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Citations for Leica :
301 - 350 of 1436 citations for 4 5 5 5 Tetrafluoro 4 trifluoromethoxy pentan 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and cross-sectioned to a thickness of 5 µm using a Leica RM2025 microtome (Leica Microsystems, Germany). The obtained histological sections were initially subjected to topographic staining using the hematoxylin-eosin (H/E ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... at 37°C and with 5% CO2 on an inverted DMi8 stand microscope (Leica, Microsystems, Wetzlar, Germany) equipped with a Flash 4.0 sCMOS camera (Hamamatsu ...
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bioRxiv - Microbiology 2024Quote: ... Live-cell imaging was conducted using an automated inverted confocal laser scanning microscope (Leica Stellaris 5 Confocal). mCherry fluorescence was excited at 580 nm with a laser set to 5% power ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were air-dried for 5 min before loading onto a Bond Rx instrument (Leica Biosystems). Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No ...
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bioRxiv - Neuroscience 2024Quote: ... and Z-stacks with 0.12 µm spacing were obtained on the Stellaris 5 confocal microscope (Leica Microsystems) with a 63x or 100x oil objective ...
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bioRxiv - Microbiology 2024Quote: ... The stained dishes were visualized with an inverted confocal laser scanning microscope (CLSM) (Leica Stellaris 5, DMi8) using a 20× objective ...
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bioRxiv - Cancer Biology 2024Quote: ... Confocal images were collected using a TCS-SP 5 spectrophotometric multiphoton laser scanning confocal microscope (Leica Microsystems) collecting 0.2 - 1 µm optical sections.
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bioRxiv - Cell Biology 2024Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence microscope (Leica AM TIRF inverted microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were embedded in 5% agarose and sliced along the coronal axis at 50 µm (Leica, VT1200S).
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bioRxiv - Neuroscience 2024Quote: Vitrified samples were imaged at -195°C utilizing the STELLARIS 5 Cryo Confocal Light Microscope from Leica Microsystems ...
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bioRxiv - Cell Biology 2024Quote: ... z-stack images of CDMs and dCDMs were collected with a Leica TCS SP 5 (Leica Microsystems) combined with MPM in which the NIR femtosecond laser beam was a tunable compact mode-locked titanium:sapphire laser (Chameleon Compact OPO-Vis ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A series of time laps pictures was taken (Leica M50, 5 MP HD Microscope Camera Leica MC170 HD) every 10s for 24h ...
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bioRxiv - Cancer Biology 2022Quote: ... The specimen frozen blocks were serially sectioned longitudinally at respectively 5 μm thickness using Cryostat microtome (Leica CM1950). Sections were used for H&E and immunofluorescence staining ...
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bioRxiv - Developmental Biology 2022Quote: ... The tissues were then cut into 5 μm sections using a microtome (HistoCore BIOCUT, Leica Biosystems, Nussloch, Germany) and coated onto glass slides ...
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bioRxiv - Cell Biology 2022Quote: ... After subsequent freeze substitution (using acetone, containing 0.25% osmium tetroxide, 0.2% uranyl acetate, 0.05% ruthenium red, and 5% water) (Leica AFS2), cells were embedded in Epon812 substitute resin (Fluka) ...
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bioRxiv - Cell Biology 2022Quote: A minimum of 5 fields per cover slip were imaged using a SP-8 Lightning Confocal Microscope (Leica) or CSU-W1 Spinning-Disk Confocal Microscope (Nikon) ...
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bioRxiv - Pathology 2024Quote: Intimal hyperplasia: Paraffin cross sections (5 μm thick) were cut at equally spaced intervals using a microtome (Leica) and then stained (Hematoxylin and Eosin ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... The sample was then embedded in optimal cutting temperature compound (O.C.T., Tissue-Tek) and cryo-sectioned into 5 μm longitudinal sections (Leica). Slides were equilibrated in hybridization buffer (0.9 M NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Brains embedded in 5% agarose were cut into 500 μm-thick sections using a vibratome (Leica VT1220, Biosystems). Brain sections were clarified using Rapid Clear® CS mounting solution (SunJin Lab ...
