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Citations for Leica :
401 - 450 of 557 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the block was trimmed at a depth of 100 µm and a 45° angle on four sides using a trimming machine (EM TRIM, Leica). Serial sections were taken with a 35° ultra-maxi diamond knife (Diatome ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
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bioRxiv - Bioengineering 2023Quote: A representative brain from a mouse stimulated with DCZ (mouse 4N with the cranial window) was sectioned into 100 µm slices using a vibrating microtome (Leica). The slices were mounted and stained with DAAPI nuclear stain using ProLong Diamond antifade mountant with DAPI (Thermo Fisher P36962 ...
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bioRxiv - Neuroscience 2023Quote: STED images were acquired from fixed samples by a HC PL APO CS2 100 x oil objective (1.40 NA) on a Leica SP8 TCS STED 3x microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Cell Biology 2023Quote: ... 0.3 mm/flat) (Technotrade 1191-100) was placed on top of the specimen carrier A and a 200 µm spacer ring (Leica) was placed on top ...
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bioRxiv - Cell Biology 2023Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Microbiology 2023Quote: ... were cleaned with ethanol (100%) and coated with a 15 nm carbon layer using a sputter coater (EM ACE600, Leica). To distinguish between the two sides of the disc ...
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned at 50 µm (acvrl1) or 100 µm (cdh5) with a VT1000S vibratome (Leica Microsystems, Buffalo Grove, IL, USA). We mounted sections with Vectashield mounting media and captured brightfield images using an Olympus BX51 upright compound microscope outfitted with a UPAN FLN 20X/0.5 NA dry objective and DP71 camera as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Serial coronal sections were cut at 100-μm thickness with a vibratome (Leica VT 1200; Leica Biosystems, Buffalo Grove, IL) starting at +3.8 mm Bregma and ending at −6.50 mm Bregma ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
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bioRxiv - Neuroscience 2024Quote: ... The brains were washed three times in PBS and sectioned (75-100 μm thickness) in a VT1000 Vibratome (Leica Biosystems). Tissue sections were incubated in antigen retrieval solution (10 mM sodium citrate ...
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bioRxiv - Paleontology 2023Quote: ... put flat under a Leica Map DCM8 profilometer (TRIDENT, PALEVOPRIM Poitiers) and scanned using white light confocal technology with a 100× objective (Leica Microsystems ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were embedded within a 3% agarose (BP160-100, Fisher Bioreagents) mixture and sectioned in a vibratome (VT 1200, Leica) into 300-µm thick sections ...
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bioRxiv - Biophysics 2024Quote: ... were evenly positioned on a ring installed 5 cm above the sample positioned at the focal plane of the objective lens (OBJ; 100×/1.44, Leica, Germany), as shown in Fig ...
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bioRxiv - Cell Biology 2024Quote: ... and QWF-T (size P)) using a 100× Plan Apo 1.47 NA oil-immersion TIRF objective (Leica Microsystems, Wetzlar, Germany). Images were captured in 5 s intervals for 20 min (unless otherwise noted ...
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bioRxiv - Cell Biology 2023Quote: ... Z-stacks were recorded every 200 nm with a 100 x oil objective and sCMOS camera (DFC9000) equipped on Thunder Imaging System (Leica). Deconvolution and kinetochore protein quantification were performed with LAS X software (Leica).
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bioRxiv - Neuroscience 2024Quote: ... the tissue was sliced in 50 μm-thick (for brainstem) or 100 μm-thick (for thalamus or cortex) sections using either a vibratome (Microtome Leica VT1000 S ...
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bioRxiv - Cancer Biology 2021Quote: ... ECs were dissected and catapulted into the cap of 0.5 mL tubes (Leica) after removal of blood cells from capillaries ...
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bioRxiv - Cell Biology 2019Quote: ... the specimen carrier sandwich was transferred to the middle plate of an HPM 100 high-pressure freezer (Leica Microsystems, Vienna, Austria) and frozen immediately without using ethanol as synchronizing medium.
