Labshake search
Citations for Leica :
351 - 400 of 499 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Bright-field time-lapse imaging was performed on an inverted microscope Leica DMI 6000B (Leica Microsystems, Germany) in a controlled environment (37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse imaging of cells spreading on the pillars was performed using an inverted microscope (Leica DMIRE2) at 37°C using a 63x 1.4 NA oil immersion objective ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were washed with ethanol three times and cut with a vibratome (Leica VT1000ST, Leica, Germany) at 50 µm thickness and stored in 100% ethanol at -20°C until further use.
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bioRxiv - Neuroscience 2022Quote: ... The samples were washed with ethanol three times and cut with a vibratome (Leica VT1000ST, Leica, Germany) at 50 µm thickness and stored in 100% ethanol at -20°C until further use.
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bioRxiv - Cancer Biology 2023Quote: ... Images were captured at two-time points (0 and 48 hrs) using a phase-contrast microscope (LEICA) to analyze the morphological effect of the compound on cells.
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bioRxiv - Cancer Biology 2023Quote: ... and monitored using a 40x objective on a DeltaVision time-lapse microscope (Applied Precision/GE Healthcare/Leica). Time-lapse data were analysed using SoftWorx (Applied Preciesion ...
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bioRxiv - Developmental Biology 2024Quote: All experiments of time-lapse microscopy of growing embryos were recorded under a bright-field microscope (Leica DMI600 B ...
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bioRxiv - Cancer Biology 2021Quote: ... was done on sections of FFPE tissue using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA), with the following antibodies and conditions (dilution ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on sections of formalin-fixed paraffin-embedded patient tissue (FFPE) using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA). Slides were de-paraffinized and heat-mediated antigen retrieval using the Bond Epitope Retrieval 1 solution at pH6 (H1 ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescence photons were detected with hybrid detectors (495 nm – 545 nm detection range) in photon counting mode using a TCSPC approach operated by the FALCON module (Leica Microsystems, Manheim, Germany) integrated within the Leica SP8 SMD system ...
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bioRxiv - Physiology 2020Quote: ... Two-four μm thickness sections from paraffin embedded samples were used for immunohistochemistry at the Tumour Bank of the HCB-IDIBAPS Biobank (IDIBAPS, Barcelona) using the Leica Microsystems’ Bond-max™ automated immunostainer together with the Bond Polymer Refine Detection System (Leica Microsystems, Spain). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The in situ hybridization experiments for the detection of CART and Pitx2 mRNA expression in spinal cord cross sections (Leica CM3050 S cryostat) were performed as described in 69.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Glial fibrillary acid protein (GFAP) Iimmunohistochemistry was carried out using GFAP primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously (39–42).
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bioRxiv - Neuroscience 2023Quote: Neuron specific enolase (NSE) immunohistochemistry was carried out using NSE primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously [54 ...
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bioRxiv - Cancer Biology 2024Quote: ... was performed on sections of formalin-fixed paraffin-embedded organoids (FFPE) using a Bond automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA) by the Translational Research Unit (TRU ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2019Quote: ... were high-pressure frozen as described using an either an EM PACT2+RTS or an EM ICE high-pressure freezer (Leica Microsystems, Vienna, Austria)(Muller-Reichert et al. ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were taken and fixed in 4% PFA at RT overnight and 50 μm slices were cut with a vibratome (Leica VT 1000 S). For brain sections from the anterograde and trans-synaptic tracing studies ...
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bioRxiv - Developmental Biology 2021Quote: ... The time-lapse movies were taken on a Leica SP8 confocal microscope equipped with HyD detectors (Leica Microsystems) using a 20x (NA 0.5 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A series of time laps pictures was taken (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD) every 10s for 24h ...
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bioRxiv - Molecular Biology 2022Quote: ... Leica SP8 FALCON FLIM with a time-correlated single photon counting (TCSPC) module was used (Leica microsystem, Germany). A pulsed ...
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bioRxiv - Neuroscience 2022Quote: ... Individual Ca2+ events were acquired with dynamic fluorescence intensity (FI) according to each time point ROI from Leica confocal microscope ...
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bioRxiv - Neuroscience 2020Quote: ... Time-lapse live imaging was performed using an upright confocal laser scanning microscope (TCS SP2 VIS/405, Leica) with a 20X HCX APO L water-dipping objective (NA 0.5) ...
