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Citations for Leica :
351 - 400 of 1328 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... The resin blocks were cut longitudinally into 6-μm slices of the tibia using a Leica RM2165 rotary microtome equipped with a tungsten blade (Leica Microsystems, Germany). Fluorescence signals emitted by calcein green and alizarin red were recorded using a fluorescence microscope (Leica Microsystems) ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Perfused brains (n = 20 / sex; 40 total) were sliced coronally into 6 series at 30 µm on a vibrating microtome (Leica VT 1000S). The samples were stored at -20 °C in cryoprotectant until immunohistochemical (IHC ...
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bioRxiv - Cell Biology 2020Quote: ... Images were prepared with the Leica Application Suite – Advanced Fluorescence (LAS-AF) Version 3 software (Leica Microsystems).
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bioRxiv - Neuroscience 2020Quote: ... the Ca2+ signal within the SNs was captured during 3-min using Leica DM6000 microscope (Leica Microsystems) and processed with the software Fiji image J ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL) to a thickness of 30 µm ...
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bioRxiv - Cell Biology 2020Quote: ... The surface area of cardiomyocytes stained by MHC was determined with image analysis software (Leica Qwin 3) and calculated as the mean of 100 to 120 cells from randomly selected fields.
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bioRxiv - Cell Biology 2021Quote: ... were recorded and the maximal projection was deconvoluted with 3 iterations by the LAS AF 2.7.3.9 software (Leica).
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bioRxiv - Biochemistry 2021Quote: ... for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems). Datasets were collected at the NIH Multi-Institute Cryo-EM Facility (MICEF ...
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bioRxiv - Physiology 2020Quote: ... mexicanum gametes was imaged using FluoZin-3 and a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Leica 506224 5X objective ...
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bioRxiv - Neuroscience 2020Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
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bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
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bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...
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bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...
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bioRxiv - Cell Biology 2022Quote: ... and images were acquired using a 3-channel TCS SP2 laser-scanning confocal microscope (Leica Mycrosystems, Germany) and STELLARIS 8 ...
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bioRxiv - Molecular Biology 2023Quote: 2.8 × 105 A549 or A549-IFITM3 cells were seeded on carbon- coated 3 mm sapphire discs (Leica) in a 6-well plate in complete growth medium (DMEM-F12 ...
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bioRxiv - Immunology 2024Quote: ... Nine TLS+ Glioma FFPE sections (3 µm) were generated using the Leica SM 2000R microtome (Leica Microsystems), and incubated at 37 C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... synovial tissue sections at 3 µm thickness were prepared using a HistoCore Autocut microtome (Leica Biosystems, Switzerland) from FFPE blocks and transferred onto Superfrost® Plus Menzel (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and the contusion was induced using a 3 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 0.95 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Cell Biology 2020Quote: ... Brain images were acquired in a 4% PFA solution using a stereomicroscope (M80, Leica Microsystems).
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bioRxiv - Bioengineering 2021Quote: ... DLP-printed moulds were sputter-coated with 4 nm platinum (Leica EM ACE600, Wetzlar, Germany) And imaged with a Phenom XL G2 Desktop SEM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... They were kept at 4 °C until cutting at the cryostat (CM3050S; Leica Wetzlar, Germany). We produced 20-μm thick coronal sections of the primary motor cortex (M1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Mounted slides were stored at 4°C until imaged using LAS AF software (Leica Microsystems) on a Leica TCS SP8 confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: Brains perfused in 4% PFA were sectioned into 80 µm slices using a Vibratome (Leica), then mounted and stained with cresyl violet to reconstruct lesion size ...
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bioRxiv - Immunology 2023Quote: ... 4 μm-thick segments of the fragments were prepared in a semiautomatic microtome (Leica®) and organized on microscopic slides ...
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bioRxiv - Biophysics 2023Quote: ... Ultrathin sections (60 -80 nm) were obtained using Leica UCT 4 ultramicrotome (Leica, Wetzlar, Germany) and stained with uranyl acetate ...
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bioRxiv - Cell Biology 2023Quote: ... The 4 µm plastic sections were cut with a rotational microtome (Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2024Quote: ... overnight at 4°C and were subsequently embedded in tissue freezing medium (Leica, Richmond, USA). Frozen embedded tissues were cut in 14 μm sections and mounted on SuperFrost Plus Microscope slides (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 4 to 5-week-old colonies were imaged using the stereo microscope (M205 FA, Leica), and the area was determined by applying thresholding to convert reflected light 8-bit images to 1-bit images and analyzed using Fiji software (ImageJ 1.54f ...
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bioRxiv - Developmental Biology 2024Quote: 4-cell stage classification: Live imaging was performed with a SP5 scanning confocal microscope (Leica) using the 63x oil objective ...
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bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... resin blocks were mounted onto SEM pins with silver epoxy and sputter coated with 6 nm of iridium (Leica ACE 600, Vienna, Austria) at a 45° angle with rotation on a planetary stage to ensure the entire block was coated.
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bioRxiv - Neuroscience 2023Quote: ... Brain regions containing the possible contact sites were re-embedded into resin blocks and 70 nm thick sections were cut with an ultra-microtome (Leica EM UC 6). Electron microscopic images were taken either with a Hitachi H-7100 transmission electron microscope or a FEI series electron microscope to reveal synaptic contacts between BFPVNs and target cell types in the MS ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were sectioned at 50 µm or 6 μm on a Leica CM3050 S cryostat set to −20°C for both the object and chamber temperature (Leica Biosystems, Nussloch, Germany). We collected sections from the primary somatosensory cortex (coronal sections from bregma ...
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bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
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bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...
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bioRxiv - Microbiology 2021Quote: ... Confocal imaging was performed with a Leica TCS SP8 STED 3× laser scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 100×/1.4 Oil HC PL APO CS2 STED White objective ...
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bioRxiv - Neuroscience 2022Quote: ... then following fixation they were imaged with an inverted confocal microscope with the laser power increasing for 0.2 to 3% with 0.2% intervals (Extended Data Fig. 1a, b, SP8 TCS with HyD detectors, Leica). Laser power was linearly controlled by ATOF ...
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bioRxiv - Plant Biology 2022Quote: ... leaves were harvested at 3 dpi for aniline blue/calcofluor staining and analysis by epi-fluorescence microscopy (Leica DM6B ...
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bioRxiv - Plant Biology 2022Quote: ... GUS-stained leaves were embedded in 3% agarose and sectioned with a vibrating blade microtome (Leica VT1000 S). Images were taken using ZEISS Axio Observer.
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bioRxiv - Neuroscience 2023Quote: ... the ISH-mounted specimen was first loaded into a pre-tilted specimen block (Leica; Figure 3 J&K). The block was then placed into a Leica VCT-100 cryo-transfer system under liquid nitrogen to facilitate transfer into the pre-cooled cryo-FIB/SEM cryo-substage.
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bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
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bioRxiv - Cell Biology 2023Quote: ... The cryosection ribbon (∼3 mm long) was then attached to the grid by operating the Crion (Leica Microsystems) in “charge” mode for ∼30 seconds (Pierson et al. ...