Labshake search
Citations for Leica :
351 - 400 of 1711 citations for Acetamide N 2 3 dihydro 2 3 hydroxy 2 quinolinyl 1 3 dioxo 1H inden 5 yl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with a mixture of oxygen (1L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
-
bioRxiv - Physiology 2020Quote: ... Finally, sections were incubated with DAB (3,3’-diaminobenzidine, Vector) for 3 min and counterstained with Gill’s hematoxylin (Leica, Buffalo Grove, IL) for 30 sec ...
-
bioRxiv - Microbiology 2021Quote: ... containing defined regions of the bacterial colonies were cut out and inverted on lecithin-coated 3-mm specimen carriers for high pressure freezing in a high- pressure freezing machine (Leica, ICE) with or without 10% polyvinylpyrrolidone as a filler (Δeps biofilms and one of the WT samples were frozen without filler) ...
-
bioRxiv - Cell Biology 2021Quote: ... microscopy was performed with the 100×/1.4 Oil HC PL APO CS2 STED White objective of a Leica TCS SP8 3× STED microscope (Leica, Wetzlar, Germany) equipped with a white light laser ...
-
bioRxiv - Cell Biology 2023Quote: ... The paraffin embedded clots were cut in 3 µm thick sections with a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany) and placed on silanized glass slides ...
-
bioRxiv - Neuroscience 2023Quote: ... cluster of differentiation 3 (CD3) and CD68 immunostaining was performed using a fully automated staining system (BOND-III; Leica Biosystems, UK). In brief ...
-
bioRxiv - Plant Biology 2022Quote: ... were infiltrated in Nicotiana benthamiana leaves for transient expression and viewed after 3 days under confocal microscope Leica TCS SP5 (Leica Microsystems) equipped with appropriate lasers (514 nm -527 nm and 532 nm-588 nm for YFP and RFP ...
-
bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
-
bioRxiv - Molecular Biology 2023Quote: ... Image acquisition was performed as z-stack images of 250 μm in depth in 3 separate channels using a Leica SP8 confocal microscope (Leica, Germany).
-
bioRxiv - Biophysics 2023Quote: ... and then glued onto Leit-tabs (Plano, Germany) mounted on aluminum stubs (Plano, Germany) and sputter-coated with a 3 nm thin gold layer (ACD600, Leica, Austria). SEM images were acquired with a Zeiss Auriga FEG-SEM (Zeiss ...
-
bioRxiv - Neuroscience 2023Quote: ... Coronal sections of 3 µm thickness were cut at the level of the hippocampus using a microtome (Leica Microsystems, Wetzlar, Germany). For immunohistochemistry either paraffin sections that were washed with 0.5% sodium azide (2 × 5 min ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 1h incubation in the same buffer containing the primary probes and a 3 min heat incubation step in a ThermoBrite slide hybridization system (Leica Biosystems), either at 75°C or 86°C ...
-
bioRxiv - Cell Biology 2024Quote: ... was transferred to B-type planchettes (3 mm) and rapidly frozen with a Leica EM ICE high-pressure freezer (Leica Microsystems). The frozen specimens were freeze-substituted in anhydrous acetone containing 2% osmium tetroxide (OsO4 ...
-
bioRxiv - Neuroscience 2024Quote: ... IHCs: 3 µm z-resolution) from the SGNs and IHCs were taken using a Leica SP8 confocal microscope (Leica microsystems, Germany) with a 40x objective ...
-
bioRxiv - Microbiology 2024Quote: ... Images of single optical sections of about 0.5 μm thickness were captured with a confocal microscope and a 20x or 63x objective (Leica inverted 3).
-
bioRxiv - Microbiology 2024Quote: ... Cellular fluorescence signals in plant leaves agroinfiltrated with BiFC and other constructs expressing fluorescent proteins were observed at 3 dpi using a Leica SP8 laser-scanning confocal microscope (Leica, Germany) equipped with specific laser/filter combinations for YFP (excitation at 514 nm) ...
