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Citations for Leica :
301 - 350 of 717 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Brains electroporated with the pCAG-mRFP;Hes5p-GFP plasmid were dissected 11 h after electroporation and embedded in 4% Agarose in complete HBSS medium (Polleux and Ghosh, 2002) and sectioned using a vibratome (Leica VT1200S). Electroporated areas of the lateral cortex were microdissected under the fluorescent dissecting scope (Zeiss Axio Zoom) ...
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bioRxiv - Immunology 2022Quote: ... grids were metalized by platinum sputtering (4 nm) using a Leica ACE600 cryo-sputter coater equipped with a cryo-stage (Leica Microsystems). Metallized samples were then transferred to a pre-cooled Crossbeam 550 FIB-SEM microscope (Carl Zeiss NTS GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused with 4% PFA in PBS and brains were removed and sectioned coronally or sagittally on vibratome (Leica VT1000) at 50-75 μm and mounted on slides with ProLong(tm ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde in PBS and imaged using laser scanning confocal microscopy (LEICA DMi8, Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed with 4% PFA in PB overnight and 50-µm coronal sections of the brainstem were obtained on a vibratome (Leica VT1200S). Expression of EGFP at the calyx of Held terminals was visualized using an epifluorescence inverted microscope (CKX41 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using an ACE600 cryo-sputter coater (Leica Microsystems GmbH) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
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bioRxiv - Microbiology 2023Quote: ... samples were stored light-protected in the same buffer at 4°C until imaging with a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) using sequential scanning mode.
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bioRxiv - Pathology 2023Quote: ... the specimens were paraffin-embedded and subsequently sectioned into 4-μm-thick tissue slices by a microtome (RM2235, Leica Microsystems Inc.). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...
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bioRxiv - Cell Biology 2023Quote: ... confocal microscope in 3 Z-stacks at 20x (1024x1024 resolution) and further imaged at 63x with immersion oil in 4 Z-stacks to highlight regions of interest (Leica Sp8x).
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bioRxiv - Neuroscience 2023Quote: ... Half brains were fixed in 4% paraformaldehyde for 24h and sectioned at 40 μm thickness in the coronal plane on a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains were post-fixed in 4% PFA overnight and sectioned coronally at 50 µm thickness (approximately 240 sections per brain) on a vibratome (Leica VT1200S). Brain sections containing regions of interest were permeabilized in 1% Triton X-100 in TBS and blocked in 5% normal goat serum ...
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bioRxiv - Cancer Biology 2024Quote: ... immunostaining of formalin-fixed paraffin-embedded sections (2-4 μm) was performed with the Bond RX automated stainer (Leica Biosystems, Germany). Antigen retrieval was 20 mins at 100°C using the Bond Epitope Retrieval solution 2 (EDTA based buffer ...
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bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA at 4 °C and then rinsed in PBS before being sectioned at a thickness of 60 µm using a vibratome (Leica Biosystems). These sections were then mounted onto Superfrost Plus slides (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and LHA (for recordings) were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000, Ted Pella, or VT1000P, Leica). Sections were collected into cryoprotectant (25% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature, incubated with 20% sucrose (Fisher BioReagents, BP220-1) overnight at 4°C and then embedded in OCT (Leica, 14020108926) and stored at −80°C until further use.
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C with the following dilutions in blocking buffer: p53 (CM5) (1:300, NCL-L-p53-CM5p, Leica Biosystems), Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were transferred to 30% Sucrose solution overnight at 4°C before OCT (Sakura) inclusion for performing 20 µm coronal sections using Cryostat (Leica Biosystems). Cryostat sections were washed three times with PBST (1x PBS with Tween 20 ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks were warmed to −20°C for at least 1h prior to sectioning and sectioned at 18 µm using a cryostat (Leica CM3050S) at −20°C ...
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bioRxiv - Bioengineering 2022Quote: ... 3′-diaminobenzidine (Leica).
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...