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Citations for Leica :
401 - 450 of 582 citations for L Serine 1 13C 99%; 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... z-stack images (z-step intervals of 1 μm) were sequentially acquired using a 63X/1.4 oil objective and a DFC365 FX CCD Camera (Leica) with a x-y sampling of 72 nm ...
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bioRxiv - Biophysics 2021Quote: ... and incubated for 10 s prior to blotting on Whatman 1 paper for 4 s and plunge-frozen in liquid ethane using a GP2 plunge freezer (Leica) equilibrated to 10°C and 85% humidity ...
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bioRxiv - Cell Biology 2020Quote: Imaging data for the under agarose assay 1 were collected using a Leica DMI-4000B inverted microscope (Leica Microsystems Inc.) mounted on a TMC isolation platform (Technical Manufacturing Corporation ...
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bioRxiv - Developmental Biology 2021Quote: ... and the preparations were cleared in a series of increasing (20 to 80%) glycerol/1% KOH ratio until storage in 100% glycerin and photography (Leica MZ6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Worms were freeze substituted for 2 h at −90 °C with 1% osmium tetroxide and 0.1% uranyl acetate in acetone using a Leica EM AFS2 freeze substitution processor (Leica Microsystems). After a temperature ramp to 0°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... cell monolayers were ultrathin sectioned at 80 nm on formvar-coated copper grids (Plano) and postcontrasted using 1 % uranyl acetate and ultrostain (Leica). TEM images for Fig ...
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bioRxiv - Cell Biology 2019Quote: PFA-fixed adult mouse (3-4 month) brains were cut into 1-mm-thick sections using a vibratome (Leica, VT1200S) [11 ...
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bioRxiv - Neuroscience 2019Quote: ... All brightfield images included in Supplementary Figure 1 were acquired using a HC PL APO 63×1.40 oil objective using a Leica DFC450 digital camera (Leica Microsystems).
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bioRxiv - Cancer Biology 2019Quote: ... Sections were stained using a Leica BOND-MAX-TM autostainer with BondTM epitope retrieval 1 or 2 and the Bond Refine Detection Kit (Leica). Primary antibodies are listed in Supplementary Table S1.
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Neuroscience 2020Quote: ... and EB3 comets of a single cell were imaged for 120 seconds (1 frame per second) with a Leica DMi8 microscope (Leica). Original movie file was processed by ImageJ software (Schindelin et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was finally washed with PBS-T for 1 day before being imaged on a TCS SP5 confocal microscope (Leica).
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bioRxiv - Biophysics 2022Quote: ... was mixed quickly with the ATP solution on the grid before blotting for 1 s in an EM GP2 grid freezing device (Leica) at 4 °C and 100% humidity and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were stretched with chloroform to eliminate compression and mounted on Pioloform filmed copper grids prior to staining with 1% aqueous uranyl acetate and lead citrate (Leica). They were viewed on a Philips CM100 Compustage Transmission Electron Microscope with images collected using an AMT CCD camera (Deben) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA/DNA FISH) were acquired with a laser-scanning confocal microscope with a pinhole diameter of 1 Airy unit (Leica TCS SP5 or SP8 ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) (. Cells were imaged using a Leica DMi8 confocal microscope (63x ...
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Social Regulation of Egg Size Plasticity in the Honey Bee is Mediated by Cytoskeleton Organizer Rho1bioRxiv - Evolutionary Biology 2022Quote: ... The dehydrated samples were embedded in paraffin and then cut into 1 μm sections using a RM2235 microtome (Leica, Germany) that were gently deposited onto glass microscope slides ...
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bioRxiv - Immunology 2022Quote: ... sections were stained with hematoxylin for 1 minute followed by whole slide imaging using an Aperio ImageScope AT (Leica Biosystems) at 20X magnification ...
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bioRxiv - Cancer Biology 2022Quote: ... Heat-induced epitope retrieval was performed using citrate-based BOND Epitope Retrieval Solution 1 (Leica Biosystems, Buffalo Grove, IL, USA) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections (50nm) were deposited onto formvar-coated copper grids (Plano) and postcontrasted using 1% uranyl acetate in water and ultrostain (Leica). TEM images were acquired on a JEM 1400plus (JEOL ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in dichloromethane and then transferred to a clearing solution (benzyl alcohol: benzyl benzoate (1:2)) and imaged with a confocal laser scanning microscope (SP8, Leica).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IHC at a 1:50 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine RED Kit (Leica) minus Hematoxylin.