-
bioRxiv - Neuroscience 2024Quote: ... brains were washed 3 times in PBS1X and sliced into coronal sections (50 µm) using a vibrating microtome (Leica Microsystems; VT1000S). Floating slices were maintained in PBS with azide at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were washed 3 times with PBS and imaged using a Leica SP8 laser scanning confocal microscope (Leica Microsystems, Heidelberg, Germany) using a 63x water objective ...
-
bioRxiv - Cancer Biology 2024Quote: ... Immunohistochemical (IHC) staining for Estrogen receptor (ER) was conducted using the Leica Bond-3 automated stainer platform (Leica, Buffalo Grove, IL). Following antigen retrieval using a citrate-based pH 6 epitope retrieval solution ...
-
bioRxiv - Neuroscience 2024Quote: ... the sucrose solution was removed and organoids were placed on OCT (at least 3 organoids on each OCT drop) and cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 20 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Wound closure was measured at regular intervals (0-hour, 3-hour, 6-hour, 9-hour) using an inverted microscope (Leica DMi1). The wound closure distances at different time points were normalized to the distance at 0hour to calculate the migration rate.
-
bioRxiv - Neuroscience 2021Quote: ... contusion was induced using a 2 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 1.25 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
-
bioRxiv - Plant Biology 2021Quote: ... 9.3% water in water-free acetone in a Leica AFS 2 (Leica Microsystems, Wetzlar, Germany) as described previously (Flechsler et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
-
bioRxiv - Cancer Biology 2022Quote: ... and CD8 alpha antigen retrieval was performed with BOND Epitope Retrieval 2 – ER2 (AR9640, Leica) for 40 ...
-
bioRxiv - Neuroscience 2021Quote: ... 2 μm z-steps) were taken using a laser scanning confocal microscope (TCS SP8, Leica). Three sections of 20 μm depth each were averaged per animal for analysis ...
-
bioRxiv - Developmental Biology 2022Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
-
CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system (Leica).
-
bioRxiv - Cell Biology 2020Quote: ... L4 animals were fixed by high-pressure freezing with the EMPACT-2 system (Leica Microsystems). Freeze-substitution (FS ...
-
bioRxiv - Immunology 2021Quote: ... samples underwent heat induced antigen retrieval (HIER) in BOND Epitope Retrieval 2 (AR9640, Leica Biosystems) or BOND Epitope Retrieval 1 (AR9961 ...
-
bioRxiv - Plant Biology 2023Quote: ... and images were taken every 20 min for 2 days using microscope (LEICA M205 FA). Imaging data were analyzed with ImagJ (FIJI).
-
bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... followed by an initial antigen retrieval using Epitope retrieval solution 2 (Leica Bond ER2, #AR9640) at 95°C for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: Freeze substitution was performed in a Leica EM AFS-2 freeze substitution unit (Leica Microsystems) as described previously (Bragulat-Teixidor et al. ...
-
bioRxiv - Immunology 2024Quote: ... Heating mediated-epitope/antigen retrieval was performed with Bond TM Epitope Retrieval 2 (Leica Biosystems). Blocking was performed with plant-based protein blocking buffer (Cell Signaling Technology [CST] ...
-
bioRxiv - Bioengineering 2022Quote: ... and the appropriate visualization system was applied – DAKO DAB (Cat# K346811-2, Leica, Heidelberg, Germany) for all but CD206 which utilized AEC (Cat# K400511-2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... the stained larvae were mounted in 2 % methylcellulose and photographed by a fluorescent stereomicroscope (Leica) or mounted in low-melting agarose gel and photographed by a confocal microscope (Olympus FV1000).
-
bioRxiv - Biochemistry 2024Quote: ... Grids were blotted for 2 s with an EM GP2 Plunge Freezing device (Leica Microsystems) held at 4 °C and 90% humidity before freezing in liquid ethane ...
-
bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
-
bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
-
bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
-
bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
-
Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
-
bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...