Labshake search
Citations for Leica :
251 - 299 of 299 citations for Goat Anti Dog IgA Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... The antibodies were washed in TBS three times for three minutes at a time before the Novolink post primary block (Leica biosystems) was applied for 30 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
-
bioRxiv - Immunology 2021Quote: Primary (1ry) Antibodies were incubated for one hour and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
-
bioRxiv - Pathology 2022Quote: ... slides were stained and processed using the 17B4 primary antibody on a Leica BOND-III autostainer (Leica Biosystems, Buffalo Grove, IL).
-
bioRxiv - Immunology 2019Quote: ... antibodies were used for staining with Bond Refine polymer staining kit on the Bond Max automated staining system (Leica Biosystems, Germany). All stained slides were dehydrated in ascending ethanol and xylene washes before coverslipping with cytoseal (Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL, Leica microsystems, GmbH, Wetzlar, Germany) at 20 × magnification ...
-
bioRxiv - Physiology 2020Quote: ... after which they were incubated with antibodies against GPT (AP7468b, Abgent; 1:200) and GPT2 (PAD854Mu01, Cloud-Clone Inc.; 1:200) antigens in BONDTM primary antibody diluent (AR9352, Leica Biosystems). For skeletal muscle fiber size visualization ...
-
bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
-
bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
-
bioRxiv - Genomics 2022Quote: Five μm sections of formalin-fixed paraffin-embedded tissues from postmenopausal women were stained with hematoxylin and eosin or with commercially available antibodies using the Leica Bond RX automated stainer (Leica Biosystems) and reviewed by gynecologic pathologists ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4μm sections of organoid blocks were stained with ARID1A antibody (ab182560, 1/3000) on the automated Leica BOND RX system (Leica Biosystems).
-
bioRxiv - Cell Biology 2023Quote: ... and immunostained using primary and secondary antibodies (listed in ESM Methods) and imaged using a TCS SP5 confocal microscope (Leica, Germany). Immunostaining of paraffin-embedded pancreas from mice with beta cell-selective deletion of Dnmt3a was performed according to [10 ...
-
bioRxiv - Immunology 2022Quote: ... Detection was performed using PowerVision Poly-HRP Anti-Rabbit IgG Novocastra (Leica Biosystems, Cat. PV6119) for 30-minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... transferred to TBST for 5 min and incubated with anti-rabbit poly-HRP-IgG (Leica Biosystems ...
-
bioRxiv - Cancer Biology 2019Quote: ... and completely covered with post-primary rabbit anti-mouse IgG (Leica Biosystems, Novolink, # RE7260-K) for 30 min at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated with PowerVision polymeric horseradish peroxidase (poly-HRP) anti-mouse IgG (Leica Biosystems) or PowerVision polyHRP anti-rabbit IgG for 2 hours at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and visualization systems (Omni Map anti-Rabbit, Ventana, Roche; Bond Polymer Refine Detection, Bond, Leica) conjugated with horseradish peroxidase ...
-
bioRxiv - Molecular Biology 2023Quote: ... and visualization systems (Bond Polymer Refine Detection, Bond, Leica; Omni Map anti-(rabbit,mouse,rat) Ventana ...
-
bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... was applied on tissue sections for 25 minutes incubation and the antigen-antibody binding was detected with Bond polymer refine detection (Leica Biosystems, DS9800). The tissue sections were covered with cover glasses ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were prepared at a thickness of 70 μm for imaging intrinsic fluorescence or 40 µm for antibody staining (Leica VT 1000S vibratome). For immunohistochemistry ...
-
bioRxiv - Neuroscience 2023Quote: ... Colocalization of the antibody immunofluorescence signal and endogenous fluorescence of NG2 glia was examined using the Leica Stellaris confocal platform (Leica Microsystems, Wetzlar, Germany). The immunopositive areas of the cortex surrounding the ischemic injury were further quantified using Fiji ImageJ software (NIH ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Glial fibrillary acid protein (GFAP) Iimmunohistochemistry was carried out using GFAP primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously (39–42).
-
bioRxiv - Neuroscience 2023Quote: Neuron specific enolase (NSE) immunohistochemistry was carried out using NSE primary monoclonal antibody and the Novocastra™ and Novolink DM polymer detection system (Leica Biosystems, UK) as described previously [54 ...
-
bioRxiv - Immunology 2023Quote: ... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
-
bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
-
bioRxiv - Pathology 2021Quote: ... using monoclonal mouse anti-DYS1 (Rod domain, no. NCL-DYS1; clone no. Dy4/6D3, Leica Biosystems, Wetzlar) as primary antibody ...
-
bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
-
bioRxiv - Cancer Biology 2020Quote: Five micron sections of tumors were immunolabeled with one of the following antibodies using a Leica Bond III automated immunohistochemistry instrument (Leica Biosystems, Buffalo Grove, IL): Rabbit anti-CD3 ...
-
bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... using Leica Bond III automated staining system with diaminobenzidine chromogen detection and the same antibodies as for clinical diagnostics (DS9800, Leica Biosystems, Buffalo Grove, IL). Hematoxylin and eosin stain was used for general histopathology ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were sealed with an anti-fade reagent (VECTASHIELD) for further imaging (Leica confocal microscope TCS SP8 DLS). For Thioflavin-S staining ...
-
bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
-
bioRxiv - Bioengineering 2024Quote: ... and anti-collagen I immunofluorescence images were taken by confocal laser scanning microscopy (DMI8 SP8, Leica, Wetzlar, Germany).
-
bioRxiv - Pathology 2023Quote: ... Diluted primary antibody (CST #8242 1:800; CST #99940, 1:150; CST #9718, 1:500; Abcam ab107099, 1:X; Leica P53-CM5P-L 1:200) was applied and slides were incubated at 4°C overnight ...
-
bioRxiv - Immunology 2020Quote: ... and rabbit anti-rat secondary (Vector) DAPI was used to stain nuclei in Bond Polymer Refine Detection (Leica Biosystems) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were mounted in anti-fade mounting medium supplemented with DAPI for nuclear staining and imaged by confocal microscopy (Leica SP5 X MP ...
-
bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
-
bioRxiv - Cell Biology 2020Quote: ... all washing procedures were done by pelleting at 500g x 5m and resuspended in Prolong anti-fade gold for mounting and imaging on an SP5 confocal microscope (Leica).
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
-
bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
-
bioRxiv - Physiology 2021Quote: ... blocked samples were incubated for 1 hr at room temp in buffer A containing mouse anti-dystrophin IgG1 (1:20, #NCL-DYS2, Novocastra / Leica, Buffalo Grove, IL). The sections were then washed 3 times for 15 min with PBS and incubated in buffer A containing Alexa 488 goat anti-mouse IgG1 (1:500 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The fixed small intestine was opened along the anti-mesenteric attachment and examined for lesions under a dissecting microscope (S6D, Leica Microsystems, Wetzlar, Germany) (x20) ...