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Citations for Leica :
201 - 250 of 259 citations for Rabbit HRP FOXA2 Monoclonal Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... The primary antibodies were detected using the Bond Polymer Refine (DAB) Detection Kit (Leica Biosystems; Buffalo Grove, IL). Positive control mouse tissues for all antibodies were run in addition to negative controls performed using rat or rabbit isotype IgG.
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and hematoxylin counterstain.
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bioRxiv - Genomics 2024Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with secondary antibodies and visualization were performed according to the manufacturer’s protocol (Novocastra Peroxidase Detection System, Leica). After each step ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody binding is detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica). Slides were scanned with an AT2 (Leica ...
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bioRxiv - Physiology 2024Quote: ... Whole slide images of antibody-labeled tissues were captured on an Aperio VERSA 200 (Leica Biosystems, Vista, CA) at the Vanderbilt Digital Histology Shared Resource (DHSR) ...
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bioRxiv - Neuroscience 2024Quote: ... or secondary antibodies (Table 1) were acquired using the Fast Lifetime Contrast (FALCON) enabled tau-STED module (Leica) with the time-gate on HyD-X detectors adjusted between 0.1 and 6 nanoseconds.
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bioRxiv - Developmental Biology 2020Quote: ... P53 stabilization upon doxorubicin and Nutlin-3a treatment was confirmed by Western blotting using p53 CM5 antibody (Leica Biosystems).
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bioRxiv - Immunology 2021Quote: ... Triple immunostainings were performed by incubating the same sections with CD8 antibody (clone 4B11, 1:50 pH9, Leica Novocastra) and anti-mouse Alexa Fluor 488-coniugated secondary antibody (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Bioengineering 2024Quote: ... Dystrophin detection (both full-length and µDystrophin) has been performed by the antibody DysB (NCL-DYSB, Leica, 1:20) and its expression has been quantified by total protein normalization.
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bioRxiv - Cancer Biology 2024Quote: ... four-micron formalin fixed paraffin embedded (FFPE) tissue sections were incubated with the primary anti-ER 6F11 antibody (Leica) for 20 minutes ...
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bioRxiv - Pathology 2024Quote: ... Visualization of the primary antibody binding to tissue section was performed using BOND Polymer Refine Detection (DS9800, Leica Biosystems). For CGRP immunolocalization ...
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bioRxiv - Microbiology 2024Quote: ... CD34 antibody was applied at ambient temperature for 30 min (1:50, cat# NCL-L-END, Leica, Bannockburn, IL) followed by an anti-mouse secondary antibody conjugated to HRP and visualized with Opal 690 (cat#OP-001006 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were washed and the brains were mounted in Slowfade and imaged at a confocal microscope (Leica SP8) using a 63x glycerol objective ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated for 60 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) (. Cells were imaged using a Leica DMi8 confocal microscope (63x ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies were used at the indicated dilution and indicated antigen retrieval method: Ki67 (CST 12202, 1:1000, ER2 Leica), CD8a (eBioscience 14-0808-82 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were incubated for 30 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... IHC staining for Ki67 and MGP antibodies was performed using Bond III IHC autostainer for full Automated Immunohistochemistry (Leica biosystems). Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica ...
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bioRxiv - Immunology 2024Quote: ... The following primary antibodies were used at the consequence and with indicated dilutions and CF dyes: CD20 (Leica Biosystems PA0200), 1:1 with CF488 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then washed three times with 1X PBS and incubated with geminin-L-CE primary antibody (Leica Biosystems) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibody was used: anti CD31 (CST, 3528, clone 89C2, 1:1600, antigen retrieval Leica ER1 AR9961 for 30min).
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bioRxiv - Cell Biology 2020Quote: ... The antibodies were washed in TBS three times for three minutes at a time before the Novolink post primary block (Leica biosystems) was applied for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Immunology 2021Quote: Primary (1ry) Antibodies were incubated for one hour and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
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bioRxiv - Pathology 2022Quote: ... slides were stained and processed using the 17B4 primary antibody on a Leica BOND-III autostainer (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL, Leica microsystems, GmbH, Wetzlar, Germany) at 20 × magnification ...
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bioRxiv - Physiology 2020Quote: ... after which they were incubated with antibodies against GPT (AP7468b, Abgent; 1:200) and GPT2 (PAD854Mu01, Cloud-Clone Inc.; 1:200) antigens in BONDTM primary antibody diluent (AR9352, Leica Biosystems). For skeletal muscle fiber size visualization ...
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bioRxiv - Immunology 2021Quote: ... Nucleocapsid Antibody (SINO BIO, 40143-R019) through LEICA BOND RX 1h RT and developed with Bond Polymer Refine Detection (Leica, DS9800). Brightfield images were acquired through an Aperio Scanscope System CS2 microscope and an ImageScope program (Leica Biosystem ...
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bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... and immunostained using primary and secondary antibodies (listed in ESM Methods) and imaged using a TCS SP5 confocal microscope (Leica, Germany). Immunostaining of paraffin-embedded pancreas from mice with beta cell-selective deletion of Dnmt3a was performed according to [10 ...
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bioRxiv - Genomics 2022Quote: Five μm sections of formalin-fixed paraffin-embedded tissues from postmenopausal women were stained with hematoxylin and eosin or with commercially available antibodies using the Leica Bond RX automated stainer (Leica Biosystems) and reviewed by gynecologic pathologists ...
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... was applied on tissue sections for 25 minutes incubation and the antigen-antibody binding was detected with Bond polymer refine detection (Leica Biosystems, DS9800). The tissue sections were covered with cover glasses ...
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bioRxiv - Cancer Biology 2024Quote: The assay consisted of the following antibodies and corresponding dilution factor: CK AE1/AE3 (Leica Biosystems, ref. AE1/AE3-601-L-CE) was applied at 1:250 for 28 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared at a thickness of 70 μm for imaging intrinsic fluorescence or 40 µm for antibody staining (Leica VT 1000S vibratome). For immunohistochemistry ...
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
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bioRxiv - Neuroscience 2023Quote: ... Colocalization of the antibody immunofluorescence signal and endogenous fluorescence of NG2 glia was examined using the Leica Stellaris confocal platform (Leica Microsystems, Wetzlar, Germany). The immunopositive areas of the cortex surrounding the ischemic injury were further quantified using Fiji ImageJ software (NIH ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...