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Citations for Leica :
401 - 450 of 2031 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Imaging of neuronal cultures was carried out as well using confocal Leica SP8 White Light Laser 2 (Leica Microsystems, Mannheim, Germany) and with a x20 (NA 0.75 ...
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bioRxiv - Microbiology 2024Quote: ... the vitrified pellets were freeze-substituted at -90°C for 80 h in acetone supplemented with 2 % OsO4 (AFS2; Leica Microsystems). The samples temperature was increased slowly to -60°C (2°C⋅h-1) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 2 min ...
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bioRxiv - Neuroscience 2024Quote: ... Horizontal slices (300 µm) of the olfactory bulb were cut using a vibratome (VT1000S, Leica and 7000 smz-2, Campden Instruments) and maintained in artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal slices containing the mPFC (350 μm thickness) were cut at 2 °C using a Vibratome 1000 Plus (Leica Microsystems, USA) in a NMDG-containing cutting solution (in mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were subjected to heat-based antigen retrieval for 30 minutes using a high pH buffer solution (Bond Epitope Retrieval Solution 2; Leica, AR9640). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2024Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Cell Biology 2024Quote: Isolated islets were kept in culture overnight and frozen with a Leica EMpact 2 or EM ICE high-pressure freezer (Leica Microsystems). They were kept in liquid nitrogen until freeze substitution ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Bioengineering 2024Quote: ... GE-caveospheres were added to R2/2 Quantifoil grids and plunge-frozen using a Leica Electron Microscopy Grid Plunger (Leica Microsystems). Grids were imaged on a CryoARM300 (JEOL ...
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bioRxiv - Microbiology 2020Quote: The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
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bioRxiv - Physiology 2023Quote: ... and incubated for 3 x 1 h in Xylene in an automatized system (TP1020 Tissue Processor, Leica Biosystems). Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: Unstained sections from the study cases were heated for 30 minutes in Bond™ Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems) using Leica BOND-MAXTM (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was blocked in 2% agar and 200 µm thick coronal sections were cut using a vibratome (Leica VT 1000 S). The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... GFP fluorescence resulting from LanB2:GFP expression in Dilp2 neurons was imaged with a Leica TCS SP8 MP 2-photon microscope (Leica, Wetzlar, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and acute horizontal slices (310 μm thick) were prepared using a vibratome (5000mz-2, Campden Instruments Ltd.; or VT1200S, Leica Biosystems). Slices recovered for 30 min in ∼37°C oxygenated Ringer’s solution that was identical to the dissection solution except with lower Mg2+ concentrations (1 mM MgSO4 ...
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bioRxiv - Cell Biology 2021Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+ fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software (US ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Histological analysis of the 2 µm paraffin-embedded tissue sections was performed with the Bond Polymer Refine Detection Kit (Leica, Wetzlar, Germany) on a BondMax system (Leica) ...
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bioRxiv - Neuroscience 2021Quote: ... the cochlea was glued on a metal block with Loctite 401 and cut to 2 halves by a vibratome (VT1200S, Leica, Wetzlar, Germany) with FREQ index at 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Bioengineering 2022Quote: Following embedding within 2% agarose and fixation overnight samples were dehydrated and embedded in paraffin using an automatic tissue processor (ASP300; Leica, London, UK). All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal brain sections (20 μm) were cut around the bregma 0 to -2 mm using a Leica CM1950 cryostat (Leica Biosystems, Germany). For P2X7R immunofluorescence labelling ...
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bioRxiv - Physiology 2023Quote: ... the worms were randomly chosen and treated with 10 mM sodium azide in M9 solution and aligned on an 2% agarose pad on slides and images acquired using a confocal microscope (Leica TCS SPE) with a 63× objective lens ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Bioengineering 2023Quote: ... Lateral and longitudinal cross-sections of the samples were imaged after 7 days of culture in EGM-2 BulletKit medium using a Thunder imaging system (Leica Microsystems, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... the brains were removed and sectioned into 150 µm transverse slices after embedding in 2% low-melting agarose using a vibratome VT1000 (Leica, Wetzlar, Germany). The OTCs were cultured on Millicell cell cultures inserts in 6-well plates using 1 mL of FCS-free medium consisting of DMEM/F12 supplied with 1 x B27 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut, Leica Microsystems, Bensheim, Germany). The ultra-thin sections were post-contrasted in a LEICA EM STAIN (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... Images from the same area of the CA1 region of each hippocampus were acquired using a Leica DMLB 2 microscope (Leica, Wetzlar, Germany) connected to a Leica DFC320 FireWire digital microscope camera (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... Confocal z-stacks covering the whole depth of the neuron somata were taken with 0.38 μm step size using a 63× oil objective with 2× digital zoom magnification (Leica TCS SPE, Germany). Stacks were then processed using FIJI/ImageJ software ...
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bioRxiv - Neuroscience 2024Quote: ... Selected sections were also studied on a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) or scanned with an Olympus VS200 Research Slide Scanner (Olympus / Evident ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+-fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software for examining Ca2+ fluctuation amplitude and frequency ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and cut into coronal slices (thickness 300 µm, -1.5 mm to 2 mm relative to bregma) on a vibratome (Leica VT1200S, Leica Biosystems, Nussloch, Germany) in ice-cooled artificial cerebrospinal fluid (aCSF) ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then embedded in 2 % agar in PBS and cut into sagittal sections with a thickness of 50 µm using a microslicer (VT1000S, Leica, Wetzlar, Germany). The serial sections were cover-slipped with mounting medium (DAPI Fluoromount-G ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then embedded in 2 % agar in PBS and cut into sagittal sections with a thickness of 50 µm using a microslicer (VT1000S, Leica, Wetzlar, Germany). The serial sections were cover-slipped with mounting medium (DAPI Fluoromount-G ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections (∼2 x 2 x 2cm) not needed for pathologic evaluation were fixed in 10% formalin and sliced with a vibratome (Leica VT 1200S) to a thickness of 2mm ...
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bioRxiv - Neuroscience 2023Quote: ... Stellaris 5 (Leica), or LSM 900 (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 spectral detectors (HyD-RLD, Leica) and a 37°C preheated 40X objective (NA 1.10 ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
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bioRxiv - Cancer Biology 2022Quote: ... brains were submerged in ice-cold and oxygenated media (DMEM High-Glucose without phenol red, GibcoTM, USA) and sectioned in a Leica VT100S vibratome (Leica, Buffalo Grove, IL) set to 300 μm in the z-direction ...
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bioRxiv - Biophysics 2022Quote: ... Embedded brains were then immersed in ice-cold and oxygenated DMEM media without phenol red and then sectioned in a Leica VT100S vibratome (Leica, Buffalo Grove, IL). 300 mm thick brain tumor sections were transferred to laminin coated cell culture insert (Millipore Sigma ...