Labshake search
Citations for Leica :
301 - 350 of 813 citations for 4 2 Trimethylsilyl Ethyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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bioRxiv - Plant Biology 2021Quote: ... Observations were carried out every 2–3 days with an electronic binocular microscope (Leica M80) to monitor seed germination ...
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bioRxiv - Cancer Biology 2022Quote: ... and CD8 alpha antigen retrieval was performed with BOND Epitope Retrieval 2 – ER2 (AR9640, Leica) for 40 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μm z-steps) were taken using a laser scanning confocal microscope (TCS SP8, Leica). Three sections of 20 μm depth each were averaged per animal for analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system (Leica).
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bioRxiv - Cell Biology 2020Quote: ... L4 animals were fixed by high-pressure freezing with the EMPACT-2 system (Leica Microsystems). Freeze-substitution (FS ...
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bioRxiv - Immunology 2021Quote: ... samples underwent heat induced antigen retrieval (HIER) in BOND Epitope Retrieval 2 (AR9640, Leica Biosystems) or BOND Epitope Retrieval 1 (AR9961 ...
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bioRxiv - Plant Biology 2023Quote: ... and images were taken every 20 min for 2 days using microscope (LEICA M205 FA). Imaging data were analyzed with ImagJ (FIJI).
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bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by an initial antigen retrieval using Epitope retrieval solution 2 (Leica Bond ER2, #AR9640) at 95°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Freeze substitution was performed in a Leica EM AFS-2 freeze substitution unit (Leica Microsystems) as described previously (Bragulat-Teixidor et al. ...
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bioRxiv - Immunology 2024Quote: ... Heating mediated-epitope/antigen retrieval was performed with Bond TM Epitope Retrieval 2 (Leica Biosystems). Blocking was performed with plant-based protein blocking buffer (Cell Signaling Technology [CST] ...
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bioRxiv - Bioengineering 2022Quote: ... and the appropriate visualization system was applied – DAKO DAB (Cat# K346811-2, Leica, Heidelberg, Germany) for all but CD206 which utilized AEC (Cat# K400511-2 ...
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bioRxiv - Developmental Biology 2024Quote: ... the stained larvae were mounted in 2 % methylcellulose and photographed by a fluorescent stereomicroscope (Leica) or mounted in low-melting agarose gel and photographed by a confocal microscope (Olympus FV1000).
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bioRxiv - Cancer Biology 2024Quote: ... for transferrin receptor 1 (TfR1) and using Bond Epitope Retrieval Solution 2 (Leica Biosystems AR9640) for Ki67 and CD31 ...
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bioRxiv - Biochemistry 2024Quote: ... Grids were blotted for 2 s with an EM GP2 Plunge Freezing device (Leica Microsystems) held at 4 °C and 90% humidity before freezing in liquid ethane ...
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bioRxiv - Biophysics 2021Quote: ... and incubated for 10 s prior to blotting on Whatman 1 paper for 4 s and plunge-frozen in liquid ethane using a GP2 plunge freezer (Leica) equilibrated to 10°C and 85% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agar (3-4%) and coronal sections (50 µm) were cut on a vibrating microtome (Leica VT1000S) and collected in a 1 in 4 series ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Evolutionary Biology 2021Quote: ... 300µm-thick acute coronal brain sections were cut after embedding in 4% low melt-agar using a vibratome (Leica, #VT1000S) under RNase-free conditions ...
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5% glutaraldehyde and 4% paraformaldehyde in buffer solution) removed and coronally cut into 350 µm thick sections using a Vibratome (Leica VT1000 S ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Neuroscience 2020Quote: ... the slices were embedded in 4% low melting agarose and cut in 70µm thick sections in a vibrating microtome (Leica, VT1000S). Sections around the tip of the recording electrode were collected and cryoprotected in 10% and 20% sucrose solutions in PB ...
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bioRxiv - Physiology 2021Quote: ... then individually embedded in paraffin blocks and sectioned in serial sagittal sections (4 μm) using a rotary microtome (Leica, Germany). Tissue sections were permanently mounted ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Color-coded time projections were generated in ImageJ using a publicly available plugin (K ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 20 µl of the protoplast suspension were pipetted to 200 µL high osmolarity solution (Supplementary Table 4) in a perfusion chamber mounted on the stage of an inverted microscope (DMi8, Leica). Protoplasts were allowed to settle down for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TauRDP301S-CFP/YFP and HEK293 TauRDP301S-CFP cells treated with Tau condensates were fixed with 4% PFA in PBS prior to confocal FLIM on a Stellaris 8 FALCON (Leica) equipped with a 100x objective ...
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bioRxiv - Genomics 2022Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Microbiology 2022Quote: ... the eyes were cut into 4 μm sections and stained with H&E (BBC Biochemical) using an autostainer (ST5010, Leica). Images were obtained using an Olympus BX51 microscope (Olympus ...
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bioRxiv - Developmental Biology 2020Quote: ... images were taken as a time series on an xy plane across 6 z planes at an interval of 4 s using a 10× objective with an NA of 0.4 and an optical zoom of 4 on an SP5 inverted confocal microscope mounted with a resonant scanner (Leica Microsystems). The z project across time was then obtained as a time series.
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bioRxiv - Neuroscience 2020Quote: ... One hemisphere was post-fixed by incubation for 48 h in 4% paraformaldehyde (PFA) and cut into 35 μm sections with a vibratome (Leica) for histological analyses (brains used for RNAscope were perfused with both PBS and 4% PFA prior post-fixation and cut in 10 μm sections with a cryostat) ...
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bioRxiv - Cell Biology 2021Quote: ... Dried samples were then coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The sample grids were imaged using a JEOL JEM2800 scanning/transmission electron microscope at 200 kV.
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bioRxiv - Cell Biology 2021Quote: ... brains fixed in 4% paraformaldehyde (PFA) were embedded in paraffin and sectioned 5µm thick using a microtome system (Leica RM2165). Immunohistochemistry was performed on brain sections by permeabilizing them with TBS-0.5% Triton-X (TBS-Tx ...
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bioRxiv - Physiology 2021Quote: ... tissue was fixed in 4% buffered paraformaldehyde and samples subsequently paraffin-embedded and 5 μm sections prepared on a microtome (Leica). Adipose sections were viewed at 20 x magnification ...
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bioRxiv - Cancer Biology 2020Quote: Paraffin tissue-blocks were serially sectioned with slices thickness of 4 µm on a microtome (Leica Byosystem, Buffalo Grove, IL) and allowed to dry at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed in 4% PFA overnight and coronally sectioned at 75 µm on a Vibratome (Leica VT1000 S). All slices were then mounted on Superfrost Plus slides using Fluoromount-G mounting medium (Southern Biotech ...
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bioRxiv - Plant Biology 2020Quote: Seventy µm-thick transversal sections of the midrib of leaflets 4 or 5 from L1 leaf were cut using a vibratome (Leica) and stained with periodic acid (1% w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum containing hNSCs (equivalent to +1.4 to +0.14 mm from Bregma) (Franklin and Paxinos, 2007) was cut at 60 μm using a vibratome (Leica Microsystems). After pre-embed IHC of the striatum using diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed heads were embedded in 4% low gelling agarose and 200 µm sections were obtained using a vibratome (Leica, VT1200S). Sections were post-fixed with 1% osmium tetroxide in water ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Neuroscience 2021Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell aggregates and transplanted retinas harvested from RD-nude rats were fixed with 4% paraformaldehyde (Wako) and sectioned with a cryostat (Leica) to prepare frozen sections ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...