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Citations for Leica :
301 - 350 of 512 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated in primary antibody solution made with 1:250 mouse anti-Ki67 (Leica Biosystems), 1% NDS ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Neuroscience 2024Quote: ... Brains stored in 30% sucrose cyroprotectant solution were sectioned on a freezing microtome (Leica Biosystems, SM2010 R) before being transferred to PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were then sectioned in the same ice-cold sucrose cutting solution using a vibratome (VT-1200s, Leica). For sections from motor cortex ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) (Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Antigen retrieval was achieved by immersing the sections with BOND Epitope Retrieval Solution 1 (Leica Bond, AR9961) for 20 minutes at 95°C ...
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bioRxiv - Neuroscience 2021Quote: ... 40pm horizontal sections of the thalamus (D/V depth −5.3 mm to 6.0 mm) were obtained using a Leica VT 1000S microtome (Leica Biostystems, Buffalo Grove, IL, USA) and collected in 0.1 M phosphate buffer (PB ...
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bioRxiv - Plant Biology 2021Quote: ... The root segments were embedded in agarose 5% (w/v) and cut into 25-30 μm radial sections with a VT1200S vibratome (Leica Microsystems, Nanterre, France). Radial sections were sampled from 15 ectomycorrhizal rootlets (at 200 µm from the root apex ...
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bioRxiv - Neuroscience 2022Quote: Centimeter-thick tissue blocks of the desired brain regions were excised and cut on a vibratome (Microm HM 650 V, Thermo Scientific/Microm, Walldorf, Germany and VT1200S vibratome, Leica, Wetzlar, Germany) into 100 µm thick sections ...
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bioRxiv - Plant Biology 2022Quote: ... Inflorescence stems subjected to GUS staining were then embedded in 8% (w/v) agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Plant Biology 2023Quote: ... The fixed samples were embedded in 6% (w/v) low melting temperature agarose (Biozym) and longitudinal sections (70 μm thickness) were obtained using a VT1000S vibratome microtome (Leica Biosystems, Mannheim, Germany).
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bioRxiv - Bioengineering 2021Quote: ... or Toluidine Blue Stain, 1% w/v (Ricca Chemical, Arlington, TX) and scanned with a Leica Aperio ScanScope® CS2 (Leica, Buffalo Brove, IL) system ...
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bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Plant Biology 2023Quote: The quantification of the microscopy localization data of EIN2-GFP in Figure 7B was done according to (Fu et al., 2021) using LAS X (Leica Application Suite v. X3.1.1.15751) and GraphPad Prism V9.5.1 ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were then transferred to a 30 % sucrose solution and sliced coronally using a cryostat (Leica CM1850). Slices were mounted with DAPI-mounting medium (Vector Laboratories ...
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bioRxiv - Genomics 2020Quote: ... in 4μl of Ringer’s solution (made with DEPC water) + 0.01% Tween 20 and dissected under a stereomicroscope (Leica M80) with an X-ACTO knife (Amazon cat# B01MUWTH89) ...
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bioRxiv - Bioengineering 2020Quote: ... Heat-induced antigen retrieval was performed on the Bond-Max using Epitope Retrieval 1 solution (Leica Biosystems Inc.) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... mounted in Clearsee Alpha solution and observed using a confocal TCS SP5 or TCS SP8 confocal microscope (Leica). Auramine O was excited at 488 nm and the emission was collected at 500-570 nm.
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bioRxiv - Neuroscience 2020Quote: ... 300 μm-thick transverse slices were prepared in the cutting solution using a vibratome (Leica VT1200, Nussloch, Germany), and incubated at 34 °C for 30 min in the same solution ...
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bioRxiv - Cell Biology 2022Quote: ... The staining solution was removed and the samples were then imaged under a fluorescence microscope (DMi8, Leica, Germany) with 494 nm (green ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions: After antigen retrieval in a pH9 EDTA buffer based epitope retrieval solution (Leica), sections were incubated with monoclonal antibodies against Ki67 (rabbit anti-Ki67 ...
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bioRxiv - Bioengineering 2022Quote: ... Cell pellets were reconstituted in rat serum and stained with Leishman solution on frosted glass slides (Leica Biosystems). Using a light microscope (BX2 ...
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bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... Each staining cycle concluded with an antigen retrieval step with Epitope retrieval solution 1 (Leica Bond ER1, #AR9961) at 95°C for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were loaded into Leica Bond RX and pretreated with EDTA-based epitope retrieval ER2 solution (Leica, AR9640) for 20 mins at 95°C ...
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bioRxiv - Physiology 2023Quote: ... Coronal spinal cord slices (400 µm) were cut in the cutting solution using a vibratome (VT-1200S, Leica) and transferred to artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Molecular Biology 2023Quote: ... All solutions and high-pressure frozen sapphire discs were loaded to the automated freeze substitution system AFS2 (Leica). A freeze substitution program was performed according to the following table:
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bioRxiv - Cancer Biology 2024Quote: ... Heat induced epitope retrieval was performed at 95°C using Bond Epitope Retrieval Solution 1 (Leica Biosystems AR9961) for transferrin receptor 1 (TfR1 ...
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bioRxiv - Microbiology 2024Quote: ... and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems, Wetzlar, Germany). All fixed tissues were then processed in ethanol and xylene in a tissue processor and embedded in paraffin (Leica ASP6025 ...
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bioRxiv - Microbiology 2024Quote: ... with a tissue pretreatment of 30 minutes at 100°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) and 1:750 dilution of OPAL 570 (AKOYA ...
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Microbiology 2020Quote: ... heat-induced epitope retrieval (HIER) was performed using a ready-to-use citrate-based solution (pH 6.0; Leica Biosystems) at 100 °C for 20 min ...
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bioRxiv - Genomics 2020Quote: ... in 4μl of M9 (made with DEPC water) + 0.01% Tween 20 solution and dissected under a stereomicroscope (Leica M80) with an X-ACTO knife (Amazon cat# B01MUWTH89).
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bioRxiv - Neuroscience 2020Quote: ... Coronal sections of dLGN (320 μm) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were carefully extracted and transferred to a chamber filled with the same solution on a vibratome (VT1200; Leica). Brains were sliced at 250 μm (coronally for recording of SNr and PF neurons ...
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Cell Biology 2020Quote: 10 μL of the nuclei solution was pipetted on a hemocytometer (Neubauer) for visualization under the (Leica DM IL inverted) microscope ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections of VISp (320 μm) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21-25°C ...
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bioRxiv - Pathology 2022Quote: ... heat-induced epitope retrieval (HIER) was performed using a ready-to-use citrate-based solution (pH 6.0; Leica Biosystems) at 100°C for 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... Stem segments were embedded in 8% agarose solution and sectioned with a VT100 S vibratome (Leica, https://www.leica-microsystems.com/). Cross-sections were stained with a FASGA staining solution prepared as described in Tolivia and Tolivia (1987 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue blocks were cut coronally with a thickness of 300 μm in this solution using a vibratome (Leica VT1200S). Slices were collected and then incubated in aerated ACSF at 35 °C ...
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bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
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bioRxiv - Neuroscience 2022Quote: ... the apical turn cochlear organs from 1-2 months old Pclowt/wt and Pclogt/gt rats were dissected in ice-cold HEPES Hank’s solution and mounted onto type A specimen carriers (Leica Microsystems ...