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Citations for Leica :
301 - 350 of 656 citations for Anti Swiprosin 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibody (polymer) incubation (10 minutes) and diaminobenzidine-based signal generation (10 minutes) were performed per manufacturer’s instructions (Leica # DS9800). Pictures were taken using SPOT Insight Gigabit camera and SPOT Imaging Software (5.1).
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated for 60 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed and stained with appropriate secondary antibodies and imaged using an inverted laser scanning confocal microscope (TCS SP5 X, Leica) using Leica Application Software (LAS ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were incubated for 30 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... IHC staining for Ki67 and MGP antibodies was performed using Bond III IHC autostainer for full Automated Immunohistochemistry (Leica biosystems). Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes: CD20 (Leica Biosystems PA0200), 1:1 with CF488 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD4 (eBioscience 14-9766-82, ER2 Leica, 1:500), CD8 (eBioscience 14-0808-82 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8 (eBioscience 14-0808-82, ER2 Leica, 1:500), Ly6G (clone 1A8 ...
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bioRxiv - Cancer Biology 2020Quote: ... CK8/18 (Novocastra/Leica; 1/50; NCL-L-5D3), p63 (4A4 ...
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bioRxiv - Cell Biology 2022Quote: ... or EM GP Model 1 plunge freezer (Leica Microsystems). For all samples ...
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bioRxiv - Biochemistry 2023Quote: ... 1 minute in Hematoxylin (Leica SelecTech 560, Richmond, IL), washed in dH2O x3 on/off ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8a (eBioscience 14-0808-82, 1:500, ER2 Leica), CD4 (eBioscience 14-9766-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD4 (eBioscience 14-9766-82, 1:500, ER2 Leica), FoxP3 (CST 12653 ...
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bioRxiv - Bioengineering 2020Quote: ... Slides were incubated with an anti-rabbit-conjugated horseradish peroxidase (HRP; Leica, Wetzlar, Germany) for 8 minutes and colorized with 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies were washed in TBS three times for three minutes at a time before the Novolink post primary block (Leica biosystems) was applied for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Immunology 2021Quote: Primary (1ry) Antibodies were incubated for one hour and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
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bioRxiv - Pathology 2022Quote: ... slides were stained and processed using the 17B4 primary antibody on a Leica BOND-III autostainer (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Immunology 2019Quote: ... antibodies were used for staining with Bond Refine polymer staining kit on the Bond Max automated staining system (Leica Biosystems, Germany). All stained slides were dehydrated in ascending ethanol and xylene washes before coverslipping with cytoseal (Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL, Leica microsystems, GmbH, Wetzlar, Germany) at 20 × magnification ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Genomics 2022Quote: Five μm sections of formalin-fixed paraffin-embedded tissues from postmenopausal women were stained with hematoxylin and eosin or with commercially available antibodies using the Leica Bond RX automated stainer (Leica Biosystems) and reviewed by gynecologic pathologists ...
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bioRxiv - Cell Biology 2023Quote: ... and immunostained using primary and secondary antibodies (listed in ESM Methods) and imaged using a TCS SP5 confocal microscope (Leica, Germany). Immunostaining of paraffin-embedded pancreas from mice with beta cell-selective deletion of Dnmt3a was performed according to [10 ...
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bioRxiv - Microbiology 2019Quote: ... each for 30 min at room temperature and 100% for 2 × 1 h and 1 × 30 min) and dried in a Leica EM CPD030 (Leica Microsystems Belgium, Diegem, Belgium). The membranes were mounted on a stub and coated with 5 nm of carbon (Leica Microsystems Belgium ...
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Immunology 2022Quote: ... Detection was performed using PowerVision Poly-HRP Anti-Rabbit IgG Novocastra (Leica Biosystems, Cat. PV6119) for 30-minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred to TBST for 5 min and incubated with anti-rabbit poly-HRP-IgG (Leica Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... and completely covered with post-primary rabbit anti-mouse IgG (Leica Biosystems, Novolink, # RE7260-K) for 30 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with PowerVision polymeric horseradish peroxidase (poly-HRP) anti-mouse IgG (Leica Biosystems) or PowerVision polyHRP anti-rabbit IgG for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... and visualization systems (Omni Map anti-Rabbit, Ventana, Roche; Bond Polymer Refine Detection, Bond, Leica) conjugated with horseradish peroxidase ...
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bioRxiv - Molecular Biology 2023Quote: ... and visualization systems (Bond Polymer Refine Detection, Bond, Leica; Omni Map anti-(rabbit,mouse,rat) Ventana ...
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bioRxiv - Developmental Biology 2020Quote: ... α-sarcoglycane (1/150, A-SARC-L-CE, Leica biosystems), γ-sarcoglycane (1/150 ...
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bioRxiv - Cell Biology 2020Quote: ... and CD56 (Leica Biosystems, NCL-L-CD56-504; 1:100). Unbound primary antibody was removed by washing and cells stained for 1 hr at RT with Alexa Fluor-488 anti-rat secondary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... setup #1 was operating a fast emission filter wheel (Leica) equipped with the custom bandpass filters ET455/50 ...
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bioRxiv - Immunology 2023Quote: ... setup #1 was coupled to a mercury lamp (Leica EL6000) and setup #2 was coupled to a xenon lamp (Lambda LS lamp ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1 mm sections were prepared using vibratome (Leica; VT1000S). 1 mm sections that contain the aortic and mitral valves were collected and trimmed into 1 mm cubes to remove other tissues ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... slides were pretreated with antigen retrieval solution 1 (ER1, Leica) for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica) ...
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bioRxiv - Molecular Biology 2023Quote: ... Heat-based retrieval with Bond Epitope Retrieval 1 (Leica Biosystems) was finally performed for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... and confocal (1 μm optical sections, Leica TCS SP8 confocal) images were captured at the same settings and at the same time for all sections within an experimental group.
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bioRxiv - Cancer Biology 2023Quote: ... and for antigen retrieval using Epitope Retrieval 1 (Leica™) solution at 100°C for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... was applied on tissue sections for 25 minutes incubation and the antigen-antibody binding was detected with Bond polymer refine detection (Leica Biosystems, DS9800). The tissue sections were covered with cover glasses ...
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bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4 (dilution 1:80; Leica Biosystems, CD4-368-L-CE-H) and CD8 (dilution 1:25 ...
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bioRxiv - Immunology 2023Quote: ... All setups were enclosed with a heating chamber (Leica (setup #1), Pecon (setup #2) ...
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bioRxiv - Immunology 2023Quote: ... Setup #1 was additionally operated with the Leica software (Leica Suite) and µManager (Open Imaging) ...
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bioRxiv - Cell Biology 2023Quote: ... were assembled on 1-well cups for SampLink chambers (Leica Microsystems). The assembled SamplLink chambers were washed in 70% ethanol ...
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bioRxiv - Immunology 2023Quote: ... Samples were treated with BOND Epitope Retrieval Solution 1 (Leica AR9961) for CD4 and CD68 antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared at a thickness of 70 μm for imaging intrinsic fluorescence or 40 µm for antibody staining (Leica VT 1000S vibratome). For immunohistochemistry ...
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bioRxiv - Neuroscience 2023Quote: ... Colocalization of the antibody immunofluorescence signal and endogenous fluorescence of NG2 glia was examined using the Leica Stellaris confocal platform (Leica Microsystems, Wetzlar, Germany). The immunopositive areas of the cortex surrounding the ischemic injury were further quantified using Fiji ImageJ software (NIH ...