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Citations for Leica :
301 - 350 of 450 citations for Mouse Anti Clostridium Difficile Toxin A Antibody TA5 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: 5 µm sections of formalin-fixed and paraffin-embedded (FFPE) mouse tail specimens were H&E-stained by using an automated stainer (Leica XL) at University of Manchester’s Histology Core as previously described [11 ...
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bioRxiv - Biochemistry 2023Quote: ... Rabbit mAb (Mouse Preferred; IHC Formulated) (CST, #12202)) was used 1:200 and developed with Bond Polymer Refine Detection (Leica, DS9800).
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bioRxiv - Immunology 2024Quote: ... Glomerular area was quantified and presented as the mean area of 12-15 glomeruli per mouse using the Aperio ImageScope software (Leica, V12.4.6.5003)25.
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bioRxiv - Molecular Biology 2020Quote: ... Antibody signals were detected on a Leica DM6000 microscope equipped with a DFC390 camera (Leica). Data acquisition was performed with LAS AF (Leica) ...
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bioRxiv - Cancer Biology 2020Quote: ... then the samples were incubated in polymeric horseradish peroxidase (HRP) conjugated secondary antibody (Leica PV6121) for one hour followed by 5 five-minute 1xTBST washes ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were diluted as follows: p53 (CM5) (1:500, NCL-L-p53-CM5p, Leica Biosystems), p21 (F-5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL ...
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bioRxiv - Cancer Biology 2024Quote: ... IgG (0.5 µg) provided with the kit or 1 µL p53 antibody (CM5, Leica Biosystems) were used for immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... The staining conditions for all primary antibodies were optimized using chromogenic DAB detection (Leica Biosystems). Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated with the corresponding secondary antibodies and visualization systems (Novolink Polymer Linker, Leica) conjugated with horseradish peroxidase ...
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bioRxiv - Developmental Biology 2023Quote: ... The antibody information was listed in Supplementary Table 13 and images were taken from Leica TCS SP8 confocal laser-scanning microscope.
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bioRxiv - Cell Biology 2023Quote: ... The staining conditions for all primary antibodies were optimized using chromogenic DAB detection (Leica Biosystems). Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies used for staining included the following: HHV8/LANA (cat# PA0050, Leica, Bannockburn, IL), CD31 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by anti-rabbit conjugated to horseradish peroxidase (Powervision, Leica)
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bioRxiv - Cancer Biology 2020Quote: ... anti-calretinin (Leica Biosystems: NCL-L-CALRET-566, 1:200), anti-keratin 5 (Biolegend ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-Ki67 (1:100, Leica Biosystems no. NCL-Ki67p), rabbit anti-Slc1a3(1:300 ...
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bioRxiv - Cancer Biology 2022Quote: ... 6) Opal 780/anti-Ki67 (Ready-to-use, MM1, Leica). Slides were cover-slipped using ProLong™ Diamond Antifade Mountant (Invitrogen) ...
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bioRxiv - Pathology 2023Quote: ... Slides were subsequently incubated with anti-CK5 (1:200, Leica Biosystems ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Dystrophin (Novocastra/Leica Biosystems, clone DY4/6D3, 1:200), anti-Laminin (SigmaAldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... with the following Abs: Anti-human Abs against CD19 (Leica #NCL-L-CD19-163 ...
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bioRxiv - Immunology 2023Quote: ... and subsequently incubated with an anti-rabbit HRP polymer (Leica) and colorized with 3,3’-Diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-CD20 clone L26 (CD20-L26-L-CE, Leica Biosystems) for 15 min at a concentration of 0.19 µg/mL using Antibody Diluent ...
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bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections of the mouse brain containing the dentate gyrus and prefrontal cortex were then cut using a VT1200S microslicer (300 μm) (Leica, Wetzlar, Germany). Micro-dissected areas were post-fixed at 4C for 2 hours in 2% osmium tetroxide in 0.1mol/l Cacodylate Buffer ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) and placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany). Mice were given a subcutaneous injection of meloxicam (2 mg/kg ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Physiology 2024Quote: ... an area of each section (1 section/mouse) was acquired at 200x magnification using a confocal laser scanning microscope (Leica TCS SP8). Sequential scanning was employed using the 488 nm laser to visualize Alexa 488 and the 405 nm UV lasers to visualize DAPI staining ...
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bioRxiv - Neuroscience 2024Quote: In situ hybridization was performed on 16 μm thick midbrain slices from a fresh-frozen mouse brain cut on a cryostat (Leica CM3050 S). All FISH reagents used are commercially available from ACD bio ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody staining was completed using the Bond Polymer Refine IHC protocol and reagents (Leica, Wetzlar, Germany). Slides were counterstained on the Leica Autostainer XL (Leica ...
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bioRxiv - Genetics 2021Quote: ... Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were used ...
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bioRxiv - Cancer Biology 2020Quote: ... The TMA slides were sequentially stained with antibodies specific for CD4 (T cells, 4B12, Leica Biosystems), CD8 (T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were applied for 60 mins and detected with Polymer Refine Detection Kit (Leica, DS9800). For detection of P63 ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical analyses were performed utilizing ready-to-use Ki67-specific antibody solution (K2, Leica Biosystems, PA0230). Sections were dewaxed ...
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bioRxiv - Immunology 2024Quote: ... Ready to Use Antibody reagent was added as marker and incubated for 15 min (CD4: Leica Bio systems ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-Trp53 (1:500; Novocastra Leica, NCL-L-p53-CM5P), goat anti-Pdgfra (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-α-SMA (NCL-L-SMA, Leica Biosystems, dilution: 1:500), anti-COL1 (1310-01 ...
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
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bioRxiv - Cell Biology 2024Quote: ... Fresh-frozen wildtype mouse kidneys were embedded in OCT (Sakura) and sectioned at 10 µm using a rotational cryomicrotome (HM500 Leica Microsystems, Wetzlar, Germany) and collected on super frost slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse plantaris muscles preserved for IHC were sectioned at a thickness of 7 μm using a cryotome (Leica Biosystems; Buffalo Grove, IL, USA) and adhered to multiple positively charged slides for the interrogation of various targets as explained in this and subsequent paragraphs ...
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bioRxiv - Bioengineering 2024Quote: ... Morphology of the collagen fibers in aged and young mouse breast tissues and tail tendons were visualized through second harmonic imaging (SHG) with a two-photon microscope (Leica Stellaris 8 Dive). SHG imaging was performed at 25x magnification with optical zoom on the specific sites.
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bioRxiv - Microbiology 2021Quote: ... For both antibodies antigen unmasking was undertaken using Heat Induced Epitope Retrieval (HEIR) solution 1 (Leica AR9961) for 30 minutes at 100°C.
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...