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bioRxiv - Genomics 2024Quote: ... DAPI was applied for 5 min for nuclear counterstaining before visualization using confocal microscopy (Leica TCS SP5 MP). PLA was performed usin the DuolinkII fluorescence system (Olink Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μm (Arabidopsis and barley roots) or 5 μm (barley leaves) of cryosections cut by cryostat (Leica CM1850) were attached to selected polysine adhesion slides (PolysineTM adhesion microscope slides Cat.# 48382-117 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Developmental Biology 2023Quote: ... and tissues were sectioned at 5 micron thickness using a Leica microtome for downstream applications (Leica Biosystems, RM2155).
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bioRxiv - Immunology 2023Quote: ... the fetuses were embedded in paraffin and 5 μm thick sections were cut with a microtome (Leica Biosystems). Morphometric analyzes were performed on E18 and E21 slides ...
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bioRxiv - Physiology 2023Quote: ... Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems). Paraffin sections were melted at 60 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... The wafer was then coated with a 5 nm carbon layer using an EM ACE 600 (Leica Microsystems) to ensure conductivity ...
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bioRxiv - Biochemistry 2023Quote: ... tissue blocs were sectioned to 5 μm thickness on a rotary microtome (RM 2125RT Leica Microsystems, Wetzlar, Germany). Gallya’s silver staining was performed following the manufacturer’s protocol (Morphisto ...
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bioRxiv - Molecular Biology 2023Quote: ... cardiac specimens were cut into 5-µm-thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany) (15723985) ...
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bioRxiv - Cancer Biology 2024Quote: ... the 5-micron FFPE tonsil sections were dewaxed on BOND RX autostainer (Leica Biosystems, Buffalo Grove, IL, USA), and antigen retrieval were performed with Leica ER1 buffer (Cat# AR9961 ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips were mounted onto glass slides using Fluoromount-G and visualized with confocal microscope (LEICA STELLARIS 5). 3D stacks were acquired to visualize distribution of lipid droplets ...
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bioRxiv - Plant Biology 2024Quote: ... Calcium imaging was performed after 24 hours post-transfection using confocal laser scanning microscope (CLSM-Leica Stellaris 5) equipped with a 40x oil objective (NA = 1.30) ...
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bioRxiv - Plant Biology 2024Quote: ... the roots of 5-d-old seedlings were cryofixed with an HPM100 high-pressure freezer (Leica Microsystems, Austria) and incubated in freeze-substitution medium (anhydrous acetone with 0.25% glutaraldehyde and 0.1% uranyl acetate ...
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bioRxiv - Molecular Biology 2024Quote: ... containing the zygotes were placed under a confocal laser scanning microscope (Leica Stellaris 5 – Leica Microsystems, Wetzlar, Germany) and the corresponding software LAS X Life was utilized for analysis ...
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bioRxiv - Bioengineering 2024Quote: Cell viability and cell aspect ratio were determined from microscopy performed with a STELLARIS 5 confocal microscope (Leica) combining fluorescence and confocal reflectance imaging modalities with a 10X air objective or 40X oil immersion objective ...
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bioRxiv - Biophysics 2024Quote: ... the transparent 96-well cell culture plate was placed on the stage of confocal microscope (STELLARIS 5, Leica) and heated to 37°C with the Stage-top Incubator System T (Live Cell Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA overnight at 4°C and afterwards cut with a vibratome (Leica Microsystems, Wetzlar Germany) in 100 μM thick sequential sagittal slices ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed overnight at 4°C in 4% PFA (pH = 7.2) and 40-μm coronal sections were collected using a vibratome (VT1000S, Leica). After a 15-min incubation in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
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bioRxiv - Neuroscience 2021Quote: ... Spheres were allowed to grow for 5 weeks and were photographed using an inverted bright-field microscope (Leica DMIRBE). Viable spheres were recovered by centrifugation at 100g for 5min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day old Arabidopsis seedling roots expressing AtC53-mCherry were cut and high-pressure frozen (EM PACT2, Leica, Germany), prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20× ...