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Citations for Leica :
351 - 400 of 809 citations for 3 1 Phenylethylamino propane 1 thiol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... slides were baked at 37° C for 1 h prior to loading on a BOND RXm (Leica) for further baking ...
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bioRxiv - Genomics 2024Quote: ... and fibrillin 1 (FBN1) was performed using the Leica Bond RXM automated immunohistochemistry staining platform (Leica Biosystems). Before staining ...
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bioRxiv - Immunology 2024Quote: ... cells were counterstained with DAPI (1:1,000; Beyotime) and examined under Leica SP8 Confocal Microscope (Leica, Germany) with a 40× objective.
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) (Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Neuroscience 2024Quote: ... and the second against the rod domain (1:150) detecting longer dystrophin isoforms (NCL-DYSA, Leica Biosystems). The primary antibodies incubated for 60 minutes involved heating at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Antigen retrieval was achieved by immersing the sections with BOND Epitope Retrieval Solution 1 (Leica Bond, AR9961) for 20 minutes at 95°C ...
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bioRxiv - Bioengineering 2024Quote: ... Slides were allowed to dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera) ...
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bioRxiv - Immunology 2024Quote: ... JC-1 staining was visualized using a Leica SP8 laser scanning confocal microscope (Leica Microsystems, Heidelberg, Germany) with a 63 × oil objective ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were incubated in 3% hydrogen peroxide (Bond Polymer Refine Detection Kit DS9800, Leica Biosystems) for 15 mins to block endogenous peroxidases ...
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bioRxiv - Evolutionary Biology 2021Quote: ... embedded in 3% LM-agarose (Roth) and cut 250 µm-thick with a vibratome (Leica VT1000S). Slices were then transferred on Millicell inserts (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: Broad-band autofluorescence was acquired from sections cut at 3 μm using X20 objective (Leica DM550B). Sections were excited at 458 nm and fluorescence emission captured above 475 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... and glypican 3 (Cell Marque, #261M) was detected using BOND Polymer Refine Detection (Leica Biosystems, #DS9800), including a hematoxylin counterstain to visualize nuclei.
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bioRxiv - Developmental Biology 2020Quote: ... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
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bioRxiv - Cell Biology 2022Quote: ... and 3% water in acetone) and placed in an automated freeze substitution machine (AFS2, Leica Microsystems). A freeze substitution protocol was used as previously described16 ...
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bioRxiv - Neuroscience 2023Quote: High-pressure freezing: Reprogrammed cortical neurons were seeded and grown on 3 mm sapphire discs (Leica) until PID30 ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated with primary antibodies (Table 3) in Bond primary antibody diluent (AR9352, Leica) followed by secondary antibodies (Leica ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... followed by thin (∼3 nm) copper sputtering (Bal-tec MED 020, Leica Microsystemes SAS, Nanterre, France). Imaging was carried out using a Hitachi S800 FEG ...
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bioRxiv - Developmental Biology 2023Quote: Fixed brains were embedded in 3% noble agar gel and sectioned with the Vibratome VT1000S (Leica) at 50 μm thickness as free-floating sections ...
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bioRxiv - Neuroscience 2021Quote: Serial cryostat coronal brain sections at the level of Bregma 1 mm to 0.5 mm were prepared (Leica) for single cell isolation) ...
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bioRxiv - Bioengineering 2020Quote: ... Heat-induced antigen retrieval was performed on the Bond-Max using Epitope Retrieval 1 solution (Leica Biosystems Inc.) for 20 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µg ml−1 WGA-AF488) and observed with a TCS-SP8 confocal laser-scanning microscope (Leica Microsystems) under the following conditions ...
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bioRxiv - Pathology 2021Quote: ... the PFA-fixed brains were cut into 1 mm thick slices using a vibratome (Leica Biosystems, Germany, VT1200S). After bleaching with 5 % H2O2/PBS overnight at 4°C the samples were pre-permeabilized (0.2 % Triton X-100/PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were first warmed to 60⁰C for a 1 hour then dewaxed in Bond dewax (Leica, AR9222) for 30 mins before antigen retrieval in ER2(Leica ...
