Labshake search
Citations for Leica :
251 - 300 of 501 citations for Goat Anti Rabbit IgG HRP H+L since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... filled with PBS-BSA (1%) and imaged with 20× (HCX PL Fluo TAR L, 0.40, dry, Leica), 40× (HCX PL Fluo TAR L ...
-
bioRxiv - Genetics 2021Quote: ... were ruled out by a monoclonal antibody against α-synuclein (NCL-L-ASYN; Leica Biosystems, Wetzlar, Germany). The staging of AD was performed by using the ABC score according to the updated National Institute on Aging-Alzheimer’s Association guidelines (50) ...
-
bioRxiv - Plant Biology 2023Quote: ... Leica DMI6000B equipped with a Leica N Plan L 20×/0.4 CORR PH1 objective (Leica, Wetzlar, Germany) and a Hamamatsu Orca camera (model C4742-80-12AG ...
-
bioRxiv - Neuroscience 2022Quote: Images were acquired using a Leica TCS SP8 confocal microscope equipped with a 25X CLARITY objective (6.0-mm working distance; HC FLUOTAR L 259/1.00 IMM; ne = 1.457; motCORR VISIR; Leica) designed to match the 1.45 refractive index of cleared hybridized tissue ...
-
bioRxiv - Cell Biology 2022Quote: ... The staining was examined using HRP Envision Systems (DAB Kit, MXB Biotechnologies) and analyzed using a dissecting microscope (Leica DM4000).
-
bioRxiv - Biochemistry 2020Quote: ... Slides were prepared following usual H&E staining post parafilm block preparation and tissue sectioning using manual microtome machine (Leica RM2235 Manual Rotary Microtome for Routine Sectioning) ...
-
bioRxiv - Cancer Biology 2020Quote: ... slides were stained with hematoxylin and eosin (H&E) and visualized by bright field microscopy at 4X microscopic magnification using Leica DMI-6000B (software: Leica Application Suite X (Leica Microsystems ...
-
bioRxiv - Plant Biology 2020Quote: ... The tobacco plants were kept in the dark for 48 h after infiltration and then GFP fluorescence was observed under a confocal microscope (TCS SP8, Leica). Pro35S:GFP was used as a control ...
-
bioRxiv - Neuroscience 2022Quote: ... 11506243 were taken in 20 min intervals for 67 h with a DMC2900 color camera (both Leica Microsystems, Wetzlar, Germany), with cells kept at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... the eyes were cut into 4 μm sections and stained with H&E (BBC Biochemical) using an autostainer (ST5010, Leica). Images were obtained using an Olympus BX51 microscope (Olympus ...
-
bioRxiv - Pathology 2021Quote: ... The tissue was H&E-stained and imaged with a bright-field microscope scanner (Aperio Whole Slide Scanner, Leica Biosystems) for reference ...
-
bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
-
bioRxiv - Cell Biology 2019Quote: ... Single-cell tracks were acquired using live-cell imaging at 10 min intervals over 24 h (AS MDW live cell imaging system, Leica). Multiple position imaging was gathered using Image Pro 6.3 (Media Cybernetics Ltd ...
-
bioRxiv - Neuroscience 2020Quote: ... One hemisphere was post-fixed by incubation for 48 h in 4% paraformaldehyde (PFA) and cut into 35 μm sections with a vibratome (Leica) for histological analyses (brains used for RNAscope were perfused with both PBS and 4% PFA prior post-fixation and cut in 10 μm sections with a cryostat) ...
-
bioRxiv - Immunology 2022Quote: ... in a blinded manner on 1 µm thick microtome sections of paraffin-embedded whole-lungs stained with H&E and scanned at a 40x magnification using a bright-field scanner (Aperio AT2, Leica). Morphological changes and immune cell infiltration in the lungs were graded as previously described (Li et al ...
-
bioRxiv - Physiology 2022Quote: ... eight images were randomly captured at 20X magnification along the length of an H&E stained SI section from each dam (Leica DMIL LED inverted microscope ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 33° C for the for at least 24 h before being imaged with a fluorescence on a microscope (Leica).
-
bioRxiv - Microbiology 2021Quote: ... Images were acquired every 15 min for 16 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL APO 63x/1.4 Oil ph3 objective Leica) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 μm sections were taken for H&E staining using standard methods and imaged using a DMI 6000B microscope (Leica). To examine tdTomato expression in sections ...
-
bioRxiv - Cancer Biology 2022Quote: Hematoxylin and Eosin (H&E) staining was performed on 5 μm thick slices using an automated system (Leica Autostainer, ST5010) with the protocol as described in Supplemental Table 1 ...
-
bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
-
bioRxiv - Microbiology 2022Quote: ... and one-way repeated measures ANOVA (Figs. 1D–F, and 2C-H). Syncytia sizes (Figs. 3B, 4B, and 4D) were quantified by Leica Applications Suit X (Wetzlar ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Time-lapse microscopy of developing genitalia was performed at 20 – 25 h after the puparium had formed on a DMI8 inverted microscope (Leica), equipped with a spinning-disc head CSU-W1 (Yokogawa Corporation of America ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
-
bioRxiv - Biochemistry 2023Quote: ... The H-E-stained tissue sections were visualized and exported from Aperio ImageScope (version 12.4.6, Leica Biosystems, Deer Park, IL).
