids were blotted for 2.5 s at 10 °C and 95 % humidity using a Leica EM GP plunger (Leica). Data for NCPs was acquired at the Cryo-EM facility of the Gene Centre in Munich ...
-
bioRxiv - Neuroscience 2023Quote: ... 10 μm thick cryosections of the cerebral cortex and hippocampus were prepared using a vibratome (Leica VT1200, Wetzlar, Germany). The sections were Hoechst-stained ...
-
bioRxiv - Bioengineering 2023Quote: ... was performed before coating of the samples with a 10 nm gold/palladium layer (Sputter Coater EM SCD500, Leica). Scanning electron microscopy was performed in a high vacuum environment at 10 kV voltage (Quattro S ...
-
bioRxiv - Biophysics 2023Quote: Tiled images were captured using a Leica TCS SP8 laser scanning confocal microscope with a 20× oil immersion (NA 0.75) or a 10× air (NA 0.3) objective (Leica Microsystems). Images were merged and processed using LAS X (Leica Microsystems ...
-
bioRxiv - Molecular Biology 2023Quote: ... Inhibitor-induced effects on worm viability were assessed using a stereo microscope at 10× magnification (M125 C, Leica, Germany) and the following scores ...
-
bioRxiv - Developmental Biology 2021Quote: ... Then the samples were embedded in a mixture of OCT and water at a ratio of 1:1 and sectioned at 10 μm thickness with freezing microtome (Leica). The mRNA detection by in situ hybridization was performed as previously described (Lauter et al. ...
-
bioRxiv - Cell Biology 2022Quote: Freshly-extracted brains were frozen in isopentane at –80°C and then cut into 10 μm sections with a CM1950 Cryostat (Leica). After cutting ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains from rats treated with vehicle or HET0016 during the ASL experiment were dissected within 10 min after MRI and 500-μm thick live brain slices were cut using a Vibratome (Leica) spanning the sensorimotor cortex within the MCA territory ...
-
bioRxiv - Immunology 2021Quote: Kidneys were isolated from resting mice after PBS perfusion and fixed O/N in 20 mL of 10% Neutral Buffered Formalin (Leica). Fixed kidneys were paraffin-embedded using a Shandon Citadel 1000 tissue processor (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... The spheroids were then imbedded in OCT compound (Fisher Healthcare, Waltham, MA, USA) and sectioned into 10 μm thick slices using a cryostat instrument (Leica CM1950 ...
-
bioRxiv - Bioengineering 2021Quote: ... The spheroids were then imbedded in OCT compound (Fisher Healthcare, Waltham, MA, USA) and sectioned into 10 μm thick slices using a cryostat instrument (Leica CM1950; Leica Biosystems Inc. ...
-
bioRxiv - Physiology 2021Quote: Serial OCT mounted cryostat sections (10 µm in thickness) were cut at -14°C (Leica Cryostat (Fisher Supply, Waltham, MA)) ...
-
bioRxiv - Systems Biology 2021Quote: ... The samples were stored at −80°C until 10-15 μm coronal sections were cut using a cryostat (Leica CM3050). The sections were immediately placed on the functionalized coverslips described above ...
-
bioRxiv - Physiology 2021Quote: ... and serial cross-sections (10 μm) were cut using a Leica CM 3050S cryostat (Leica Microsystems, Buffalo Grove, IL, USA). For hematoxylin and eosin (H&E ...
-
bioRxiv - Cell Biology 2021Quote: ... Time lapse images were collected every 10 seconds using 63x/1.4 numerical aperture (NA) objective and LAS AF software (Leica Biosystems). Imaging was started at 20°C ...
-
bioRxiv - Neuroscience 2020Quote: ... In short: tissue blocks were sectioned at 10 μm in an RNAse free cryostat and mounted on PEN-slides (Leica). Immediately after mounting ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
-
bioRxiv - Microbiology 2022Quote: ... solani IPO 2222 Tn5 mutants was analyzed using a Leica MZ10 F stereomicroscope with 10× and 40× magnifications combined with a Leica DFC450C camera (Leica) as previously described (Lisicka et al. ...
-
bioRxiv - Biophysics 2022Quote: ... Samples were cross-sectioned into slices of 10 μm thickness by cryo-microtomy at −15°C (CM3050 S, Leica, Austria). The transversal sections were put onto microscope slides with a drop of D2O ...
-
bioRxiv - Plant Biology 2022Quote: ... The measurements were performed for a maximum time of 10 min based on a modified protocol (Glöckner et al., 2020) with a SP8 confocal laser scanning microscope (Leica Microsystems) equipped with Leica Microsystems Application Suite software and a FastFLIM upgrade from PicoQuant consisting of Sepia Multichannel Picosecond Diode Laser ...
-
bioRxiv - Plant Biology 2022Quote: ... rinsed in water and embedded in 10% agarose prior to sectioning to 50 μm with a VT1000S vibratome (Leica, Sweden). TE cell wall autofluorescence was acquired using LSM800 (Zeiss ...
