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Citations for Leica :
251 - 300 of 2083 citations for E 6 2 6 6 Trimethylcyclohex 2 en 1 yl hex 5 ene 2 4 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Cell Biology 2021Quote: ... was deposited on an EM copper grid (400-mesh, Plano G2400C, coated with 6 nm thick carbon produced with a Leica EM ACE600 sputter coater), incubated for 30-60 sec and manually blotted with filter paper ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...
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bioRxiv - Immunology 2023Quote: ... coverslips were mounted on aluminum stubs with carbon adhesive tabs and coated with 10 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on a FE-SEM (Zeiss Merlin ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
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bioRxiv - Bioengineering 2022Quote: ... the laser scanning setup was chosen (Leica Sp8 SMD, see suppl. video 2): a small square ROI of ca ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescent images were observed under confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Cell Biology 2021Quote: ... for cryosection preparations or in 2% agarose for sectioning with a vibratome (Leica). Sections were subsequently blocked using 1%BSA in TBS containing 0.05% Tween 20 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... glo-2 mutants were phenotyped on a DM5500 wide-field fluorescence microscope (Leica) for the absence of auto-fluorescent and birefringent gut granules ...
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bioRxiv - Neuroscience 2021Quote: ... Sections of 2 μm thickness were sliced on an ultramicrotome (Leica EM UC6) at −80°C and placed onto gridded coverslips (type 1.5 H ...
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bioRxiv - Microbiology 2021Quote: ... and sputter coated with ∼2 nm iridium (EM ACE600; Leica, Buffalo Grove, IL). Imaging was done using a Hitachi SU8230 field emission instrument at 0.8 kV using the low-angle backscatter and secondary electron detectors.
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bioRxiv - Neuroscience 2020Quote: ... Paraffin sections (2 µm in thickness) were generated using a sliding microtome (Leica). Prior to immunoreactions ...
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bioRxiv - Cell Biology 2020Quote: FRET imaging experiments were performed using an epifluorescence inverted microscope (DMIRE-2, Leica) with a PlanApo 40x oil immersion objective ...
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bioRxiv - Microbiology 2021Quote: ... Antigen retrieval was performed by epitope retrieval (ER) 2 solution (Leica, pH 9). The slides were then incubated with anti-E-cadherin ...
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bioRxiv - Neuroscience 2021Quote: ... Imaging was performed under anesthesia using 2-photon laser scanning microscope (SP8, Leica). SR-101 (1 μM applied on the cortex for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For HLA staining heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) was used ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems, Vienna) and processed at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by pre-treatment with BOND Epitope Retrieval Solution 2 (Leica Biosystems, AR9640) and protease (ACD ...
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bioRxiv - Cell Biology 2022Quote: ... Regions 2 and 3 were then further sectioned using a vibratome (VT1000S, Leica) set to produce 200 μm sections ...
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bioRxiv - Bioengineering 2022Quote: ... for all but CD206 which utilized AEC (Cat# K400511-2, Leica, Heidelberg, Germany). A hematoxylin (Cat #K800821-2 ...
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bioRxiv - Biochemistry 2023Quote: ... and coated by sputtering of 2-3 nm of Chromium (ACE600, Leica microsystems) prior to observation with a high-resolution Field-Emission SEM operating at 1.5 kV (GeminiSEM500 ...
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bioRxiv - Microbiology 2024Quote: ... Heat-induced antigen retrieval was performed using Epitope Retrieval 2 solution (Leica Biosystems) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to epitope retrieval with Bond ER solution 2 (Leica Biosystems, #AR9640). Antibody labeling was then accomplished by 4 sequential rounds of primary antibody incubation ...
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bioRxiv - Microbiology 2024Quote: ... After 20 minutes of HIER with EDTA (Bond Epitope Retrieval Solution 2, Leica), tissue was blocked with Ultracruz (sc-516214 ...
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cell Biology 2024Quote: ... and EB3 comets in single cells were imaged for 60 seconds (1 frame per 2 seconds) with a Leica DMi8 microscope (Leica). Only cells exhibiting both tdTomato and EYFP signals were included in the analysis ...
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bioRxiv - Immunology 2022Quote: ... Mouse tissues (at least 2 mm2/organ) were imaged by confocal microscopy (SP8, Leica) using 40x oil objective ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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bioRxiv - Systems Biology 2021Quote: ... Mice were anesthetized with 2% isoflurane in oxygen using a precision vaporizer (Leica Biosystems). In each group ...
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bioRxiv - Plant Biology 2021Quote: ... and then transferred at −90 °C into an AFS 2 freeze-substitution device (Leica). Grafts were incubated in a cryosubstitution mix containing only uranyl acetate 0.1 % in pure acetone for 30 h (freeze substitution and embedding protocol adapted from Nicolas et al. ...
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bioRxiv - Genetics 2021Quote: ... Stained 2-day-old animals were analysed by a vertical fluorescent microscope (DM2500, Leica). We selected the area between two pharyngeal bulbs to evaluate the number of β-amyloid deposits ...
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bioRxiv - Bioengineering 2020Quote: ... with 2 to 3 sections placed onto Leica X-tra adhesive microscope slides (Leica Biosystems Nussloch GmbH ...
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bioRxiv - Developmental Biology 2022Quote: Widefield images were acquired every 2 min with an M205 fluorescent dissection microscope (Leica) equipped with a PLANAPO 2.0x objective ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% FBS) using a Leica EM PACT2 high-pressure freezing unit (Leica Microsystems) with rapid transfer system at high pressure (2100 bar) ...
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bioRxiv - Developmental Biology 2022Quote: ... They were fixed by high pressure freezing with the EMPACT-2 system (Leica Microsystems). Cryosubstitution (FS ...
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bioRxiv - Cell Biology 2024Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Neuroscience 2021Quote: ... contusion was induced using a 2 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 1.25 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Plant Biology 2021Quote: ... 9.3% water in water-free acetone in a Leica AFS 2 (Leica Microsystems, Wetzlar, Germany) as described previously (Flechsler et al. ...