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Citations for Leica :
351 - 400 of 2149 citations for 1R 2S 6R 7S 4 4 DIMETHYL 3 5 DIOXA 8 AZATRICYCLO 5.2.1.0 2 6 DECAN 9 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin embedded sections were then sliced into 4 μm-thick slices on a Leica RM2016 Microtome (Leica, Shanghai, China) for immunohistochemistry staining and apoptotic cells labeling ...
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bioRxiv - Cell Biology 2022Quote: ... the pulp chambers of the mandibular first molars were opened by a high-speed handpiece with #1/4 carbide round bur under direct visualization by a stereoscopic microscope (Leica). A #10 endodontic K file was then used to probe the root canals ...
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bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 4% agarose before being cut into 40 um coronal slices with a vibratome (Leica VT S10000) and stored in 0.1% PBS Sodium Azide ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v) sucrose for two days and then embedded in tissue freezing medium (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... larvae were embedded in 4% agarose in PBS and 50-100 μm sections were obtained using a vibratome (Leica, VT1200S). Sections were counterstained with DAPI (Thermo Fisher Scientific Cat#62248 ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were kept in 4% PFA at least overnight and stored in PBS until histological slicing (Leica VT1200 S). DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
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bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2024Quote: ... electroporated cerebella were embedded in 4% LMP agarose dissolved in mHBSS and then cut into 300 mm sagittal slices using a VT1200 vibratome (Leica). The slices were finally placed on 0.4 mm PTFE cell culture inserts (Millicell ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were stored at 4°C and imaged using the Inverted Leica DMIL LED Microscope with the Thunder imaging software (Leica).
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bioRxiv - Genomics 2024Quote: ... Sections of 4 μm without wax were stained with safranin and fast green and photographed with electric inverted fluorescence microscope (MDi8, Leica).
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bioRxiv - Bioengineering 2023Quote: ... The brains were extracted and postfixed for 24-48 hours in the same fixative at 4°C before being sliced into 50 μm coronal sections using a vibratome (Leica). The slices were blocked in 10% normal goat serum (SouthernBiotech ...
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bioRxiv - Neuroscience 2023Quote: ... Cell aggregates and transplanted retinas harvested from RD-nude rats were fixed with 4% paraformaldehyde (Wako) and sectioned with a cryostat (Leica) to prepare frozen sections ...
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bioRxiv - Neuroscience 2023Quote: ... for 50-μm sections, the brains were embedded in 4% agar (01059-85, Nacalai Tesque) and sliced using a vibratome (VT1200S, Leica). For immunohistochemistry ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then coated with a 4 nm layer of platinum using a ACE600 high resolution sputter coater (Leica Microsystems) before being imaged using an FEI Quanta 450 ESEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Molecular Biology 2023Quote: ... Immunohistochemistry or immunofluorescence experiments were performed on 4 µm-sections using Novolink polymer detection system (7140-K, Leica Micro-systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Processed tissues were embedded in paraffin and sectioned at 4 microns on a rotary microtome (Leica Biosystems, Buffalo Grove, IL). Tissues were mounted on glass slides and stained with hematoxylin and eosin using routine protocols on an automated histostainer (Leica ST5010 Autostainer ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... The harvested tissues were then subsequently fixed with 4% paraformaldehyde (v/v) at 4°C and sliced into 40-μm sagittal and axial sections using a CM3050 S cryostat (Leica).
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bioRxiv - Developmental Biology 2023Quote: ... Undecalcified frozen sections at a thickness of 4 µm were obtained according to Kawamoto’s film method using TC-65 (Leica Microsystems) or SL-T35 (UF ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Bioengineering 2024Quote: ... and finally coverslips were mounted on SEM stubs with carbon tape and metallized with 4 nm of platinum (Leica ACE600). Electron microscopy was carried out using a field emission gun scanning electron microscope (SEM ...
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bioRxiv - Bioengineering 2024Quote: ... at 4°C and embedded in OCT for generation of 15 µm coronal sections using a CM3050 S cryostat (Leica).
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bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA overnight and then sliced into 100 um coronal sections using a vibratome (VT1200s, Leica). Brain slices were imaged by a fluorescence microscope (BX63 ...
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bioRxiv - Neuroscience 2024Quote: ... containing 4 μM QSY21 to quench the extracellular QD signal by using a confocal laser scanning microscope (TCS SP8, Leica) at a 1 Hz rate ...
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bioRxiv - Neuroscience 2024Quote: Perfused brains (4% paraformaldehyde) were sectioned at 50-60 µm using a vibratome (Leica VT1000, Leica Microsystems, Nussloch GmbH, Germany). Sections were washed twice for 5 minutes each in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: Mice brains were harvested after perfusion with 4% PFA in PBS1X and post-fixed in 4% PFA for 2 hours on ice or at 4°C.Brains were then washed with 1XPBS three times and cut in cold PBS1X using a vibratome (Leica VT1200S), in 40 µm thick sections.Brain sections were mounted onto positively charged microscope slides (Superfrost PlusTM Microscope Slides White Tab ...
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bioRxiv - Cell Biology 2024Quote: Mice lung specimens were fixed in 4% paraformaldehyde for 48 h and subsequently embedded in paraffin using ASP200S and EG1150H (Leica). At the time of death ...
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bioRxiv - Cell Biology 2024Quote: ... kidneys were embedded in low-melting agarose (4%) and cut into 300 µm slices using a vibratome (VT1200 S, Leica). Slices were maintained in Krebs-Henseleit-Buffer carbonated with 95% O2/5% CO2.[24] For imaging ...
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bioRxiv - Plant Biology 2021Quote: ... the embedded samples were sectioned at 2–5 μm thickness using a rotary microtome (Leica, Wetzlar, Germany) and stained with basic fuchsin ...
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bioRxiv - Developmental Biology 2022Quote: ... one by one taken out of the medium and positioned along the anteroposterior axis under a stereo microscope (Leica MZ16 FA) in a 1.5% agarose gel (Sigma Aldrich IX-A ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on an FE-SEM (Zeiss Merlin ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Molecular Biology 2022Quote: ... The glycolytic and oxidative portions of the TA muscles of 5 mice in the CTR group and 5 mice in the Crush - 3 days group were microdissected with a laser microdissection system (Leica LMD6) and recovered in RNAlater reagent (QIAGEN).
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bioRxiv - Neuroscience 2020Quote: ... Spinal cords were removed from the spinal column by hydraulic extrusion and embedded in low-melting point agarose (2-3%) before sectioning using a vibratome (Leica) into 300 µm thick coronal slices in ice-cold oxygenated ACSF ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035 ...
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC ...
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bioRxiv - Microbiology 2024Quote: ... the eYFP fluorescence of fusion proteins was analyzed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8).
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bioRxiv - Developmental Biology 2021Quote: ... when embryos were fixed by 4% PFA for ensuing WISH analysis or imaged using a Fluorescent stereoscopic microscope (LEICA M205 FCA). 10 pg of gsc mRNA and 10 pg of ndr2 mRNA were injected ...
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bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...