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bioRxiv - Biophysics 2020Quote: ... deeply anesthetized fish larvae with immobilized hearts were embedded in 1% low-melting agarose for sustained confocal imaging (SP8-STED/FLIM/FCS, Leica) using a 20×/0.75 Objective (HC PL APO CS2) ...
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bioRxiv - Neuroscience 2019Quote: ... C57BL/6J pups were cut into 350-μm thick sections in ice-cold Gey’s balanced salt solution (GBSS) supplemented with the glutamate receptor antagonist kynurenic acid (1 mM) and glucose with a vibratome (Leica). Slices with intact morphology were collected into the same GBSS solution and kept on ice ...
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bioRxiv - Immunology 2021Quote: ... were cut and deposited onto formvar-coated grids (Plano) and again contrasted using 1 % uranyl acetate in water and Ultrostain (Leica). Images were acquired on a JEM 1400plus (JEOL).
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μm thick sections were cut with a glass knife from each resin-embedded tissue block on an ultramicrotome (Leica EM UC6 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were washed with PBS containing 1% bovine serum albumin and the fluorescence (red and blue) was imaged under a fluorescence microscope (Leica). At least three fields of view were selected and analyzed using ImageJ software.
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bioRxiv - Microbiology 2022Quote: ... Cells were kept in serum- and phenol red-free MEM containing 1% glucose during the analysis (Leica Biosystems 6000B microscope). A total of 15 random snapshots (55-70 cells in total ...
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bioRxiv - Immunology 2022Quote: Imaging was performed on an inverted Leica TCS SP8 confocal microscope with white light laser and HyD photodetectors using an HC PL APO CS2 20 × /0.75 IMM objective (zoom factor of 1) (Leica Microsystems). Images were deconvolved using the LIGHTNING module in Leica Application Suite X (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cell Biology 2022Quote: ... the pulp chambers of the mandibular first molars were opened by a high-speed handpiece with #1/4 carbide round bur under direct visualization by a stereoscopic microscope (Leica). A #10 endodontic K file was then used to probe the root canals ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings with the typical mdf-1 phenotype were transferred to a new plate to be observed under the fluorescent stereo microscope (Leica M165 FC Fluorescent stereo microscope equipped with a Leica DFC 420C camera ...
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Molecular Biology 2022Quote: ... Adult animals were then immobilized with 1% sodium azide and GFP fluorescence was assessed using a DM6000B-Cs microscope (Leica) equipped with a DFC 365FX camera (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... and stained with 0.1% toluidine blue to quantify the different germ cell types at 100x objective using a high-resolution light microscope (Leica DM6000 BD ...
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bioRxiv - Biophysics 2023Quote: ... The collagen gel is stretched uniaxially in 1% step increments using a custom made stepper motor-driven cell stretching device (46) mounted on an epifluorescence microscope (Leica DMI6000 CS equipped with a 20x Leica HCX PL Fluotator objective ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Pathology 2023Quote: ... semi-thin sections measuring 1 µm were meticulously sliced from the resin blocks employing a Reichert Ultracut S microtome (Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µm per z-step) of neurons expressing fluorescent membrane markers were captured using a 20×0.7 NA (Leica SP5) 20×0.75 NA (Leica Stellaris ...
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bioRxiv - Immunology 2023Quote: ... The emission wavelength was tuned to 841 nm and the fluorescent signal was collected using a water immersion objective (25x, NA 1, Leica) and detected with external ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
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bioRxiv - Biochemistry 2023Quote: ... In the first channel, a 1-ml syringe tip (Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) and the reference tube (Ismatec Reglo ...
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bioRxiv - Immunology 2023Quote: ... images were acquired with z-stacks between 10-15 pm (z-steps: 1 pm) and 3D-reconstructed and displayed in ‘Maximum’-mode using the Leica Application Suite X software (Leica).
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...