-
bioRxiv - Immunology 2023Quote: ... Samples were hardened for 48 h and ultrathin sections were collected using a Leica UC6 ultramicrotome (Leica Microsystems, Wetzlar, Germany). The sections were imaged at 80 kV in Hitachi 7800 microscope (Hitachi High-Tech in America ...
-
bioRxiv - Bioengineering 2023Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
-
bioRxiv - Plant Biology 2023Quote: ... GFP signals were detected at different time intervals between 48-96 h post infiltration (hpi) with the TCS SP8 microscope (Leica).
-
bioRxiv - Neuroscience 2023Quote: ... was cryoprotected with 30% sucrose in PBS 0.1 M for 48 h and cut afterwards in 50-µm-thick coronal sections using a sliding microtome (LEICA SM2000R, Leica). Slices were collected in 6 subseries and stored at −20°C in a cryoprotective solution (30% glycerol ...
-
bioRxiv - Physiology 2024Quote: ... The images of the H&E stained pathology slides were taken using a digital pathology slide scanner (Leica, Aperio CS2) and analyzed by one of us (Dr ...
-
bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
-
bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
-
bioRxiv - Developmental Biology 2021Quote: ... Water immersion 10× objectives were used in both systems: N-Achroplan 10×/0.30 NA (Zeiss) and HC APO L 10×/0.30 NA (Leica), respectively ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using p53 polyclonal antibodies (NCL-L-p53-CM5p; Leica Biosystems). ChIPs were performed essentially as described previously (Kenzelmann Broz et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... The microscope was equipped with a WPlan-Apochromat 40× 1.0 NA (Zeiss) or HC Fluotar L 25x 0.95 NA (Leica) objective and controlled by ScanImage 2017b (Vidrio) ...
-
bioRxiv - Molecular Biology 2020Quote: Laser powers used were measured behind the objective lens (N PLAN L 20s/0.40, Leica Microsystems, Wetzlar, Germany) with a LabMax-TO laser power meter equipped with an LM-2 VIS sensor (Coherent ...
-
bioRxiv - Developmental Biology 2019Quote: ... Images were acquired bidirectionally with a low-magnification water immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). For time lapse ...
-
bioRxiv - Neuroscience 2020Quote: ... was included in the internal solution and cells were bolus-loaded for 5 min and patched-off by gently retracting the patch pipette.The fluorescence signal was captured on a Leica DMLFSA confocal microscope with a 63x water-immersion objective (NA = 0.9, HCX APO L 63x/0.9 W U-V-I, Leica) using the Leica Confocal Software (Version 2.61) ...
-
bioRxiv - Biophysics 2023Quote: Widefield fluorescence images were acquired using 20X air objective (HC PL FLUOTAR L PH1 20x, NA=0.4, Leica) mounted on a Leica DMi8 microscope ...
-
bioRxiv - Neuroscience 2024Quote: ... The output was relayed to the back focal plane of the objective lens (HCX IRAPO L 25×, 0.95 NA W, 2.5 mm WD, Leica) through the scan lens (LSM54-1050 ...
-
bioRxiv - Cell Biology 2020Quote: ... Automated freeze-substitution in 0.1% uranyl acetate in acetone over a 72 h period and embedding in Lowicryl HM20 resin were performed using an EM AFS2 (Leica Microsystems) as previously described (Kukulski et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
-
bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sections of TA and liver were stained with hematoxylin and eosin (H&E) using an XL CV5030 autostainer (Leica, Wertzler, Germany). Samples stained with H&E were scanned with a MIRAX MIDI digital slide scanner (Zeiss ...
-
bioRxiv - Microbiology 2022Quote: ... and were stained with hematoxylin and eosin (H&E) and coverslipped using a ST5010-CV5030 stainer/coverslipper combination (Leica, Wetzlar, Germany). Slides were examined blinded by a board-certified veterinary pathologist (N.A.C.) ...
-
bioRxiv - Biochemistry 2022Quote: ... 36 h post-transfection both the cell lines were trysinised and co-cultured in 1:1 ratio for 12 h followed by fixing permialization and subsequent imaging using fluorescence microscope (Leica Microsystems) and subsequent analysis by ImageJ software.
-
bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Bioengineering 2022Quote: ... The gels were subsequently incubated with EM containing 10 μM forskolin (Wako) with DMSO or Inh-172 for 24 h before imaging with a Leica DMi8 (Leica Microsystems) using the Leica Application Suite X (LAS-X ...
-
bioRxiv - Cancer Biology 2022Quote: ... spheres were incubated in n-Butanol (three times 30 min) and melted paraffin (three times) before they were Paraffin-embedded using the HistoCore Arcadia H (Leica Biosystems) following the manufacturer’s protocol in a medium size mold ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.