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Citations for Leica :
201 - 250 of 675 citations for Son of sevenless homolog 1 SOS1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and hematoxylin counterstain.
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bioRxiv - Genomics 2024Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with secondary antibodies and visualization were performed according to the manufacturer’s protocol (Novocastra Peroxidase Detection System, Leica). After each step ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody binding is detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica). Slides were scanned with an AT2 (Leica ...
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bioRxiv - Physiology 2024Quote: ... Whole slide images of antibody-labeled tissues were captured on an Aperio VERSA 200 (Leica Biosystems, Vista, CA) at the Vanderbilt Digital Histology Shared Resource (DHSR) ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the samples were embedded in a mixture of OCT and water at a ratio of 1:1 and sectioned at 10 μm thickness with freezing microtome (Leica). The mRNA detection by in situ hybridization was performed as previously described (Lauter et al. ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was trimmed to less than 1 × 1 mm and one side was exposed using an ultramicrotome (UC7, Leica), then turned downwards to be remounted to a metal pin with conductive silver epoxy (CircuitWorks ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Neuroscience 2021Quote: ... microglial density quantifications and Iba-1:P2RY12 or Iba-1:TMEM119 colocalization analysis 40 µm free-floating sections were cut with a sliding microtome (Leica) and stained with relevant antibodies in 10% normal goat serum (NGS ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The gonadal tissues for histology were dehydrated in a tissue processor (TP 1020-1-1, Leica Microsystems Nussloch GmbH, Germany) and thereafter embedded in paraffin ...
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bioRxiv - Neuroscience 2022Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (25 μm-thick sections) using a Leica Cryostat (Leica, CM1860). For immunofluorescence staining ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
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bioRxiv - Immunology 2021Quote: ... anti-CD20 (clone L26, 1:200, Leica Biosystem), anti-CD31 (clone PECAM-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... CK14 (Novocastra/Leica; 1/50; NCL-L-LL02), CK8/18 (Novocastra/Leica ...
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bioRxiv - Developmental Biology 2020Quote: ... γ-sarcoglycane (1/150, G-SARC-CE, Leica biosystems), or with 4–15% Criterion™ TGX™ Precast Midi Protein Gel (5671084 ...
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bioRxiv - Immunology 2021Quote: ... or BOND Epitope Retrieval 1 (AR9961, Leica Biosystems) during 20 minutes for the adaptive and innate panel ...
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bioRxiv - Cell Biology 2021Quote: ... β-dystroglycan (1:50, LCL-b-DG, Leica Biosystem) and Ki67 (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... GFAP (anti-GFAP (mouse, GA5, Leica, 1:200) and IBA1 (1:1000 Wako ...
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bioRxiv - Cancer Biology 2022Quote: ... p53 (1:2500, Leica Biosystems, P53-PROTEIN-CM5), p21 (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... p53 (1:500, Leica Biosystems, NCL-p53-505), p19 (1:250 ...
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bioRxiv - Immunology 2022Quote: ... and CD163 (Leica Microsystems, 10D6, 1:20 dilution). Subsequent visualization was performed using Akoya Opal fluorophores (620 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-Emerin (Leica NCL-Emerin; 1:1000). Secondary antibodies used ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-Vimentin (mouse, Leica, 1:100, cat#PA0640); anti-FAP (mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... P53 stabilization upon doxorubicin and Nutlin-3a treatment was confirmed by Western blotting using p53 CM5 antibody (Leica Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Cancer Biology 2024Quote: ... four-micron formalin fixed paraffin embedded (FFPE) tissue sections were incubated with the primary anti-ER 6F11 antibody (Leica) for 20 minutes ...
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bioRxiv - Pathology 2024Quote: ... Visualization of the primary antibody binding to tissue section was performed using BOND Polymer Refine Detection (DS9800, Leica Biosystems). For CGRP immunolocalization ...
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bioRxiv - Biochemistry 2022Quote: ... 36 h post-transfection both the cell lines were trysinised and co-cultured in 1:1 ratio for 12 h followed by fixing permialization and subsequent imaging using fluorescence microscope (Leica Microsystems) and subsequent analysis by ImageJ software.
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Neuroscience 2021Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (18-20 μm-thick sections) using a Leica Cryostat (Leica, CM1850). Plated hSS cultures on glass coverslips were fixed in 4% PFA at room temperature for 10 minutes and washed with then rinsed twice for 5 minutes with PBS.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Bioengineering 2020Quote: ... 0.52 mM H2O2) (1:1 v/v) SHG images were acquired with a MaiTai multi-photon laser equipped confocal microscope (Leica SP8). SHG signal was collected with HyD detector between 437-453 nm (λex 880 nm ...
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bioRxiv - Genomics 2022Quote: ... cells were resuspended in 1× PBS and placed on an agarose pad (1% agarose in 1× PBS) and imaged with a laser scanning Leica TCS SP5 II confocal microscope (Leica Microsystems).
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bioRxiv - Plant Biology 2024Quote: ... The samples were transferred to a 1:1 (v/v) solution of 100% ethanol and solution A (Leica Historesin Embedding kit) and kept for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were washed and the brains were mounted in Slowfade and imaged at a confocal microscope (Leica SP8) using a 63x glycerol objective ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated for 60 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: Assessment of Aβ deposition was carried out by immunohistochemical staining of paraffin-embedded sections (3–5 μm-thick) with a mouse monoclonal (S6 F/3D) anti-Aβ antibody (Leica Biosystems Newcastle Ltd ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were incubated for 30 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... IHC staining for Ki67 and MGP antibodies was performed using Bond III IHC autostainer for full Automated Immunohistochemistry (Leica biosystems). Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica ...
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bioRxiv - Immunology 2024Quote: ... The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes: CD20 (Leica Biosystems PA0200), 1:1 with CF488 ...
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bioRxiv - Genomics 2024Quote: ... Clinical determination of hormone receptor status and HER2-amplification status in the 79-patient validation cohort was performed at the Clinical Laboratory Improvement Amendments (CLIA)-certified MGH Clinical Immunohistochemistry and MGH Center for Integrated Diagnostics laboratories following standard protocols using monoclonal antibody clone 6F11 for ER and clone 16 for PR (Leica Microsystems ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then washed three times with 1X PBS and incubated with geminin-L-CE primary antibody (Leica Biosystems) for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... The grids with non-activated or IL33-activated (in total of 1 hr activation) human eosinophils were vitrified in liquid ethane using a Leica EM GP 1 (Leica Microsystems, Germany). The Leica EM GP 1 plunger was set to 37 ℃ ...