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Citations for Leica :
2551 - 2600 of 2603 citations for 8 ETHOXYCARBONYL 6 METHOXY 7 METHYL 6H 1 2 5 OXADIAZOLO 4 3 E INDOL 3 IUM 3 OLATE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was performed using Leica DMI8 confocal microscope and HC PL APO CS2 20x objective (excitation, 488 nm, emission, 498 nm - 550 nm, pinhole size of 1 Airy Unit) (Leica Microsystems, Germany), pixel dimensions 0.48 μm px-1 ...
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bioRxiv - Plant Biology 2023Quote: 50 µm-thick cross sections were prepared from apical and basal internodes of Arabidopsis inflorescence stems at stage 1 (Altamura et al., 2001) by a vibrating blade microtome (Leica VT1200 S), stained with a 0.05% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation at 1:250 dilution for 30 minutes was followed by detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Slides were let dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired and the number of invaded cells counted using custom-made software further described in the next section ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Physiology 2024Quote: ... an area of each section (1 section/mouse) was acquired at 200x magnification using a confocal laser scanning microscope (Leica TCS SP8). Sequential scanning was employed using the 488 nm laser to visualize Alexa 488 and the 405 nm UV lasers to visualize DAPI staining ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Plant Biology 2020Quote: ... Thick sections (1 µm) of long shoots were cut with glass knives using an Ultracut R microtome (Leica Microsystems, Buffalo Grove, IL, USA) and stained with toluidine blue ...
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bioRxiv - Immunology 2021Quote: ... z-stacks were generated with a 20x objective at a z-step of 1 µm using a SP8 laser scanning confocal microscope (Leica Biosystems, Nußloch, Germany). Composite images were generated using ImageJ with Fiji distribution [31].
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Bioengineering 2019Quote: ... Confocal laser scanning microscopy of macroMs entrapment/dead cells at 4h and day 1 was performed with a Tandem confocal system (Leica TCS SP8 STED), equipped with a white light laser (WLL) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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bioRxiv - Neuroscience 2021Quote: images were obtained using the Axio Imager 1 microscope (Zeiss, Oberkochen, Germany) and confocal images were obtained with a laser scanning confocal microscope (Leica SPE, Mannheim, Germany) associated with a Leica LAS AF interface ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Microbiology 2023Quote: ... Z stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (33) ...
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bioRxiv - Physiology 2023Quote: ... ten H&E stained images of 1 mm2 (4,000,000 pixel2) were randomly extracted from the entire adipose tissue image using Aperio ImageScope (Leica Biosystems Imaging, Deer Park, IL) and analyzed with the MorphoLibJ collection of mathematical morphology methods and plugins using the image processing package FIJI [36] ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Z-stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (doi.org/10.15454/1.5572348210007727E12)54.54 ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Neuroscience 2024Quote: ... together with Opal 520/570/690 Fluorophore Reagent pack detection (1:1000, Cat #FP1487001KT/FP1488001KT/FP1497001KT) on the Leica BOND RX Fully Automated Research Stainer (Leica, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... immersed in 1-Hexadecene as cryoprotectant and immediately fixed by high pressure freezing (HPF) using the Leica EM ICE (Leica Microsystems GmbH, Germany). Freeze substitution and embedding of the HPF-fixed samples were conducted in a temperature-controlled device ...
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Bioengineering 2021Quote: ... or Toluidine Blue Stain, 1% w/v (Ricca Chemical, Arlington, TX) and scanned with a Leica Aperio ScanScope® CS2 (Leica, Buffalo Brove, IL) system ...
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bioRxiv - Neuroscience 2020Quote: ... the brain of each mouse extending from anterior to the DG to the cerebellum (from 0.22 to −5.34 μm from Bregma) was sectioned at 30 μm in a 1:9 series using a freezing microtome (Leica SM 2000 R Sliding Microtome). Sections were stored in 1xPBS with 0.01% sodium azide at 4°C until processing for IHC ...
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bioRxiv - Cancer Biology 2022Quote: Immunohistochemical staining was carried out on 1 μm tissue section in automated immunostainers (Discovery®XT, Roche, Penzberg, Germany, and Leica Bond, Wetzlar, Germany) using the streptavidin-peroxidase method ...
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bioRxiv - Plant Biology 2019Quote: ... the cotyledons were stained with 1 µg/ml propidium iodide and observed under a Confocal microscope (Carl Zeiss LSM710, Carl Zeiss LSM880 or Leica TCS SP8). For excitation ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated with CD8 mouse monoclonal antibody (clone 4B11, 1:150, 0.2 µg IgG2b/ml) from Novocastra (Leica Microsystems, Newcastle Upon Tyne, UK) for 45 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... the thecal plates of the dinoflagellates were stained with the fluorescent dye Calcofluor white M2R at a final concentration of 10–20 mg mL−1 (Fritz & Triemer, 1985) and examined under epifluorescence (lamp 50W) using a Leica DM IRB (Leica Microsystemps GMbH, Wetzlar, Germany) inverted microscope ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections at 1 µm were prepared using the resin-embedded buds using a glass knife on an EM UC6 ultramicrotome (Leica Microsystems GmbH, Wetzlar, Germany). Sections were mounted on glass slides ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Sections were then stained for Ly6G (clone 1A8) at a previously optimized dilution (1/60000) for 30 min at room temperature with the autostainer Bond Rx (Leica Microsystems GmbH, Wetzlar, Germany). The IHC sections were visualised with 3,3’Diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... Larvae were then positioned and oriented laterally to acquire two z-stacks of 30 optical sections (ΔZi, Zi-1 = 683.46μm) of the gastrointestinal tract using a 4X objective (Leica HC PL FLUOTAR 4X/0.13 DRY) at 10 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
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bioRxiv - Immunology 2023Quote: ... Frozen brains were then embedded in Epredia™ M-1 Embedding Matrix (Thermo Ficher Scientific) and cut in coronal sections using a cryostat (Leica CM3050S, Mannheim, Germany), and mounted on slides for either fluorescence immunocytochemistry (IF ...
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bioRxiv - Neuroscience 2024Quote: Immuno-stained anti-rabbit Iba-1 brain sections were imaged at a 40x oil objective using a confocal imaging system (Leica Microsystems, Deerfield, IL, USA) to further investigate microglia structure and morphology ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... was performed by the VCU Cancer Mouse Models Core Laboratory with the Leica Bond RX auto-stainer using heat-induced epitope retrieval buffer 1 (Leica, Sodium Citrate buffer, pH 6.0). Stained slides were then imaged on the Vectra Polaris (Akoya Biosciences) ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Cancer Biology 2021Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope by a senior pathologist (T.J.H.) ...
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bioRxiv - Cancer Biology 2023Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope (TH) ...