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bioRxiv - Developmental Biology 2021Quote: ... Time-lapse imaging was carried out between E13.5 and E14.5 using an inverted SP8 confocal microscope (Leica microsystems), an inverted Dragonfly Spinning disc confocal microscope (Andor ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Genetics 2023Quote: ... TIRF (HILO) fluorescence time-lapse microscopy was performed on a Leica DMi8 microscope with a sCMOS DFC9000 (Leica) camera using a 100x oil-immersion Plan APO TIRF objective ...
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bioRxiv - Neuroscience 2022Quote: ... while the remaining sections were selected for immunohistochemistry procedures using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL). Immunohistochemistry was performed for glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: In situ hybridization for detection of the EBV-encoded small RNAs (EBER) was performed using the EBER Probe (Catalogue no: ISH5687; Leica Biosystems Newcastle Ltd, UK). The technique was carried out on an automated stainer ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using Leica Bond III automated staining system with diaminobenzidine chromogen detection and the same antibodies as for clinical diagnostics (DS9800, Leica Biosystems, Buffalo Grove, IL). Hematoxylin and eosin stain was used for general histopathology ...
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bioRxiv - Developmental Biology 2021Quote: ... Heart tissues fixed in 10% formalin and paraffin-embedded were sliced into serial 5-µm sections using a Leica RM2125 RTS microtome (Leica Camera AG, Wetzlar, Germany). Sections on slides were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cancer Biology 2021Quote: ... The double staining was done sequentially on a Leica Bond-IIITM instrument using the Bond Polymer Refine DAB Detection System (Leica Microsystems DS9800, Buffalo Grove, IL) and Refine Red Detection System (Leica Microsystems DS9390) ...
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bioRxiv - Neuroscience 2020Quote: ... which shows expression in uninjured axons.25 All immunostains were performed using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope by a senior pathologist (T.J.H.) ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Cancer Biology 2023Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope (TH) ...
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bioRxiv - Plant Biology 2019Quote: ... sections were washed three times with sterile water and observed under a bright-field lighting microscope (Leica DM2000 LED). At least 10 plants/genotype and 20 sections/plant were evaluated.
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bioRxiv - Immunology 2019Quote: ... Time-domain FLIM experiments were performed using a TCSPC approach operated by the FALCON module (Leica Microsystems, Manheim, Germany) integrated within the Leica SP8-X-SMD microscope ...
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bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
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bioRxiv - Developmental Biology 2019Quote: ... at which time point the localisation of the fluorescent cells was analysed using a fluorescent microscope (Leica M205 FA). In a typical experiment ...
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bioRxiv - Developmental Biology 2023Quote: The number of skeletal components and the length of pectoral fin primordia were measured at specific time points during the developmental process using col2a1a:EGFP fluorescence and Leica Application Suite X (LAS X, Leica). Differences in the number of skeletal elements and the length of between mutant and WT zebrafish were tested using an unpaired t-test using GraphPad Prism (Graphpad Software).
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bioRxiv - Cell Biology 2022Quote: ... a Leica HyD SMD time-gated photomultiplier tube and a Leica 100x (NA 1.4) STED White objective (Leica Microsystems, Inc.). ATTO 647 and Alexa 594 were excited at 647nm and 575nm with fluorescence emission collected over a bandwidth of 658–755nm and 583–700nm respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg/mL IP-10 were added on the other side of the slice and images were then acquired in time-lapse model with a SP5 confocal system (Leica) every 3 min for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: Time-lapse imaging was performed using a Leica SP5 confocal microscope with the Leica 20x 0.7NA dry lens (Leica, 506513). Seven adjacent 3.7 nm z-stacks were acquired at multiple positions for GFP (488nm excitation with Ar laser ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and at different time points following 17 mM glucose stimulation using a Leica TCS SP5 confocal microscopy and LAS X software (Leica,). FRET indexes per cell and time point were obtained by analyzing images with the ImageJ Plug-in “FRET and co-localization analyzer” (rsb.info.nih.gov/ij/plugins/fret-analyzer.html).
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bioRxiv - Immunology 2022Quote: ... were acquired with two times of frame averaging and maximum projection was then performed on using LAS X software (Leica).