-
bioRxiv - Cell Biology 2022Quote: For MALDI-MS imaging experiments 10 μm thick paw cryosections were cut with a cryostat (CM 1950 cryostat, Leica Biosystems), thaw-mounted onto conductive indium thin oxide coated glass slides (ITO ...
-
bioRxiv - Cell Biology 2019Quote: ... Single-cell tracks were acquired using live-cell imaging at 10 min intervals over 24 h (AS MDW live cell imaging system, Leica). Multiple position imaging was gathered using Image Pro 6.3 (Media Cybernetics Ltd ...
-
bioRxiv - Neuroscience 2020Quote: ... The injury section was further cut into 30 μm sections and mounted onto a series of 10 slides coated with Silane Surgipath Solution (Leica). These slides were then stored at −80° C ...
-
bioRxiv - Neuroscience 2020Quote: ... Tile-scan z stack images (10-15 optical sections depending on slice thickness) were collected using an SP8 confocal microscope (Leica) with a 20X magnification lens and Leica LASX software ...
-
bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
-
bioRxiv - Neuroscience 2019Quote: Cryosections of 10 µm thickness were cut at −20°C in a cryostat (Leica Biosystems CM3050 S Research Cryostat, Leica Biosystems Nussloch GmbH ...
-
bioRxiv - Microbiology 2019Quote: ... Several separate positions of each cell mixture were imaged every 10-15 min after auto-focusing using the LASX software package (Leica). Images were further processed with the FIJI software using the Bio-Formats plugin [61] ...
-
bioRxiv - Developmental Biology 2019Quote: ... cxcr4b:h2a-mCherry embryos with and without the hsp70l:zGrad-IRES-h2a-TagBFP transgene were heat shocked around 29-30 hpf at 39.5 °C for one hour in a water bath and imaged 80 min after the end of heat shock every 10 min for 9.5 hours on a Leica SP5 II confocal microscope equipped with HyD detectors (Leica Microsystems) using a 20x (NA 0.5 ...
-
bioRxiv - Developmental Biology 2019Quote: ... heat shocked around 31 hpf at 39.5 °C for 30 min in a water bath and imaged 35 min after the end of heat shock every 10 min for 4.8 hours on a Leica SP5 II confocal microscope equipped with HyD detectors (Leica Microsystems) using a 40x (NA 0.8 ...
-
bioRxiv - Genetics 2021Quote: ... Samples were frozen in an ethanol/dry-ice bath and 10 to 14-micron thick sections were cut with a cryostat (Leica). Sections were permeabilized and blocked for 1-hour at room temperature with 0.3% Triton X-100 and 10% normal donkey serum ...
-
bioRxiv - Cell Biology 2021Quote: ... Tissue was sectioned on an adhesive tape at 10-μm thickness using a CM1950 (w/Cryolane) cryostat (Leica, Wetzlar, Germany) at −30°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... the gap widths of scratch re-population were recorded under the microscope at 10 X magnification (Leica DMi8, Wetzlar, Germany) by measuring ten consistently sized areas per well ...
-
bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
-
bioRxiv - Cell Biology 2022Quote: ... and skeletal muscle (10 µm thickness) were cut using a Leica CM3050 S cryostat (Leica Biosystems, Buffalo Grove, IL, USA).
-
bioRxiv - Plant Biology 2022Quote: ... roots were dipped in propidium iodide (PI) solution (10 µg/ml) for 30 secs to stain the root cell wall (Leica SP8 confocal microscope ...
-
bioRxiv - Microbiology 2022Quote: ... The coverslips were fitted onto sample stubs with carbon adhesive tabs and sputter coated with 10 nm tungsten (Leica SCD500). Finally ...
-
bioRxiv - Developmental Biology 2023Quote: ... organoids were cryopreserved, embedded in OCT (Cell Path Ltd, Newtown, UK) and cut (10 µm) on a cryostat (Leica Cm1860). IF was performed as previously described [59] ...
-
bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
-
bioRxiv - Biochemistry 2023Quote: ... The fresh Phenol Red-free DMEM media with 10% FBS was added prior to confocal microscopic experiment (Leica Stellaris 8). A 405 nm laser excitation was used to image BFP and a 488 nm laser excitation was used to image EGFP.
-
bioRxiv - Cancer Biology 2023Quote: ... snap-frozen livers were embedded in Tissue-Tek® O.C.T (Sakura: 4583) and cut in 10 μm sections using Leica Cryostat (Leica Biosystems). 5 sections in a distance of 100 μm to each other were stained per liver ...
-
bioRxiv - Zoology 2023Quote: The histochemical investigation was conducted on tegumental enzymes using frozen sections prepared and cut to 10-12 µm in a cryostat (CM 3050S, Leica). The modified lead nitrate method detected acid phosphatase (AcPase ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
-
bioRxiv - Neuroscience 2023Quote: ... Images were captured at 10-s intervals for 12 minutes by using a confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The cross-sectional area of central canal was normalized to the baseline area in 0 minute ...