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Citations for Leica :
251 - 300 of 1989 citations for 7 Boc 3 chloro 5 6 7 8 tetrahydroimidazo 1 2 a pyrazine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... embryos were paraffin-embedded and serially sectioned at 3-5 µm (Leica microtome), before attachment to poly-L-lysine-coated glass slides (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
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bioRxiv - Cell Biology 2022Quote: A minimum of 5 fields per cover slip were imaged using a SP-8 Lightning Confocal Microscope (Leica) or CSU-W1 Spinning-Disk Confocal Microscope (Nikon) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Neuroscience 2020Quote: ... and 2 CaCl2) using a vibratome (Vibracut, Leica Biosystems, Germany) followed by incubation in carbogenized aCSF for 30 min at 36°C and then kept at room temperature (~21° C ...
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bioRxiv - Plant Biology 2021Quote: ... An upright confocal laser scanning microscope (TCS SP5 2; Leica) using a 40x apochromatic water immersion lens was used to visualized YFP fluorescence (excitation ...
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bioRxiv - Microbiology 2021Quote: ... and 2% water with an AFS (Leica microsystems, Vienna, Austria). After bringing the cells to RT they were washed in acetone and gradually infiltrated with epoxy resin (Agar Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mm sections were obtained using a vibratome (Leica, VT1200S) and immobilized using glue in a polystyrene-bottom dish (TPP ...
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bioRxiv - Biophysics 2023Quote: ... 2 mm sections were obtained using a vibratome (Leica, VT1200S) and immobilized using tissue seal (histoacryl blue ...
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bioRxiv - Microbiology 2019Quote: ... each for 30 min at room temperature and 100% for 2 × 1 h and 1 × 30 min) and dried in a Leica EM CPD030 (Leica Microsystems Belgium, Diegem, Belgium). The membranes were mounted on a stub and coated with 5 nm of carbon (Leica Microsystems Belgium ...
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bioRxiv - Bioengineering 2022Quote: ... and placed on a microscope slide to image with a confocal microscope (Leica Stellaris 5 confocal with DMI 8). The settings for fluorescent imaging were calibrated using a positive control ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were stained using a Leica BOND-MAX-TM autostainer with BondTM epitope retrieval 1 or 2 and the Bond Refine Detection Kit (Leica). Primary antibodies are listed in Supplementary Table S1.
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in dichloromethane and then transferred to a clearing solution (benzyl alcohol: benzyl benzoate (1:2)) and imaged with a confocal laser scanning microscope (SP8, Leica).
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Neuroscience 2019Quote: ... Pictures were taken with a Leica microscope (Axioscop 2, Leica GmbH).
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bioRxiv - Neuroscience 2021Quote: ... Slices were visualized on a resonant scanning 2-photon system (Leica) and Z-stack images were taken ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by pretreatment with Epitope Retrieval Solution 2 (AR9649, Leica Biosystems) for 20 minutes and then blocking buffer for 10-20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Panel 2 consisted of primary antibodies against CD3 (clone LN10, Leica), PD-1 (clone NAT105 ...
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bioRxiv - Immunology 2023Quote: ... Fura-2-AM was excited using a monochromatic light source (Leica) coupled into an inverted Leica DMI4000B microscope that was equipped with a 20x objective (HCX PL Fluotar 20x ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
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bioRxiv - Molecular Biology 2023Quote: ... sputter-coated with 2 nm carbon using ACE600 sputter coater (Leica).
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Cell Biology 2020Quote: ... acid alcohol (Leica) for 5 s ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Epitope Retrieval Solution 2 (ER2, Leica) antigen retrieval and stained with a prediluted anti-Ki-67 antibody (Mouse ...
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bioRxiv - Zoology 2020Quote: ... We obtained 2 μm sections in rotating microtome (RM 2265, Leica, Switzerland), which were stained with hematoxylin and eosin.
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bioRxiv - Cell Biology 2021Quote: ... Samples were stained in 2% aqueous uranyl acetate and lead citrate (Leica) and imaged on a Hitachi HT7800 transmission electron microscope ...
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bioRxiv - Pathology 2020Quote: ... Epitope retrieval was performed using Epitope Retrieval solution 2 (Leica Biosystems, Germany) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at −90°C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... and ddH2O (2 washes) with a Leica ST4020 Linear Stainer (Leica Biosystems). Tissues next underwent antigen retrieval by submerging slides in 3-in-1 Target Retrieval Solution (pH 9 ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II, Leica Microsystems, Wetzlar, Germany) for 10 min each ...
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bioRxiv - Developmental Biology 2023Quote: Worms (young adults) were fixed by HPF with EMPACT-2 (Leica Microsystems) and then freeze substituted in anhydrous acetone containing 1% OsO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Sections (2-µm thick) were cut using an UC7&2265 microtome (Leica). To visualize starch granules and protein bodies ...
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bioRxiv - Cell Biology 2023Quote: ... Tubes were loaded into an AFS-2 freeze-substitution machine (Leica Microsystems) and processed at -90°C for 72 h ...
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bioRxiv - Plant Biology 2024Quote: ... Sections (2-µm thick) were cut using a UC7&2265 microtome (Leica). To visualize starch granules and protein bodies ...
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bioRxiv - Neuroscience 2024Quote: ... All procedures were carried out using to Angle 2 software (Leica Biosystems) to set and locate coordinates ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).