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Citations for Leica :
251 - 300 of 1648 citations for 7 Hydroxy 2 3 dihydro 1H cyclopenta c chromen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The frozen blocks were cryostat-sectioned into 7 μm sagittal sections (Leica). Sections were mounted on poly-L-lysine coated glass slides ...
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bioRxiv - Developmental Biology 2024Quote: ... and then serially cut into 7-µm sections on a microtome (Leica). Slides were immersed in 2N HCl for 15 min for DNA denaturation ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Physiology 2024Quote: ... Brains were cryosectionned (Leica CM3050, head at - 15°C chamber -20°C), first by coarsely trimming the anterior brain until the start of the SCN ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... One sample brain was sectioned using a cryostat (Leica 3050 S) and imaged with an epifluorescence microscope (Zeiss Axio Imager 2).
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bioRxiv - Cell Biology 2023Quote: ... One hundred µm sections were made using a vibratome (Leica #VT1200) as described here23 ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections of 7 μm in thickness were cut by freezing microtome (Leica, Germany). The sections were used for immunofluorescence according to a previously described method (Wang ...
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bioRxiv - Neuroscience 2021Quote: ... blocks were sectioned at 90 nm thickness on an ultramicrotome (Ultracut 7, Leica), mounted on copper grids ...
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bioRxiv - Immunology 2021Quote: ... 7-10 μm thick sections were prepared with a cryostat (Leica CM 1950). For the H&E staining ...
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bioRxiv - Cell Biology 2023Quote: ... Cryosections (typically 7-10 µm thickness) were created on a Cryostat (3050S, Leica) and the sliced tissues were collected on adhesive microscope slides (16005-110 ...
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bioRxiv - Plant Biology 2023Quote: ... Resin blocks were sectioned at 7 μm with a rotary microtome (Leica Biosystems), stained on glass slides with Toluidine Blue solution for 30 seconds ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen kidney sections were cut at 7 µm thickness in a cryostat (Leica) and mounted on MAS-coated glass slides (Matsunami) ...
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bioRxiv - Pathology 2023Quote: ... Cryosections 7 μm thick were made using a Leica CM1850 cryotome (Leica, Germany), placed on Superfrost Plus glass slides (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... 7 µm thick sections were prepared using a cryotome (Leica Biosystems, Wetzlar, Germany). To detect the transplanted mFB and to analyse Cx43 expression in the scar area ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Immunology 2021Quote: ... After fixation organs were stored in PBS till paraffin embedment and cut of serial sections on a microtome (2-3 µm; Leica RM 2035; Leica Instruments GmbH, Nuβloch, Germany). Sections were stained by hematoxylin and eosin (HE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The growth culture medium was changed every 2-3 days and cell morphology was regularly checked by visual inspection with phase-contrast microscopy (Leica DMIC, Leica Microsystem GMbH, Wetzlar, Germany). When the degree of confluence reaches approximately 80 % (every 4-6 days) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Neuroscience 2020Quote: ... The animals were placed in a stereotactic frame (Angle One; Leica, Germany) and bilateral injections were performed in the SC (ML ± 0.8 mm ...
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bioRxiv - Neuroscience 2023Quote: ... Under one fluorescence microscope(Leica dm6000B, Leica Microsystems Inc., Buffalo Grove, IL) or Zeiss LSM-710 inverted confocal microscope ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections at 7 µm thickness were cut on a cryostat (Leica Biosystems, Wetzlar, Germany), mounted on positively charged slides ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... Polymerised resin blocks were sectioned using an ultramicrotome (Ultracut 7, Leica Microsystems, Wetzlar, Germany) to generate 60 nm ultra-thin sections that were collected on 200 mesh copper grids ...
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bioRxiv - Neuroscience 2022Quote: ... Embedded samples were sectioned to 7 μm sections using a microtome (Leica RM2235, UK), and mounted on HistoBond slides (StatLab ...
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bioRxiv - Cancer Biology 2020Quote: ... Biomarker conditions were as follows: Ki67 (clone MM1 DO-7 mouse monoclonal antibody, Leica) was used at a 1:200 dilution with epitope retrieval solution 2 pre-treatment for 30 min ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... Whole plate acquisition was performed using a dissecting microscope and Cell Discoverer 7 (Leica). Images were captured with ZEN ...
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bioRxiv - Developmental Biology 2020Quote: 7 μm sections were cut on a Leica CM3050 S Research Cryostat (Leica Biosystems). Tissue sections were further fixed for 5 minutes in 3.2% PFA ...
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bioRxiv - Immunology 2023Quote: ... Ultrathin sections (approximately 80-100 nm) were prepared using an EM UC 7 (Leica) and contrasted with uranyl acetate followed by lead citrate ...
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bioRxiv - Developmental Biology 2024Quote: ... and then serially cut into 7 μm sections on a microtome (Leica, Wetzlar, Germany). Slides were stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Plant Biology 2024Quote: ... Sample sections of 5 to 7 μm thickness were cut using a microtome (Leica) and observed under a microscope (OLYMPUS ...
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bioRxiv - Bioengineering 2019Quote: ... A cryostat (Leica, −20°C) was used to cut the samples ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cell Biology 2020Quote: ... was used to section the blocks on a Leica Ultracut UCT (7) ultramicrotome (Leica Microsystems), which were collected onto copper grids ...
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bioRxiv - Plant Biology 2021Quote: ... Cross sections 7-10 μm thick were obtained using a microtome (Leica RM 2245, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... 7-10 μm sections from the nerves were cut using a cryostat (Leica Microsystems, Germany) and collected directly onto glass slides ...
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bioRxiv - Cancer Biology 2020Quote: ... Colony forming efficiency was determined after 7 days using Leica DMI8 inverted microscope (Leica Microsystems) and quantified using Image J software.
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bioRxiv - Cancer Biology 2022Quote: ... Cryosections of 7 µm were generated with a Leica cryostat (DB80 LX microtome blades, Leica) using Kawamoto tape62 (Section-lab ...
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bioRxiv - Neuroscience 2020Quote: ... Ultrathin sections (50 nm) were cut using a UC-7 ultramicrotome (Leica Microsystems, Vienna, Austria) and contrasted with UranyLess™ (Science Services ...