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Citations for Leica :
301 - 350 of 944 citations for 6 Cyano imidazo 1 2 a pyridine 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Plant Biology 2020Quote: ... mesophyll cells of 2-week-old transgenic Arabidopsis plants expressing Raf36-GFP were observed with a confocal microscope SP8X (Leica Microsystems) with the time-gating method (Kodama ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal slices (300 µm thick) containing visual cortex were prepared using a vibrating blade microtome (Leica VT1200S or Campden 7000smz-2). Slices were immediately transferred to a submerged holding chamber with regular ACSF containing (in mM) ...
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bioRxiv - Plant Biology 2021Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Immunology 2021Quote: ... Ultra-thin (50 nm to 60 nm) sections were sectioned with a diamond knife (Jumdi, 2 mm) on an ultramicrotome (Leica Ultracut), contrasted with 2% uranyl acetate (Polyscience ...
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bioRxiv - Neuroscience 2022Quote: ... timelapse phase contrast images were acquired for 2 h at 37 °C with a 10X or 20X fluotar objective on a Leica DMIRE2 inverted microscope (Leica, Germany) equipped with a MS-2000 XYZ automated stage (ASI ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were first deparaffinized and then underwent antigen retrieval by incubating with EDTA-based epitope retrieval solution (BOND Epitope Retrieval Solution 2, Cas#AR9640, Leica BioSystems) for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Z-stacks of the cuticular side of the VL3 or VL4 muscles from abdominal segment 2 were acquired using a SP5 laser-scanning microscope (Leica Microsystems) with either a HC PL APO CS2 20x/0.75 (late third instars ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Cell Biology 2022Quote: ... 40 s) Quantifoil Cu 300 R2/2 grid and plunge frozen in liquid ethane with a GP2 (Leica Microsystems, Wetzlar, Germany) blotted from behind ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Developmental Biology 2023Quote: ... GFP images were obtained every 2 s with a x40 oil-immersive objective (HC PL FLUOTAR 40x/1.30 OIL 340 nm, Leica Microsystems CMS) used for CBs and a x10 objective (N PLAN 10x/0.25 achromatic corrected objective ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Plant Biology 2023Quote: The localisations of GFP-AGP and GFP-AGPΔC expressed in transformed BY-2 cells were visualized using a confocal laser scanning microscope (TCS SP8, Leica, Wetzlar, Germany) essentially as described (Tasaki et al ...
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bioRxiv - Plant Biology 2023Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Physiology 2023Quote: ... Paraffin-embedded liver sections (2 μm or 7 µm for Sirius-Red staining) were processed for IHC staining using a BOND-MAX system (Leica, Germany) as previously described 54 ...
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bioRxiv - Cell Biology 2023Quote: Isolated islets were kept in culture overnight and frozen with a Leica EMpact 2 or EM ICE high-pressure freezer (Leica Microsystems). They were kept in liquid nitrogen until freeze substitution ...
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bioRxiv - Molecular Biology 2023Quote: ... Each z-stack acquired tissue volume (2 µm spacing) with 200 Hz line frequency using LAS X (Leica Application Suite X).
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bioRxiv - Plant Biology 2024Quote: ... and Antirrhinum majus stained with SR2200 and TO-PRO-3 iodide or DAPI was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized using vaporized isoflurane (5% for induction, 1.5% for maintenance) and placed in a stereotactic frame (Leica, Angle 2). Opthalmic ointment was applied and rectal temperature and respiration rate were monitored throughout surgery ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Cell Biology 2019Quote: ... on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems). Sections were poststained with 2% uranyl acetate in water and lead citrate ...
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bioRxiv - Cell Biology 2020Quote: ... on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems). Sections were post-stained with 2% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems). Sections were post stained with 2% uranyl acetate in water and lead citrate ...
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bioRxiv - Cell Biology 2023Quote: ... on a Leica Ultracut UCT 6 ultramicrotome (Leica Microsystems). Sections were poststained with 2% uranyl acetate in water and lead citrate ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed and incubated in periodic acid (Leica 38016SS4A) followed by the Schiff Reagent (Fisher SS32-500).
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bioRxiv - Cancer Biology 2021Quote: Unstained sections from the study cases were heated for 30 minutes in Bond™ Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems) using Leica BOND-MAXTM (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was blocked in 2% agar and 200 µm thick coronal sections were cut using a vibratome (Leica VT 1000 S). The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... GFP fluorescence resulting from LanB2:GFP expression in Dilp2 neurons was imaged with a Leica TCS SP8 MP 2-photon microscope (Leica, Wetzlar, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and acute horizontal slices (310 μm thick) were prepared using a vibratome (5000mz-2, Campden Instruments Ltd.; or VT1200S, Leica Biosystems). Slices recovered for 30 min in ∼37°C oxygenated Ringer’s solution that was identical to the dissection solution except with lower Mg2+ concentrations (1 mM MgSO4 ...
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Cancer Biology 2019Quote: ... homogeneous and mixed spheroids were generated and imaged repeatedly over time (2-3 times per week) using brightfield microscopy (4x objective; 0.3 NA, Leica DM IRBE, Hamamatsu). Spheroid volumes were calculated using SpheroidSizer58.
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bioRxiv - Cell Biology 2021Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+ fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software (US ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Histological analysis of the 2 µm paraffin-embedded tissue sections was performed with the Bond Polymer Refine Detection Kit (Leica, Wetzlar, Germany) on a BondMax system (Leica) ...
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bioRxiv - Neuroscience 2021Quote: ... the cochlea was glued on a metal block with Loctite 401 and cut to 2 halves by a vibratome (VT1200S, Leica, Wetzlar, Germany) with FREQ index at 7 ...
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bioRxiv - Bioengineering 2022Quote: Following embedding within 2% agarose and fixation overnight samples were dehydrated and embedded in paraffin using an automatic tissue processor (ASP300; Leica, London, UK). All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...