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Citations for Leica :
301 - 350 of 1644 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
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bioRxiv - Neuroscience 2019Quote: Wide-field calcium imaging of Hydra was conducted at 2 Hz using a fluorescence dissecting microscope (Leica M165) equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC) ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
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bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... 2’,7’-dichlorofluorescein (DCF) fluorescence was visualized by confocal laser scanning microscopy (CLSM) (Leica TCS-SP5, Mannheim, Germany) with excitation/emission at 495 nm/525 nm.
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bioRxiv - Microbiology 2020Quote: ... 0.05 % uranyl acetate in acetone and placed in an AFS-2 freeze-substitution machine (Leica Microsystems, Wetzlar, Germany). Tissues were freeze-substituted at −90 °C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
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bioRxiv - Immunology 2020Quote: ... 2-photon laser scanning microscopy was performed with a LEICA SP5 inverted 5-channel confocal microscope (Leica Microsystems) equipped with a 25X water-immersion objective ...
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bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265 ...
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bioRxiv - Microbiology 2023Quote: ... was applied to glow-discharged Quantifoil R2/2 grids and vitrified with a GP2 plunge-freezing device (Leica).
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bioRxiv - Plant Biology 2023Quote: Roots fixed with 2% (v/v) formaldehyde in Tris were embedded in Cryo-Gel (Leica Biosystems, ID:39475237) and cut onto a cryostat (Leica CM1950 ...
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bioRxiv - Biophysics 2023Quote: ... FFPE tissue samples were cut using a ultramicrotome in 2 πm thick sections (RM2125 RTS, Leica Biosystems, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Physiology 2024Quote: ... and Mac-2-staining were imaged using a Leica DMi8 Inverted Fluorescent Microscope (Leica Microsystems, Buffalo Grove, IL). Histological quantifications were performed using Image J software (v1.52q ...
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bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-TTF1 (Nkx2.1) (1:100; Novocastra; Leica Biosystems NCL-L-TTF-1), mouse anti-NeuN (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... the bladder-chip was dehydrated in a graded alcohol series and dried by passing them through the supercritical point of carbon dioxide (CPD300, Leica Microsystems). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... the sample was dried using carbon dioxide critical point drying (Leica EM CPD 300 Critical Point Dryer, Leica microsystems, Wetzlar, Germany) at a slow speed setting and CO2 gas was exchanged out at slow heating for 18 cycles ...
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bioRxiv - Developmental Biology 2020Quote: ... at least 3 transverse slices cut with a vibratome (VT1000S, Leica) at different anterior-posterior levels representing anterior ...
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bioRxiv - Neuroscience 2019Quote: ... The hippocampal regions were loaded into 3 mm aluminium planchettes (Leica Cat# 1677141 for type A and 1677142 for type B ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2023Quote: ... and sections (3 µm) were cut using a microtome (LEICA RM2255). The sections were deparaffinized by bathing twice in xylene (for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were sectioned at 3 μm using a microtome (Leica, Germany) and the sections were stained with hematoxylin–eosin (HE ...
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bioRxiv - Systems Biology 2023Quote: ... we collected 3 10 µm sections using a Microtome (Leica Byostems). We then extracted RNA using Qiagen RNAse FFPE kit according to manufacturer instructions and evaluated the percentage of total RNA fragments longer than 200 nucleotides (DV200 ...
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bioRxiv - Plant Biology 2019Quote: Samples used in cell number and cell size quantifications were fixed (1% glutaraldehyde, 4% formaldehyde, 0.05M sodium phosphate, and after dehydration, embedded in Leica HistoResin ...
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bioRxiv - Cell Biology 2020Quote: ... SSCs were incubated with the labeled EVs for 1–4 h and visualized using fluorescent microscopy (Leica, Wetzlar, Germany)
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S ...
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bioRxiv - Microbiology 2020Quote: ... After immunolabelling the grids were embedded with a mixture of 50% methylcellulose 2% and 50% sucrose 2.3M and then vitrified by plunge freezing with EMGP plunge freezer (Leica) at 30 °C and 90% humidity.
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S ...
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bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
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bioRxiv - Developmental Biology 2021Quote: ... and photographed with an Aperio AT 2 Leica Scanner and an Aperio Scan Scope Console program (Leica, Wetzlar, Germany) available at the Northern Finland Biobank Borealis ...
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bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
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bioRxiv - Pathology 2022Quote: ... Super-resolution images were recorded on the confocal laser scanning microscopy LSM 980 equipped with Airyscan 2 (Leica, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Developmental Biology 2020Quote: Embryos or neural tube explants are fixed in 3.7% formaldehyde for 2 hours and sliced using a vibratome (Leica). Proteins were detected on 50 μm vibratome sections ...
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bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Collected aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system with Cy5 channel (Leica). CXCR4 co-staining was done after fixation using whole mount staining protocol described above.
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bioRxiv - Cell Biology 2019Quote: ... Cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI) and imaged with a Leica TCS SP5 Confocal Laser Scanning Microscope (Leica) using a 40X water immersion objective.
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bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
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bioRxiv - Cell Biology 2020Quote: ... then transferred to brass specimen carriers and high pressure frozen with an EMPACT 2 (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...