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Citations for Leica :
251 - 300 of 327 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal 20μm-think brain sections that contained the barrel cortex were cut and collected on-slide (wS1) using a cryostat (Leica, CM3050 S). The slides were then stored at −80°C until further processing ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were cut into 50 μm slices, images captured using Carl Zeiss Axio Scan microscope (Promenade 10, Jena, Germany) or confocal scanning microscope (DMi8, Leica, Wetzlar, Germany).
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bioRxiv - Bioengineering 2022Quote: ... The scaffolds were then stored in 0.15 M NaCl until imaging using a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective.
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bioRxiv - Cell Biology 2024Quote: z-stacks (0.2 to 0.4μm distance between planes) of immunolabelled cells and groups of cells were acquired using an inverted scanning confocal microscope (Leica SP5-SMD), 63x oil objective (1.4NA) ...
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bioRxiv - Neuroscience 2024Quote: ... brains were washed with 0.1 M PBS and free-floating 30 µm coronal sections containing BLAa were cut on a vibratome (Leica VT 1200S, Leica Biosystems, USA). Sections were stored in anti-freeze solution (30% ethylene glycol ...
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bioRxiv - Plant Biology 2021Quote: ... The cryo-sections were placed onto the PET membrane of metal-frame slides (Leica, USA), and dehydrated using 99.5% ethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells that adhered to the membranes were imaged under a conventional microscope (DMI3000B, Leica) and counted manually.
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bioRxiv - Neuroscience 2023Quote: ... Sections were thaw-mounted onto PEN-membrane slides (11505158, Leica Microsystems, Buffalo Grove, IL, USA) and kept frozen until the next step ...
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bioRxiv - Genomics 2024Quote: ... and transferred to UV-irradiated PEN membrane steel frame slides 4.0 µm (Leica Microsystems, 11600289) for laser microdissection ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes from the inserts were excised and imaged using a fluorescence microscope (Leica DM4000B). The number of invaded cells across the entire membrane was counted ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Pathology 2021Quote: ... was performed on 5µm-thick sections of formalin-fixed lung mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... 1.0 mm deep with a controlled velocity of 5.0+0.2 m/s and a dwell time of 100 ms using a stereotaxic electromagnetic impactor (Impact one, Leica; Buffalo Grove, IL, USA) equipped with a 5.0 mm flat steel tip ...
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bioRxiv - Genetics 2020Quote: ... Serial cryosections (10 μm for embryos and 14 μm for larvae and adult zebrafish) were obtained in a Leica CM3050 S cryostat (Leica Ltd., Wetzlar, Germany) and stored at −20 °C for further use.
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bioRxiv - Neuroscience 2022Quote: Temporal lobe tissue fixed with 2.5% glutaraldehyde-2% PFA in 0.1 M phosphate buffer (PB) was transversely sectioned at 200 µm using a Leica VT1200S vibratome (Leica Microsystems GmbH, Heidelberg, Germany). Slices were further post-fixed in 2% osmium tetroxide in 0.1 M PB for 1.5 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 400 μm thick horizontal slices containing the cortex and hippocampus were cut with a vibratome (OTS-4500, Electron Microscopy Sciences /Leica VT1000s, Leica Biosystem) under ice-cold ACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Four-µm-thick FFPE sections were incubated with the antibodies listed in Supplementary Table S1 on a BondTM Automated System (Leica Microsystems, Wetzlar, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Each brain slice was melted on a steel frame PEN-membrane slide (Leica microsystems, Cat #11600289) pre-coated with Poly-L-Lysine (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: 4 μm thick FFPE sections were mounted on polyethylene naphthalate membrane (PEN) slides (Leica cat. 11600289) previously UV-photoactivated in an UV crosslinker for 30 min (BLX-254 ...
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bioRxiv - Bioengineering 2023Quote: ... 10 images from different regions of the membrane were acquired with a fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera).
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bioRxiv - Neuroscience 2024Quote: ... while the other one was placed on a membrane slide 2.0 μm PEN (Leica Microsystems, #11505158), air-dried for 30 minutes and stained with hematoxylin before dehydration in ascending (70% ...
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bioRxiv - Cell Biology 2020Quote: ... Small aliquots of about 2 μl were transferred to gold-coated copper planchettes (Engineering Office M. Wohlwend GmbH; Sennwald, Switzerland) and immobilized by high pressure freezing in a Leica HPM100 (Leica Microsystems GmbH, Wetzlar, Germany). The obtained frozen cell pellets were kept in liquid nitrogen until further use.
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bioRxiv - Cell Biology 2020Quote: ... The fresh frozen brain samples were sectioned sagittally at 14 μm thickness and the frozen brain homogenates at 20 μm thickness with a Leica CM3050 cryostat at −20°C (Leica Microsystems GmbH, Wentzler, Germany). All brain specimens were cut at approximately the same Bregma in order to clearly delineate the hippocampus ...
