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Citations for Leica :
251 - 294 of 294 citations for Galectin 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Fixed human brain tissue (prefrontal cortex, BA10) was sectioned at 50 m using a vibratome (Leica). The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Attached RONAs and human cortical assemblies were sectioned (25 μm) by cryostat (CM3050; Leica, Nussloch, Germany) and collected on SuperFrost Plus glass slides (Roth ...
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bioRxiv - Biophysics 2019Quote: ... were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems, Wetzlar, Germany), before 5 μl aliquots of the liposome/protein solution were applied to the grids ...
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bioRxiv - Neuroscience 2021Quote: ... Red light illumination was provided by 8 individually addressable LEDs (WS2812) attached to the objective (Leica Z6 APO coupled with a Leica PlanApo 2.0X 10447178) by a custom 3D-printed conical holder ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Plant Biology 2021Quote: ... Inflorescence stem subjected to GUS staining were then embedded in 8% agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Plant Biology 2021Quote: ... GFP and YFP signals were imaged on a Leica SP2 or Leica Stellaris 8 Laser Scanning Confocal Microscope (Leica Microsystems, Buffalo Grove, IL) at 48hpi ...
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bioRxiv - Neuroscience 2019Quote: ... Coronal slices of paraffin sections with 8 µm thickness were continuously cut using a Leica RM2255 microtome (Leica Biosystems Inc. Buffalo Grove, IL) and mounted on Superfrost Plus microscope slides (cat# 12-550-15 ...
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bioRxiv - Neuroscience 2020Quote: ... and the brain was cut into 400-um thick coronal sections using a vibratome (Leica VT1000S; Leica, speed 5, vibration frequency 7-8), while in bubbling ice cold ACSF (recipe as above ...
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bioRxiv - Plant Biology 2022Quote: ... Inflorescence stems subjected to GUS staining were then embedded in 8% (w/v) agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Neuroscience 2024Quote: ... High-resolution microphotographs (1024×1024 pixel resolution and 400Hz acquisition speed) were taken at 20X magnification using an inverted Stellaris 8 confocal microscope (Leica Microsystems, Wetzlar, Germany). Same laser gain ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Neuroscience 2022Quote: Paraffinized human optic nerves were cut in 5 μm sections using the microtome RM2155 (Leica, Wetzlar, Germany) and placed on 1 mm thick microscope slides (Marienfeld ...
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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bioRxiv - Neuroscience 2021Quote: ... 8–10 week old mice received a single impact onto the skull at bregma using an ImpactOne stereotaxic impactor (Leica Biosystems, Buffalo Grove, IL) with a 3-mm-diameter tip (velocity set at 4.0 m/s ...
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bioRxiv - Cell Biology 2023Quote: ... Cryosections (8 μm) were cut on a cryostat HM 505E (Microm, Walldorf, Germany) equipped with a CryoJane Tape-Transfer system (Leica Inc., Buffalo Grove, IL). For labeling ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Physiology 2020Quote: ... Sections of the arms and the central disk region (8 μm; transverse or horizontal) were cut using a microtome (RM 2145, Leica Microsystems [UK], Milton Keynes, UK) and mounted on chrome alum/gelatin coated microscope slides ...
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bioRxiv - Biophysics 2021Quote: ... Immersion Oil (Cargille laboratories, LDF, Code 387) was added onto the SU-8 layer before bringing the oil immersion objective (Leica, 63x, PL APO, 1.40 NA) manually in close proximity to the wafer surface ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were attached to SEM pin studs with conductive double-sided carbon tape and coated with 8 nm gold layers using a vacuum sputter coater (Leica EM ACE200 Low Vacuum Coater). The gold-coated samples were imaged using a Zeiss field emission scanning electron microscope (GeminiSEM 460 ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Neuroscience 2023Quote: ... Stained sections were analyzed with a fluorescence microscope (Keyence BZ-X810) or a laser scanning confocal microscope (Olympus Fluoview FV1000D, Leica TCS SP-8, or Carl Zeiss LSM880). Image analyses were performed with IMARIS (Oxford Instruments) ...
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bioRxiv - Neuroscience 2019Quote: ... 5μm formalin fixed paraffin embedded tissue sections of human frontal cortex were deparaffinized and processed using heat induced epitope retrieval with an EDTA-based buffer (Leica #AR9640) for 20 minutes and incubated with primary antibody for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded whole-tissue sections from decalcified human bone marrow core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously91 ...
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bioRxiv - Systems Biology 2023Quote: ... paraffin-embedded whole-tissue sections from decalcified human BM core biopsy specimens in a Bond RX automated tissue stainer (Leica Biosystems, Buffalo Grove, IL), as described previously 80,81,87,88 ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections from human and mouse spinal cord or DRGs were cut at 12 μm using a freezing microtome (CM1850; Leica, Buffalo Grove, IL, USA) and incubated at 4°C overnight with primary antibodies including ...