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Citations for Leica :
201 - 250 of 389 citations for Recombinant Human Interleukin 3 Receptor Alpha Low Affinity His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Positive nuclei (Ki67) and stained area (cleaved caspase 3) were analyzed using the ImageScope software (Leica Biosystems). Quantification is average of 3 sections ...
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bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
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bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
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bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...
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bioRxiv - Cell Biology 2022Quote: ... and images were acquired using a 3-channel TCS SP2 laser-scanning confocal microscope (Leica Mycrosystems, Germany) and STELLARIS 8 ...
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bioRxiv - Molecular Biology 2023Quote: 2.8 × 105 A549 or A549-IFITM3 cells were seeded on carbon- coated 3 mm sapphire discs (Leica) in a 6-well plate in complete growth medium (DMEM-F12 ...
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bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
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bioRxiv - Neuroscience 2023Quote: ... the puncture sample was placed in an aluminum specimen carrier (Ø = 3 mm; Leica Microsystems, Wetzlar, Germany) and underwent freezing in a high-pressure freezer (HPM100 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
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bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...
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bioRxiv - Microbiology 2021Quote: ... Confocal imaging was performed with a Leica TCS SP8 STED 3× laser scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 100×/1.4 Oil HC PL APO CS2 STED White objective ...
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bioRxiv - Neuroscience 2022Quote: ... then following fixation they were imaged with an inverted confocal microscope with the laser power increasing for 0.2 to 3% with 0.2% intervals (Extended Data Fig. 1a, b, SP8 TCS with HyD detectors, Leica). Laser power was linearly controlled by ATOF ...
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bioRxiv - Plant Biology 2022Quote: ... leaves were harvested at 3 dpi for aniline blue/calcofluor staining and analysis by epi-fluorescence microscopy (Leica DM6B ...
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bioRxiv - Plant Biology 2022Quote: ... GUS-stained leaves were embedded in 3% agarose and sectioned with a vibrating blade microtome (Leica VT1000 S). Images were taken using ZEISS Axio Observer.
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bioRxiv - Neuroscience 2023Quote: ... the ISH-mounted specimen was first loaded into a pre-tilted specimen block (Leica; Figure 3 J&K). The block was then placed into a Leica VCT-100 cryo-transfer system under liquid nitrogen to facilitate transfer into the pre-cooled cryo-FIB/SEM cryo-substage.
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bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
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bioRxiv - Cell Biology 2023Quote: ... The cryosection ribbon (∼3 mm long) was then attached to the grid by operating the Crion (Leica Microsystems) in “charge” mode for ∼30 seconds (Pierson et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in blocks of 3% agarose and sectioned in 100 µm slices on a vibrating blade microtome (Leica) or mounted as whole droplets.
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bioRxiv - Physiology 2023Quote: ... and incubated for 3 x 1 h in Xylene in an automatized system (TP1020 Tissue Processor, Leica Biosystems). Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome ...
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bioRxiv - Neuroscience 2019Quote: ... 5μm formalin fixed paraffin embedded tissue sections of human frontal cortex were deparaffinized and processed using heat induced epitope retrieval with an EDTA-based buffer (Leica #AR9640) for 20 minutes and incubated with primary antibody for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
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bioRxiv - Cancer Biology 2021Quote: ... Each wash was performed for 3 min at room temperature with repetitive dipping using a linear stainer (Leica, #ST4020). Slides were transferred for heat-induced epitope retrieval to a PT module (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: Fertilized ovaries from B73 plants were collected 3 days after pollination (DAP) and sliced using a Vibratom (Leica VT1000E) to create 200 to 225 μm sections ...
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bioRxiv - Cancer Biology 2020Quote: ... two sections of formalin-fixed paraffin-embedded tissues of FMC with 3 µm thickness (Microtome Leica RM2135, Newcastle, UK) were prepared ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Neuroscience 2021Quote: ... Three (3) brains per group were later sectioned with a cryostat (Cryocut 1800 Cryostat, Leica Biosystems, Vista, CA, USA) at 25 μm thickness in the coronal plane ...
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bioRxiv - Immunology 2020Quote: ... Lung tissues were embedded in 3% LMA and 100μm-thick sections were cut at the vibratome (Leica VT 1200S). Sections were placed on microscope slides ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea conidia and germlings exposed to 3 μM NCR044.1 was also imaged using fluorescence confocal microscopy (Leica SP8-X)39 with an excitation and emission wavelength of 480 and 510-566 nm ...
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bioRxiv - Genomics 2021Quote: ... Formalin-fixed agarose/paraffin-embedded sections (3-5µm) were manually cut from blocks with a microtome (Leica RM 2145) and transferred to glass slides (Superfrost ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...