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bioRxiv - Neuroscience 2021Quote: ... followed by30% sucrose solution in PBS before tissue was sliced at a thickness of 40□m on a freezing microtome (Leica Biosystems, Illinois, United States).
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bioRxiv - Neuroscience 2022Quote: ... and examined using a Leica TCS SPE-II confocal microscope (confocal is Leica TCS SPE-II and a fluorescent is Leica DM4000 B/M LED). Two or three slices containing the PVN were collected from each specimen ...
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bioRxiv - Immunology 2023Quote: ... Frozen brains were then embedded in Epredia™ M-1 Embedding Matrix (Thermo Ficher Scientific) and cut in coronal sections using a cryostat (Leica CM3050S, Mannheim, Germany), and mounted on slides for either fluorescence immunocytochemistry (IF ...
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Neuroscience 2024Quote: ... brains were washed with 0.1 M PBS and free-floating 30 µm coronal sections containing BLAa were cut on a vibratome (Leica VT 1200S, Leica Biosystems, USA). Sections were stored in anti-freeze solution (30% ethylene glycol ...
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bioRxiv - Bioengineering 2022Quote: ... secondary and myofiber membranes were identified with anti-α-sarcoglycan antibody (Leica Biosystems, Wetzlar, Germany, 1:200) with an Alexa Fluor™ 568 secondary (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: The 5.0-µm FFPE sections for laser microdissection (LMD) were mounted onto PEN-membrane glass slides (Leica) following by deparaffinization ...
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bioRxiv - Cell Biology 2023Quote: ... tympanic membrane and ossicles were carefully removed under a stereomicroscope (Leica M165 FC, Leica Biosystems, Wetzlar, Germany). To perfuse buffer through the middle ear ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Sections were cut at 20–25 µm on a cryotome and attached to membrane slides (Leica, 11505189) with a 2 µm PEN-Membrane ...
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bioRxiv - Biophysics 2024Quote: ... Three heads at a time were transferred with a cut pipette tip into high-pressure freezing carriers made from aluminum (Leica Microsystems or M. Wohlwend GmbH) with 200μm depth ...
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bioRxiv - Genomics 2024Quote: ... Sections to be processed in the Laser-Capture microdissection workflow were collected on PEN membrane glass slides (Leica) and processed/stored similarly.
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bioRxiv - Cancer Biology 2020Quote: ... The tissue blocks were sectioned by cryotome into 170 - 210 consecutive 10 µm thin tissue sections (1.7 - 2.1 mm total depth) which were placed on PEN membrane slides (Leica Microsystems). Representative sections were mounted on charged glass slides and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2022Quote: ... Lamella sites were chosen based on the cell membrane signal obtained from the DM6 cryo-Light microscope (Leica Microsystems) correlated with the SEM images using MAPS 3.14 software ...
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bioRxiv - Developmental Biology 2022Quote: ... 15μm brain sections were cut and adhered to PEN membrane-coated glass slides (Leica Microsystems Inc, Buffalo Grove, IL) and frozen at −80°C ...
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bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides ...
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bioRxiv - Neuroscience 2020Quote: ... with one to three processes and a smooth cell membrane (Fig 2A) as visualized using an inverted microscope (DMIL; Leica) equipped with a fluorescent LED light source (X-Cite 110LED ...
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bioRxiv - Neuroscience 2021Quote: ... 20µm sections with alignment holes punched into the tissue by a glass pipet were prepared in a series with every other section aimed for in situ hybridization and collected on superfrost plus slides and the other for laser capture microdissection and collected on PEN coated membrane slides (Leica). Double fluorescence in situ hybridization (FISH ...
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bioRxiv - Developmental Biology 2020Quote: ... For sequence analysis fetal tissues embedded in OCT were sectioned at 10µm onto polyethylene napthalate (PEN) membrane slides (Leica, 11505158) and heart tissue was collected using a Leica LMD7000 Laser Microdissection System from CVB3-infected fetuses showing a VSD and uninfected control fetuses ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µm per z-step) of neurons expressing fluorescent membrane markers were captured using a 20×0.7 NA (Leica SP5) 20×0.75 NA (Leica Stellaris ...
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bioRxiv - Neuroscience 2024Quote: ... and we determined the distribution of GM1 on the lipid structures membrane using a confocal microscope (LEICA SP8 STED 3X).
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bioRxiv - Physiology 2024Quote: ... The number of neutrophils that migrated to the lower side of the membrane was counted in 10 random microscope fields using the x20 objective (Leica). Neutrophils that passed through the membrane were quantified ...