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Citations for Leica :
251 - 300 of 1613 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Cancer Biology 2024Quote: ... immunostaining of formalin-fixed paraffin-embedded sections (2-4 μm) was performed with the Bond RX automated stainer (Leica Biosystems, Germany). Antigen retrieval was 20 mins at 100°C using the Bond Epitope Retrieval solution 2 (EDTA based buffer ...
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bioRxiv - Biophysics 2021Quote: ... 1 was reconstructed using the Leica Application Suite X Software (Leica Microsystems, Germany). Image enhancement of fluorescence micrographs for the figures was performed with the Adobe Photoshop CS4 (Adobe Systems ...
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bioRxiv - Cell Biology 2021Quote: ... on 1% phosphatidylcholine (sigma-aldrich) pre-coated 200μm deep flat carriers (Leica Biosystems), followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems ...
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bioRxiv - Plant Biology 2020Quote: ... Embedded tissues were sectioned at 1 µm using the Leica Ultracut UCT (Leica Microsystems Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... The blotted membranes were probed with anti-p53 antibody (1:500; Leica Biosystems) and anti-GAPDH antibodies (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections for F4/80 staining were retrieved using enzyme 1 solution (AR9551, Leica) for 10 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval (HIER) was performed using Epitope Retrieval Solution 1 (Leica), heated to 100°C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Heat-induced epitope retrieval (HIER) was performed using Epitope Retrieval Solution 1 (Leica), heated to 100°C for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with anti-vimentin antibody (SRL33, 1:400 dilution, Leica Biosystems). Biotinylated anti-mouse IgG secondary antibody and ABC avidin-biotin-DAB detection kit (Vector laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... for stepwise incubation with BOND Epitope Retrieval Solution 1 pH 6 (AR9961, Leica) at 100°C for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed samples were put into 1% OSO4 acetone solution for dehydration (Leica AFS2), and the temperatures control program was set as −90□ for 72 hrs. ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... The THP-1 macrophages were imaged using a SP5 confocal microscope (Leica Microsystems) with a magnification of 63X and a 1.4 numerical aperture (NA ...
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bioRxiv - Neuroscience 2022Quote: ... Semi-thin sections (1 μm) were cut on an ultramicrotome (EM UC7, Leica) and collected 2 mm distal to the eye ...
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bioRxiv - Molecular Biology 2024Quote: ... and imaged by confocal microscopy (CLSM-Leica-SP2, 1024x1024 resolution, 63x, airy=1). Channels were acquired sequentially ...
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bioRxiv - Immunology 2022Quote: ... sections were antigen retrieved with ER solution 1 (Leica Microsystems, catalog no. AR9961), protein block was applied for 30 mins (Leica Microsystems ...
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bioRxiv - Immunology 2023Quote: ... Setup #1 was further equipped with the beam splitters calcium cube FU2 (Leica), ZT405/488/561/647rpc and ZT405/488/532/647rpc (Chroma ...
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bioRxiv - Bioengineering 2023Quote: ... were afterwards stained for 30 min in 1% aqueous uranyl acetate (Leica, Germany) and 20 min in 3% lead citrate (Leica ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunofluorescence analysis with an anti-Ki67 mouse monoclonal antibody (Leica Biosystems, 1:100) or with an anti-pH3 (Ser 10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and antigen retrieval was performed using BOND Epitope Retrieval Solution 1 (Leica Biosystems) at 100°C for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed using Epitope Retrieval Solution 1 (Leica) and along with heating the tissue section to 100°C for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was done using Epitope retrieval buffer 1 (pH 6; Leica biosystems) for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... was diluted 1:250 in Bond Primary Antibody Diluent (Leica Biosystems, cat# AR9352), and BOND IHC Polymer Detection Kit (Leica Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Images were acquired with either 1) a Personal DeltaVision microscope system (Leica Microsystems) that includes an Olympus IX71 microscope ...
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bioRxiv - Bioengineering 2024Quote: ... After 1 hour incubation at 37 °C confocal microscope images (Leica, 10x objective) were taken of the entire gel surfaces in one representative focal plane.
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... 1 mM CaCl2 and 1.25 mM NaH2PO4 under a stereotaxic dissection microscope (Leica). Tissue samples were cut into small pieces and snap-frozen by placing the tissue on a strip of aluminum foil in pre-chilled 2-methylbutane on dry ice for ∼10 min ...
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bioRxiv - Genomics 2024Quote: ... anti-human CD8 clone 4B11 dilution 1:30 (MA1-80231, Leica Biosystems, Invitrogen), anti-human CD4 clone SP35 dilution 1:30 (MA5-16338 ...
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bioRxiv - Microbiology 2024Quote: ... After 15 min of HIER EDTA (Bond Epitope Retrieval Solution 1, Leica Biosystems), peroxide block was applied with a mouse Ig block and normal horse serum (cat# S-2000 ...
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bioRxiv - Immunology 2024Quote: ... 70% ethanol (1×) and ddH2O with a Leica ST4020 Linear Stainer (Leica Biosystems). The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9 ...
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bioRxiv - Cancer Biology 2024Quote: ... Wound closure was measured at regular intervals (0-hour, 3-hour, 6-hour, 9-hour) using an inverted microscope (Leica DMi1). The wound closure distances at different time points were normalized to the distance at 0hour to calculate the migration rate.
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bioRxiv - Neuroscience 2022Quote: ... in Epitope Retrieval Solution 2 (Leica, AR9640) for 20 minutes at 100°C ...
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bioRxiv - Cell Biology 2024Quote: ... Epitope Retrieval Solution 2 (Leica Cat#AR9640), Retrieval Solution Low pH (Agilent Cat#GV80511-2) ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2024Quote: ... gel blocks were cut into cuboid shapes (2×2×1.3 mm) with a vibratome (Leica VT1000S, Leica Biosystems GmbH). Scaffolds were then precisely inspected under a light microscope (Olympus BX53 ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used: NCAM (Leica Biosystems, NCL-L-CD56-504, 1:50) and MYCN (Novus Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle sections were also immunostained with human-specific spectrin (SPEC1-CE, 1:50, Leica) and human-specific lamin A/C (ab40567 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC, Novocastra, Leica) for primary hybridization step (overnight ...
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bioRxiv - Biophysics 2022Quote: ... the samples were coated with around 1 nm of either tungsten (ACE 200, LEICA). SEM imaging was performed using a GeminiSEM 500 microscope from Zeiss ...
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bioRxiv - Physiology 2022Quote: ... hydrated and heated in Novocastra Bond Epitope Retrieval Solution 1 pH 6.0 (ERI, Leica), and target antigens were detected using a Leica BOND-MAX robot.