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bioRxiv - Cell Biology 2022Quote: ... Live-cell imaging was performed at 37°C with a Leica TCS SP8 confocal microscope with a 100×/1.40 Oil HC PL APO CS2 objective (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Cell Biology 2022Quote: ... Live-cell imaging was performed using Leica TCS SP8 confocal microscope with a 100×/1.40 Oil HC PL APO CS2 objective (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Neuroscience 2020Quote: ... was introduced at an impact depth of 0.5 mm with a 2-mm diameter round impact tip (3.0 m/s speed and 100 ms dwell time) by using an electromagnetically driven CCI device (Impact One™ Stereotaxic CCI Instrument, Leica). After injury ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were then sectioned at a thickness of 100 μm while frozen on a sliding microtome (Leica SM2010 R, Nussloch, Germany). Brain sections were mounted on 1×3 in ...
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bioRxiv - Biophysics 2019Quote: Our setup is based on a DMI300B or DMI8 microscope with an oil immersion HCX PL APO 100×/1.47 NA TIRF objective (Leica, Manheim, Germany). We used an Evolve 512 EMCCD camera (Photometrics ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed brains were sectioned into 100 μm sections in the coronal plane using a Leica VT-1000 vibrating microtome (Leica Microsystems) and stored in PBS until later analysis ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2020Quote: ... Coronal (100 μm thick) and sagittal (60 μm thick) sections were made using Leica VT1200 S vibratome (Leica Microsystems, Nussloch, Germany) and collected in PBS containing 0.01% sodium azide and stored at 4°C until use ...
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bioRxiv - Microbiology 2021Quote: ... collecting ∼200 000 bright-field images at 5 ms resolution with a 100× oil objective on a Leica DMi8 inverted microscope (Leica, Germany) at a set temperature value of 30°C.
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bioRxiv - Cell Biology 2021Quote: ... around 1.5 µl of concentrated purified schizont pellet was transferred into aluminium or gold carriers (3 mm diameter, 100 or 200 µm depth; Leica Microsystems) and high pressure frozen with EM ice (Leica Microsystems) ...
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Cell Biology 2020Quote: ... either a Leica Objective HC APO CS2 100×/1.40 Oil or a Glycerol objective (Leica, HC APO 93x/1.30 GLYC motCORR) were used.
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bioRxiv - Neuroscience 2020Quote: ... Fixed brains were sectioned into 100 μm thick sections in the coronal plane using a Leica VT-1000 vibrating microtome (Leica Microsystems). Embryonic brain tissues were dissected at specific post-gestation dates ...
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bioRxiv - Microbiology 2021Quote: ... was transferred to the freezing container (specimen carriers type A, 100 μm, covered with specimen carriers type B, 0 μm, Leica Microsystems). High pressure freezing was done in an EM HPM100 (Leica Microsystems ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Bioengineering 2019Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm and 100 ms impact duration (Impact ONE; Leica Microsystems). The skin was sutured and the animals were placed in a 37°C incubator until consciousness was regained ...
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bioRxiv - Cell Biology 2019Quote: ... pelleted by vacuum-filtration and high-pressure frozen in the 100 µm recess of aluminium platelets (Wohlwend) using an HPM100 (Leica Microsystems). Samples were freeze-substituted and embedded in Lowicryl HM20 (Polysciences ...
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bioRxiv - Cell Biology 2021Quote: ... the specimen carrier sandwich was transferred to the middle plate of an HPM 100 high-pressure freezer (Leica Microsystems, Vienna, Austria) and frozen immediately without using ethanol as synchronizing medium ...
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bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
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bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software). Images were acquired as z-stacks with a z-step size of 120 nm and a X-Y resolution of 37.9 nm ...
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bioRxiv - Cell Biology 2021Quote: ... microscopy was performed with the 100×/1.4 Oil HC PL APO CS2 STED White objective of a Leica TCS SP8 3× STED microscope (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Neuroscience 2022Quote: ... carbogenated dissecting aCSF and 300 μm transverse slices made with a cutting speed of 100 μm/s on a Leica Vibratome (Leica, CT1200). Slices were transferred to a recovery chamber filled with carbogenated pre-warmed (35 degrees Celsius ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed brains were sectioned into 100 μm sections in the coronal plane using a Leica VT-1000 vibrating microtome (Leica Microsystems) and stored in antigen preserved solution (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... were separated by a 100 μm stainless steel spacer (Technotrade international 1257-100) and processed for high pressure freezing on a Leica EM ICE high pressure freezer (Leica microsystems). Following high pressure freezing ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were then washed in PBS and sectioned along the coronal plane at 100 µm using a vibrating microtome (Leica VT1200S). Sections were collected spanning the approximate bounds of the somatosensory cortex ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...