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bioRxiv - Neuroscience 2021Quote: ... Z-stack images of 1 µm interval (total depth of 4 µm) were processed with LAS software (Leica) and analyzed with Fiji software ...
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bioRxiv - Genetics 2020Quote: ... Whole-mount immunostaining serial sections of stage 1 ovules were captured on a laser scanning confocal microscope (Leica), with multitrack configuration for detecting PI (excitation with laser at 568 nm ...
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
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bioRxiv - Pathology 2022Quote: ... Sections were then incubated with the primary antibody (diluted at 1:1,600 in primary antibody diluent [Leica Biosystems]) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Transverse semi-thin (1 µm) sections of ON were cut on an ultramicrotome (EM UC7, Leica, Wetzlar, Germany) from tissue collected 2 mm distal to the eye and stained with 1% para-phenylenediamine (PPD ...
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bioRxiv - Neuroscience 2024Quote: ... Thirty µm coronal slices of hemispheres were cut at −18 ± 1°C using a cryostat (Leica #CM3050 S), and slices were directly mounted on Superfrost Plus™ slides (Fisher Scientific #1255015 ...
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bioRxiv - Cancer Biology 2024Quote: ... Each staining cycle concluded with an antigen retrieval step with Epitope retrieval solution 1 (Leica Bond ER1, #AR9961) at 95°C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... one semi-thin (1 μm) section was cut with a Leica EM UC6 ultramicrotome (Leica Microsystems, Vienna, Austria). Ultra-thin (60 nm thick ...
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bioRxiv - Cancer Biology 2023Quote: ... all samples were resuspended in PBS containing DAPI (1:3000) and imaged using a TCS SP5 II (Leica) confocal microscope.
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bioRxiv - Neuroscience 2024Quote: ... or secondary antibodies (Table 1) were acquired using the Fast Lifetime Contrast (FALCON) enabled tau-STED module (Leica) with the time-gate on HyD-X detectors adjusted between 0.1 and 6 nanoseconds.
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bioRxiv - Cancer Biology 2024Quote: ... Heat induced epitope retrieval was performed at 95°C using Bond Epitope Retrieval Solution 1 (Leica Biosystems AR9961) for transferrin receptor 1 (TfR1 ...
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bioRxiv - Pathology 2024Quote: ... using a TIS Camera USB 3.0 Monochrome 1/1.2 CMOS and on an inverted confocal microscope (Leica DMi8) with Stellaris 5 scan head ...
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bioRxiv - Microbiology 2024Quote: ... washed twice for 1 min with milli-Q water and visualized with an epifluorescence microscope (Leica, type DM4000B).
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bioRxiv - Cell Biology 2024Quote: ... AID-NUP358 DLD-1 were transfected with the NLS-mCherry-LEXY (pDN122) for 24h and analyzed by Leica Sp5 confocal inverted microscope (DMI6000 ...
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bioRxiv - Cell Biology 2020Quote: ... Images were prepared with the Leica Application Suite – Advanced Fluorescence (LAS-AF) Version 3 software (Leica Microsystems).
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bioRxiv - Neuroscience 2020Quote: ... the Ca2+ signal within the SNs was captured during 3-min using Leica DM6000 microscope (Leica Microsystems) and processed with the software Fiji image J ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL) to a thickness of 30 µm ...
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bioRxiv - Cell Biology 2020Quote: ... The surface area of cardiomyocytes stained by MHC was determined with image analysis software (Leica Qwin 3) and calculated as the mean of 100 to 120 cells from randomly selected fields.
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bioRxiv - Cell Biology 2021Quote: ... were recorded and the maximal projection was deconvoluted with 3 iterations by the LAS AF 2.7.3.9 software (Leica).
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bioRxiv - Biochemistry 2021Quote: ... for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems). Datasets were collected at the NIH Multi-Institute Cryo-EM Facility (MICEF ...
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bioRxiv - Physiology 2020Quote: ... mexicanum gametes was imaged using FluoZin-3 and a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Leica 506224 5X objective ...
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bioRxiv - Neuroscience 2020Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
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bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
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bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...