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Citations for Leica :
251 - 300 of 399 citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the Bond Polymer Refine Detection Kit (DS9800, Leica). The slides were scanned with a whole-slide scanner (Aperio AT2 ...
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bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2021Quote: ... and coronal sections of the mouse brain containing the dentate gyrus and prefrontal cortex were then cut using a VT1200S microslicer (300 μm) (Leica, Wetzlar, Germany). Micro-dissected areas were post-fixed at 4C for 2 hours in 2% osmium tetroxide in 0.1mol/l Cacodylate Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The left and right hippocampus was micro dissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) and placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany). Mice were given a subcutaneous injection of meloxicam (2 mg/kg ...
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bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
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bioRxiv - Plant Biology 2019Quote: For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
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bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
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bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were agroinfiltrated to transiently express the protein of interest and examined with a Leica TCS SP5 Laser Scanning Confocal Microscope (Leica Microsystems, Exton, PA, USA) using a 63x water immersion objective NA 1.2 (HCX PL APO) ...
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bioRxiv - Immunology 2022Quote: ... The antibody was detected using Bond Polymer Refine Detection kit (Leica Biosystems) with diaminobenzidine (DAB ...
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bioRxiv - Physiology 2020Quote: ... and staining using the BondTM Polymer Refine Detection kit (DS9800, Leica Biosystems). Brightfield images were acquired using a Leica DM 1000 LED microscope and processed using Leica Application Suite software (Leica Biosystems).
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bioRxiv - Cancer Biology 2020Quote: ... before peroxidase block was performed using an Intense R kit (Leica, DS9263). Caspase 3 was stained at a previously optimised dilution of 1/500 and Ki67 at 1/100 before washing with Leica wash buffer and then application of rabbit envision secondary (Agligent ...
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bioRxiv - Immunology 2022Quote: ... Slides were counterstained with hematoxylin (Bond Polymer Refine Detection Kit, DS9800, Leica Biosystems.
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bioRxiv - Cancer Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 minutes ...
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bioRxiv - Pathology 2021Quote: ... with the Bond Polymer Refine Kit (cat. DS9800 Leica Biosystem, Richmond, IL). Immunostaining was performed using the Bond RX automated system (Leica Biosystems ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... the slides were stained with the Bond Refine Detection Kit (Leica Biosystems), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... For the IHC portion a Bond Refine staining kit (Leica Biosystems, DS9800) was used with a standard protocol minus the peroxide blocking step which was deleted ...
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bioRxiv - Cell Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). FFPE sections were rinsed with wash buffer and Podxl antibody applied for 30 minutes (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... and detected with DAB using the HRP Polymer Refine Detection Kit (Leica) and hematoxylin counterstaining ...
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bioRxiv - Cancer Biology 2024Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 min ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed using the Bond Polymer Refine Kit (DS9800, Leica Biosystems). For dual staining ...
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bioRxiv - Cancer Biology 2023Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica). Sections for p21 had blocking solution applied from the Rat ImmPRESS kit (MP-7404 ...
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bioRxiv - Biophysics 2024Quote: ... with a bond polymer refine detection kit (Leica Biosystems, Milton Keynes,UK), and 3,3’-diaminobenzadine as a substrate ...
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bioRxiv - Molecular Biology 2021Quote: Facet joint cartilage and synovium (containing a thin layer of fibrous capsular tissue due to technical difficulties in obtaining pure synovium from mouse facet joints) were freshly dissected under a microscope (LEICA M80, with a LEICA IC80 HD video camera). Cartilage and synovium samples were collected separately in RNAlater solution (Ambion ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Pathology 2019Quote: ... Secondary detection was performed this the Bond Polymer Refine Detection Kit (Leica, DS9800). Tissue sections were imaged using a Nikon Eclipse Ci light microscope with 4x or 20x objectives and Nikon Elements software.
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bioRxiv - Cancer Biology 2020Quote: ... Diaminobenzidine (DAB) staining was developed using the Bond Polymer Refine Detection Kit (Leica). Slides were counterstained with hematoxylin ...
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bioRxiv - Cancer Biology 2022Quote: ... blocking of endogenous peroxidase activity with Bond polymer refine detection kit (Leica: DS9800) (iii ...
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bioRxiv - Microbiology 2022Quote: ... Detection was completed using the Bond Research Detection kit (Leica Biosystems; CAT# DS9455). Nuclei were counterstained with DAPI (Vector Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... 306C7B3 stainings were done with enzyme pretreatment (Bond Enzyme Pretreatment Kit, Leica Biosystems) at 37°C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... blocking of endogenous peroxidase activity with Bond polymer refine detection kit (Leica: DS9800) (iii ...