Labshake search
Citations for Leica :
251 - 271 of 271 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: In situ hybridization was performed on 16 μm thick midbrain slices from a fresh-frozen mouse brain cut on a cryostat (Leica CM3050 S). All FISH reagents used are commercially available from ACD bio ...
-
bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
-
bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
-
bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested from the Inbred immunocompetent & NOD.SCID treatment study mice were stained for Cm major outer membrane protein (MOMP) using an automated staining platform (Leica Bond RX, Leica Biosystems). After deparaffinization and heat-induced epitope retrieval in citrate buffer at pH 6.0 ...
-
bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
-
bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
-
bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
-
bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
-
bioRxiv - Molecular Biology 2024Quote: ... Mouse plantaris muscles preserved for IHC were sectioned at a thickness of 7 μm using a cryotome (Leica Biosystems; Buffalo Grove, IL, USA) and adhered to multiple positively charged slides for the interrogation of various targets as explained in this and subsequent paragraphs ...
-
bioRxiv - Cell Biology 2024Quote: ... Fresh-frozen wildtype mouse kidneys were embedded in OCT (Sakura) and sectioned at 10 µm using a rotational cryomicrotome (HM500 Leica Microsystems, Wetzlar, Germany) and collected on super frost slides ...
-
bioRxiv - Bioengineering 2024Quote: ... Morphology of the collagen fibers in aged and young mouse breast tissues and tail tendons were visualized through second harmonic imaging (SHG) with a two-photon microscope (Leica Stellaris 8 Dive). SHG imaging was performed at 25x magnification with optical zoom on the specific sites.
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
-
bioRxiv - Molecular Biology 2021Quote: Facet joint cartilage and synovium (containing a thin layer of fibrous capsular tissue due to technical difficulties in obtaining pure synovium from mouse facet joints) were freshly dissected under a microscope (LEICA M80, with a LEICA IC80 HD video camera). Cartilage and synovium samples were collected separately in RNAlater solution (Ambion ...
-
bioRxiv - Physiology 2021Quote: ... Fast red detection of mouse Rxfp4 was performed on the Bond Rx using the Bond Polymer Refine Red Detection Kit (Leica Biosystems, Cat No. DS9390) with an incubation time of 20 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...
-
bioRxiv - Neuroscience 2024Quote: ... The frame was linked to a digital mouse brain atlas to guide the targeting of different brain structures (Angle Two Stereotaxic System, Leica Biosystems Division of Leica). The following stereotaxic coordinates in anteroposterior axis (AP) ...
-
bioRxiv - Molecular Biology 2021Quote: Facet joint cartilage and synovium (containing a thin layer of fibrous capsular tissue due to technical difficulties in obtaining pure synovium from mouse facet joints) were freshly dissected under a microscope (LEICA M80, with a LEICA IC80 HD video camera). Cartilage and synovium samples were collected separately in RNAlater solution (Ambion ...